Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,332,680	(C)6→7	coding (56/966 nt)	yhbE ←	inner membrane protein YhbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,332,674	1	.	C	100.0%	217.3 / NA	62	A21G (GCG→GGG)	yhbE	inner membrane protein YhbE
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (25/37);  total (25/37)

CGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGT  >  NC_000913/3332536‑3332806                                                                                                                                            |                                                                                                                                      cGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑‑c                                                                                                                                     <  1:684164/140‑1 (MQ=255)   aaaCACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑‑ccc                                                                                                                                   <  1:606042/140‑1 (MQ=255)      cacGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑‑cccccc                                                                                                                                <  2:88755/140‑1 (MQ=255)      cacGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑‑cccccc                                                                                                                                <  1:1325994/140‑1 (MQ=255)      cacGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑‑cccccc                                                                                                                                <  2:1500697/140‑1 (MQ=255)      cacGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑‑cccccc                                                                                                                                <  2:1590605/140‑1 (MQ=255)       acGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑‑ccccccc                                                                                                                               >  1:129412/1‑139 (MQ=255)        cgcgTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑‑ccccccca                                                                                                                              <  1:1210634/140‑3 (MQ=255)           gTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑‑cccccccagca                                                                                                                           <  1:1581437/140‑6 (MQ=255)           gTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑‑cccccccagca                                                                                                                           <  2:254730/140‑6 (MQ=255)              aTGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAAt                                                                                                                         >  1:271034/1‑140 (MQ=255)                ggcggcAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTg                                                                                                                       <  2:437377/140‑1 (MQ=255)                    gcAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCtgt                                                                                                                   >  2:473932/1‑140 (MQ=255)                        ccTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTgtggtg                                                                                                               <  1:871347/140‑1 (MQ=255)                        ccTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTgtggtg                                                                                                               <  2:1090195/140‑1 (MQ=255)                        ccTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTgtggtg                                                                                                               <  1:560049/140‑1 (MQ=255)                        ccTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTgtggtg                                                                                                               <  2:761736/140‑1 (MQ=255)                         ctctTCTTCACCGCAGGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGa                                                                                                              <  1:240164/140‑1 (MQ=255)                           cttcttCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAgc                                                                                                            >  2:1549088/1‑140 (MQ=255)                              cttcACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGcc                                                                                                         <  1:473932/140‑1 (MQ=255)                                   ccGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAg                                                                                                    >  1:496861/1‑140 (MQ=255)                                    cGCAAGAATGGCACCCAGGTCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGa                                                                                                   <  2:994864/140‑1 (MQ=255)                                    cGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGa                                                                                                   <  2:406119/140‑1 (MQ=255)                                      cAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGaat                                                                                                 >  1:908856/1‑140 (MQ=255)                                                 cccAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAAtgcctg                                                                                      >  2:712031/1‑140 (MQ=255)                                                    aGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATgcctgcct                                                                                   >  2:696470/1‑140 (MQ=255)                                                     ggCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGcctgcctg                                                                                  <  1:1136923/140‑1 (MQ=255)                                                     ggCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGcctgcctg                                                                                  <  1:712031/140‑1 (MQ=255)                                                     ggCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGcctgcctg                                                                                  >  1:1544727/1‑140 (MQ=255)                                                     ggCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTAGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGcctgcctg                                                                                  <  2:1153217/140‑1 (MQ=255)                                                      gCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGATGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCctgcctgc                                                                                 <  2:1544727/140‑1 (MQ=255)                                                      gCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGcctgcctg                                                                                  >  1:1549416/1‑139 (MQ=255)                                                      gCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGcctgcctg                                                                                  <  2:1549416/139‑1 (MQ=255)                                                        cAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCctgctg                                                                               <  2:1367237/140‑1 (MQ=255)                                                         aaTACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCtgctgc                                                                              >  1:796907/1‑140 (MQ=255)                                                          aTACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTgctgct                                                                             <  2:358219/140‑1 (MQ=255)                                                           tACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCtt                                                                            >  2:409904/1‑140 (MQ=255)                                                            aCTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTc                                                                           <  2:957567/140‑1 (MQ=255)                                                            aCTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTc                                                                           <  2:129412/140‑1 (MQ=255)                                                             cTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCa                                                                          <  2:796907/140‑1 (MQ=255)                                                                gCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCTTAc                                                                       >  1:1620077/1‑140 (MQ=255)                                                                gCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATAc                                                                       >  1:718080/1‑140 (MQ=255)                                                                gCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATAc                                                                       <  1:1097151/140‑1 (MQ=255)                                                                gCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATAc                                                                       >  2:1097151/1‑140 (MQ=255)                                                                 ccATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCTAATGcctgcctg                                                                                  >  1:727555/1‑128 (MQ=255)                                                                 ccATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCTAATGcctgcctg                                                                                  <  2:727555/128‑1 (MQ=255)                                                                   aTCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGt                                                                    <  2:718080/140‑1 (MQ=255)                                                                   aTCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGt                                                                    <  1:1549088/140‑1 (MQ=255)                                                                   aTCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGt                                                                    <  2:271034/140‑1 (MQ=255)                                                                            cGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGc                                                           >  2:154637/1‑140 (MQ=255)                                                                            cGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGc                                                           >  2:1153897/1‑140 (MQ=255)                                                                                  aaCACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAgcg                                                     >  2:260067/1‑140 (MQ=255)                                                                                       gATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCAttt                                                <  2:496861/140‑1 (MQ=255)                                                                                            tCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAgcgc                                                    <  1:625779/131‑1 (MQ=255)                                                                                            tCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAgcgc                                                    >  2:625779/1‑131 (MQ=255)                                                                                             cGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAt                                          <  1:696470/140‑1 (MQ=255)                                                                                             cGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAt                                          <  1:572098/140‑1 (MQ=255)                                                                                              ggAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAtt                                         <  1:1153897/140‑1 (MQ=255)                                                                                                            cTCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATg                           <  2:908856/140‑1 (MQ=255)                                                                                                             tCCAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATgc                          <  2:1620077/140‑1 (MQ=255)                                                                                                              ccAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTa                                        >  2:1419894/1‑125 (MQ=255)                                                                                                              ccAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTa                                        <  1:1419894/125‑1 (MQ=255)                                                                                                               cAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATgcgc                        <  2:198808/140‑1 (MQ=255)                                                                                                               cAGCACCTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATgcgc                        >  2:1500513/1‑140 (MQ=255)                                                                                                                  cACCTGCTTCATTGCGATTGGCAACGCCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAt                      >  2:186504/1‑140 (MQ=255)                                                                                                                     cTGCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAttttt                  >  1:1554337/1‑140 (MQ=255)                                                                                                                       gCTTCATTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTAc                <  2:938884/140‑1 (MQ=255)                                                                                                                            aTTGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGtt           >  1:274891/1‑140 (MQ=255)                                                                                                                              tGCGATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTAt         <  2:274891/140‑1 (MQ=255)                                                                                                                               gCGATTGGCAACGCCCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTAt         <  1:186504/140‑1 (MQ=255)                                                                                                                                 gATTGGCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGa      <  1:154637/140‑1 (MQ=255)                                                                                                                                     ggCAACGC‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGt  >  1:577845/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                      CGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACG‑‑CCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGT  >  NC_000913/3332536‑3332806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑