Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,515,125	A→G	T122A (ACC→GCC)	ydcV →	putative ABC transporter membrane subunit YdcV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,515,125	0	A	G	100.0%	199.9 / NA	57	T122A (ACC→GCC)	ydcV	putative ABC transporter membrane subunit YdcV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/36);  total (21/36)

CATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGT  >  NC_000913/1514988‑1515260                                                                                                                                          |                                                                                                                                        cATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGcc                                                                                                                                       <  2:1726251/140‑1 (MQ=255) cATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGcc                                                                                                                                       <  1:1986612/140‑1 (MQ=255)         gctcTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTaa                                                                                                                               <  2:1520346/137‑1 (MQ=255)               gtgtTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCAt                                                                                                                         <  2:2094337/140‑1 (MQ=255)               gtgtTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCAt                                                                                                                         >  1:1899071/1‑140 (MQ=255)                         cGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCAt                                                                                                                         <  1:1961925/130‑1 (MQ=255)                         cGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCAt                                                                                                                         >  2:1961925/1‑130 (MQ=255)                         cGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAg                                                                                                               >  1:1825972/1‑140 (MQ=255)                          gCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGTTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGc                                                                                                              >  1:500289/1‑140 (MQ=255)                          gCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGc                                                                                                              >  2:1413953/1‑140 (MQ=255)                          gCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTATGGCGCTATTAGCCGCCTTTAAGACCATCAATCTGGAGc                                                                                                              >  2:1168617/1‑140 (MQ=255)                          gCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTATCACTGGTATGGTGTTATTAGCCGCATTTAAAACCATCAATCTGGAGc                                                                                                              >  2:672726/1‑140 (MQ=255)                               cAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTTAAACCATCAATCTGGAGCCGGGa                                                                                                         <  1:241061/140‑1 (MQ=255)                                    gCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGAttttt                                                                                                    <  2:1746082/140‑1 (MQ=255)                                     ccGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTc                                                                                                   <  1:1925670/140‑1 (MQ=255)                                      cgcgCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCa                                                                                                  <  2:546347/140‑1 (MQ=255)                                         gcTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCa                                                                                               <  2:180577/140‑1 (MQ=255)                                           tgtgGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATc                                                                                             >  2:236582/1‑140 (MQ=255)                                                         tttttCGGCAAAAACTCCATTTAGCCGTTACTGCTGCCGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGc                                                                               <  1:659401/140‑1 (MQ=255)                                                          ttttCGGCAAAAACGGCATTTCTCTTTTAGTGCTGCTTCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCg                                                                              <  1:1066928/140‑1 (MQ=255)                                                          ttttCGGCAAAAACGCCTTTTCGCTGTTACTACTGCTGCCCATTGCGCGGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCg                                                                              <  2:891097/140‑1 (MQ=255)                                                          ttttCGGCAAAAACGCAATTTCTCTGTTACCCCTGCTGCACATTGCGCAGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCg                                                                              <  2:1189288/140‑1 (MQ=255)                                                            ttCGGCAAAAACGCCCTTTCGCCGTGACTTCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGAc                                                                            <  1:944973/140‑1 (MQ=255)                                                            ttCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGAc                                                                            <  1:1413953/140‑1 (MQ=255)                                                            ttCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGAc                                                                            <  2:1899071/140‑1 (MQ=255)                                                               ggCAAAAACGCCATTTTGCTGTAACTGCTGAGGCCGATTCCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACttt                                                                         <  2:1137198/140‑1 (MQ=255)                                                               ggCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACttt                                                                         <  2:2029881/140‑1 (MQ=255)                                                                   aaaaCGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGAATTTTTCACCATCGCGGTCGGTCATGCGACTTTTtgt                                                                     >  1:889495/1‑140 (MQ=255)                                                                     aaCGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTgtgt                                                                   <  1:1976421/140‑1 (MQ=255)                                                                          cATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTtg                                                              <  1:236582/140‑1 (MQ=255)                                                                          cATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTtg                                                              <  1:2341264/140‑1 (MQ=255)                                                                          cATTTCGCTGTTACTCCTGCTGTCCATTGCGCTGCCGCGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTtg                                                              <  1:672726/140‑1 (MQ=255)                                                                            tttCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTtgtg                                                            <  1:1968342/140‑1 (MQ=255)                                                                                    ttACTGCTGCTGCCCATTGCGCAGCTGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTaa                                                    <  1:746978/140‑1 (MQ=255)                                                                                      aCTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTgtggtg                                                         <  1:1783402/133‑1 (MQ=255)                                                                                      aCTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTgtggtg                                                         >  2:1783402/1‑133 (MQ=255)                                                                                            gctgcCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCa                                            <  1:2143646/140‑1 (MQ=255)                                                                                              tgcCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATc                                          >  2:2439703/1‑140 (MQ=255)                                                                                                             cGGGCATTGTCACTGGTCTGGCGTTGTTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTcgccgc                           <  1:1200054/140‑1 (MQ=255)                                                                                                               ggCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTcgccgc                           >  2:230706/1‑138 (MQ=255)                                                                                                               ggCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTcgccgc                           <  1:230706/138‑1 (MQ=255)                                                                                                               ggCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCAc                         <  2:500289/140‑1 (MQ=255)                                                                                                               ggCATTGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCAc                         <  2:1825972/140‑1 (MQ=255)                                                                                                               ggCATTGTCACTGGTCTGGCGTTATGAGCTGCCTTTAGAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCAc                         <  1:821942/140‑1 (MQ=255)                                                                                                                   ttGTCACTGGTCTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCAcctcc                     <  2:2066526/140‑1 (MQ=255)                                                                                                                   ttGTCACTGGTCTGGAGTTATTAGCAGCCATTAAAACAATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCAcctcc                     <  1:1168617/140‑1 (MQ=255)                                                                                                                              cTGGCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGt          >  1:1752511/1‑140 (MQ=255)                                                                                                                                ggCGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTg        >  2:1506599/1‑140 (MQ=255)                                                                                                                                ggCGTTATTAGCAGCCTTTAAAACCATCACTCTGCAGCCGGGGTTTTTCACCATCGTGGTCGGACATGCGTCTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTg        >  2:2372153/1‑140 (MQ=255)                                                                                                                                  cGTTATTAGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAg      <  2:374501/140‑1 (MQ=255)                                                                                                                                    ttattaGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGc    <  1:263657/140‑1 (MQ=255)                                                                                                                                    ttattaGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGc    <  1:1506599/140‑1 (MQ=255)                                                                                                                                      attaGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTGGTTGTGGTGTTTAACAATGTCATCGCACGTTTTCCCCGCACCTCCTGGAGTCTGGTTGAGGCGt  >  1:1079988/1‑140 (MQ=255)                                                                                                                                      attaGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAATAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGt  >  1:1681758/1‑140 (MQ=255)                                                                                                                                      attaGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACCATGTCATCGCCCATTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGt  >  2:1043789/1‑140 (MQ=255)                                                                                                                                      attaGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGt  >  1:395477/1‑140 (MQ=255)                                                                                                                                      attaGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGt  >  1:195440/1‑140 (MQ=255)                                                                                                                                      attaGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGt  >  1:155760/1‑140 (MQ=255)                                                                                                                                      attaGCCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGt  >  2:599516/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGT  >  NC_000913/1514988‑1515260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑