Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,327,848	(C)5→6	coding (714/2229 nt)	crfC →	clamp‑binding sister replication fork colocalization protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,327,843	1	.	C	100.0%	254.7 / NA	72	T237P (ACC→CCC)	crfC	clamp‑binding sister replication fork colocalization protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (37/35);  total (37/35)

CGACTGGCGAAGGCGCTGGACGTCGATTTTCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATA‑CCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCG  >  NC_000913/4327708‑4327973                                                                                                                                         |                                                                                                                                   cGACTGGCGAAGGCGCTGGACGTCGATTTTCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATA‑cccc                                                                                                                                <  2:677864/140‑1 (MQ=255)    cTGGCGAAGGCGCTGGACGTCGATTTTCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATA‑ccccccg                                                                                                                             >  1:681599/1‑138 (MQ=255)    cTGGCGAAGGCGCTGGACGTCGATTTTCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATA‑ccccccg                                                                                                                             >  2:1653428/1‑138 (MQ=255)      ggCGAAGGCGCTGGACGTCGATTTTCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTTAGTTGACGTTACTGGATA‑ccccccggg                                                                                                                           <  1:383656/140‑5 (MQ=255)       gCGAAGGCGCTGGACGTCGATTTTCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATA‑ccccccgggc                                                                                                                          <  1:1271623/140‑6 (MQ=255)           aGGCGCTGGACGTCGATTTTCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCaaa                                                                                                                       <  1:1623187/140‑1 (MQ=255)              cgcTGGACGTCGATTTTCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGa                                                                                                                    >  2:1611044/1‑140 (MQ=255)                cTGGACGTCGATTTTCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGTGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAg                                                                                                                  >  1:1507443/1‑140 (MQ=255)                cTGGACGTCGATTTTCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAg                                                                                                                  <  1:555872/140‑1 (MQ=255)                cTGGACGTCGATTTTCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAg                                                                                                                  <  1:1595834/140‑1 (MQ=255)                                    ttCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCaa                                                                                              >  2:302327/1‑140 (MQ=255)                                      cTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCaaaa                                                                                            <  1:1653428/140‑1 (MQ=255)                                         cTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCaaaa                                                                                            >  2:1325463/1‑137 (MQ=255)                                         cTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCaaaa                                                                                            <  1:1325463/137‑1 (MQ=255)                                         cTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAAt                                                                                         >  2:137585/1‑140 (MQ=255)                                          ttACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATg                                                                                        >  1:15063/1‑140 (MQ=255)                                          ttACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGATCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATg                                                                                        >  1:442451/1‑140 (MQ=255)                                             cgccgcCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCtt                                                                                     >  1:1171228/1‑140 (MQ=255)                                                 gccATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAAcc                                                                                 >  2:413851/1‑140 (MQ=255)                                                  ccATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACca                                                                                <  2:1499412/140‑1 (MQ=255)                                                   cATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACcag                                                                               <  2:953716/140‑1 (MQ=255)                                                   cATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACcag                                                                               >  1:953716/1‑140 (MQ=255)                                                        aGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCt                                                                          >  1:37661/1‑140 (MQ=255)                                                          cATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTgg                                                                        >  1:1378408/1‑140 (MQ=255)                                                            tatTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCa                                                                      >  2:729709/1‑140 (MQ=255)                                                            tatTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCa                                                                      <  1:645602/140‑1 (MQ=255)                                                             atTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCa                                                                      <  2:329167/139‑1 (MQ=255)                                                             atTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCa                                                                      >  1:329167/1‑139 (MQ=255)                                                             atTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCAc                                                                     >  1:182846/1‑140 (MQ=255)                                                             atTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCAc                                                                     >  1:1612228/1‑140 (MQ=255)                                                             atTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGC‑TAACCAGCAGCTGGCAcg                                                                    >  1:1294644/1‑140 (MQ=255)                                                               tCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCAcgc                                                                   >  1:989852/1‑140 (MQ=255)                                                               tCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCAcgc                                                                   >  1:925049/1‑140 (MQ=255)                                                               tCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCAcg                                                                    >  2:1136840/1‑139 (MQ=255)                                                               tCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCAcg                                                                    <  1:1136840/139‑1 (MQ=255)                                                                 ccGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACgcgc                                                                 >  2:1071321/1‑140 (MQ=255)                                                                 ccGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACgcgc                                                                 <  1:940259/140‑1 (MQ=255)                                                                 ccGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACgcgc                                                                 <  1:137585/140‑1 (MQ=255)                                                                  cGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGcc                                                                >  2:1632319/1‑140 (MQ=255)                                                                   gTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGGAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCt                                                               >  2:1450910/1‑140 (MQ=255)                                                                    tGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTc                                                              >  2:321583/1‑140 (MQ=255)                                                                     gATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGC‑TAACCAGCAGCTGGCACGCGCCTcgg                                                            <  2:1294644/140‑1 (MQ=255)                                                                      aTTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTcgg                                                            <  1:302327/140‑1 (MQ=255)                                                                      aTTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTcgg                                                            >  1:298553/1‑140 (MQ=255)                                                                      aTTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTcgg                                                            <  2:895401/140‑1 (MQ=255)                                                                      aTTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTcgg                                                            >  2:96860/1‑140 (MQ=255)                                                                       ttGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTcggc                                                           <  2:1378408/140‑1 (MQ=255)                                                                       ttGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTcggc                                                           <  1:1138821/140‑1 (MQ=255)                                                                        tGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTcggcg                                                          >  1:571461/1‑140 (MQ=255)                                                                            gTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTAc                                                      >  2:1157858/1‑140 (MQ=255)                                                                               gAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTgg                                                   <  1:698681/140‑1 (MQ=255)                                                                               gAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTgg                                                   <  2:571461/140‑1 (MQ=255)                                                                               gAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTgg                                                   <  1:229826/140‑1 (MQ=255)                                                                                aGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGc                                                  >  1:888501/1‑140 (MQ=255)                                                                                 gTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGATCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCg                                                 <  2:442451/140‑1 (MQ=255)                                                                                    tGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTg                                              <  1:1450910/140‑1 (MQ=255)                                                                                    tGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTg                                              <  2:182846/140‑1 (MQ=255)                                                                                      tCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCt                                            <  1:96860/140‑1 (MQ=255)                                                                                         aTCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGa                                         <  1:321583/140‑1 (MQ=255)                                                                                           cTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGAtt                                       >  1:1061685/1‑140 (MQ=255)                                                                                           cTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGAtt                                       >  2:661922/1‑140 (MQ=255)                                                                                                ggggCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACg                                  <  2:1171228/140‑1 (MQ=255)                                                                                                ggggCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGGGGTTGAtttttat                                  >  2:1548420/1‑135 (MQ=255)                                                                                                         gagTTATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGaaa                         <  2:1061685/140‑1 (MQ=255)                                                                                                            ttATCCCGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCg                      <  1:1611044/140‑1 (MQ=255)                                                                                                                cccGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGAtct                  <  1:101634/140‑1 (MQ=255)                                                                                                                 ccGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGAtctc                 <  1:459356/140‑1 (MQ=255)                                                                                                                 ccGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGAtctc                 <  1:191299/140‑1 (MQ=255)                                                                                                                 ccGGTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGAtctc                 <  1:604276/140‑1 (MQ=255)                                                                                                                    gTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGGTTGGATATACACAAATTAAAAAGATCTCCGa              >  2:459356/1‑140 (MQ=255)                                                                                                                    gTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGGGGTGGGTTATTAGCCACTGAAAAAGATCTcccc              >  2:432930/1‑138 (MQ=255)                                                                                                                    gTCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGGGCTGGGTTTTAAGCCACTGGAAAAGATCTccca              >  1:1010886/1‑138 (MQ=255)                                                                                                                     tCAGTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGAt             <  2:202093/140‑1 (MQ=255)                                                                                                                        gTTGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGaa          <  1:1157858/140‑1 (MQ=255)                                                                                                                         ttGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGGGGTGGGGTATAAGCAAACGAAAAACATCTCCCACGaaa         >  2:191299/1‑139 (MQ=255)                                                                                                                         ttGACGTTACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGGGCTGGGGTATAAGCCACAGGAAAACATCTCCGCTGAag         >  1:215069/1‑140 (MQ=255)                                                                                                                                tACTGGATACCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCg  <  1:682413/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   CGACTGGCGAAGGCGCTGGACGTCGATTTTCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATA‑CCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCG  >  NC_000913/4327708‑4327973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑