Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	411,056	(A)6→7	intergenic (+4/+241)	mak → / ← araJ	manno(fructo)kinase/putative transport protein AraJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	411,050	1	.	A	100.0%	161.9 / NA	46	*303K (TAA→AAA)	mak	manno(fructo)kinase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (20/26);  total (20/26)

AGACCGTTTATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGT‑AAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTGCTCCGATGCCT  >  NC_000913/410914‑411183                                                                                                                                          |                                                                                                                                      aGACCGTTTATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCATAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGT‑aaa                                                                                                                                    <  1:894815/140‑1 (MQ=255)   aCCGTTTATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGT‑aaaaa                                                                                                                                  >  1:1285453/1‑140 (MQ=255)       tttATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGT‑aaaaaaacg                                                                                                                              >  2:1359891/1‑137 (MQ=255)        ttATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGT‑aaaaaaacgt                                                                                                                             >  2:1330526/1‑136 (MQ=255)            atCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGc                                                                                                                          >  2:191543/1‑140 (MQ=255)                 aCGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTg                                                                                                                     >  2:129725/1‑140 (MQ=255)                 aCGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTg                                                                                                                     <  1:767185/140‑1 (MQ=255)                  cGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTgg                                                                                                                    <  2:1075565/140‑1 (MQ=255)                  cGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTgg                                                                                                                    <  1:1359891/140‑1 (MQ=255)                  cGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTgg                                                                                                                    <  1:1660549/140‑1 (MQ=255)                       gggCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGcat                                                                                                               >  2:851627/1‑140 (MQ=255)                                   aaaCAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGc                                                                                                   <  2:1489171/140‑1 (MQ=255)                                   aaaCAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGc                                                                                                   <  1:191543/140‑1 (MQ=255)                                       aaTTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACg                                                                                               >  2:1180994/1‑140 (MQ=255)                                         tttGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGcatc                                                                                                              >  2:1242573/1‑123 (MQ=255)                                         tttGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGcatc                                                                                                              <  1:1242573/123‑1 (MQ=255)                                            gTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGc                                                                                          <  1:337506/140‑1 (MQ=255)                                            gTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGc                                                                                          <  2:430259/140‑1 (MQ=255)                                             tCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGcc                                                                                         <  1:498572/140‑1 (MQ=255)                                             tCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGcc                                                                                         <  1:1206141/140‑1 (MQ=255)                                                       cgAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                               <  2:1376187/140‑1 (MQ=255)                                                       cgAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                               <  2:356046/140‑1 (MQ=255)                                                         aaTGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                             <  2:1285453/140‑1 (MQ=255)                                                               aaaCGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc                                                                       >  1:212217/1‑140 (MQ=255)                                                                 aCGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACaa                                                                     >  2:1273924/1‑140 (MQ=255)                                                                   gCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACaaaa                                                                   <  1:1180994/140‑1 (MQ=255)                                                                     cGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAgg                                                                 >  1:1389026/1‑140 (MQ=255)                                                                     cGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAgg                                                                 >  1:352327/1‑140 (MQ=255)                                                                       gTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTg                                                               >  2:832895/1‑140 (MQ=255)                                                                          cGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCa                                                            <  1:447032/140‑1 (MQ=255)                                                                               ggCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAAcc                                                       <  1:851627/140‑1 (MQ=255)                                                                                gCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCg                                                      <  1:1273924/140‑1 (MQ=255)                                                                                gCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCg                                                      <  2:1404415/140‑1 (MQ=255)                                                                                   aaGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCGTAg                                                   <  1:464419/140‑1 (MQ=255)                                                                                    aGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCACATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCGTAgg                                                  >  1:1160606/1‑140 (MQ=255)                                                                                          gTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCGTAGGCCGGAt                                            >  1:1197216/1‑140 (MQ=255)                                                                                              ttCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCGTAGGCCGGATAAgg                                        >  2:1663974/1‑140 (MQ=255)                                                                                              ttCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCGTAGGCCGGATAAgg                                        <  2:221412/140‑1 (MQ=255)                                                                                                     ggcgTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCGTAGGCCGGATAAGGCGTTCAc                                 >  1:22782/1‑140 (MQ=255)                                                                                                       cgTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCGTAGGCCGGATAAGGCGTTCAcgc                               >  2:21232/1‑140 (MQ=255)                                                                                                         tACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCGTAGGCCGGATAAGGCGTTCAcgccg                             <  1:316854/140‑1 (MQ=255)                                                                                                            gcggcgCTGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCGTAGGCCGGATAAGGCGTTCACGCCGCAt                          <  1:946744/140‑1 (MQ=255)                                                                                                                   tGCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                   >  2:769072/1‑140 (MQ=255)                                                                                                                    gCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAt                  <  1:832895/140‑1 (MQ=255)                                                                                                                    gCGTGGTTATGGCCACAAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCGTAGGCCGGATAAGGCGTACACGCCGCGTCCGGCAAt                  <  2:352327/140‑1 (MQ=255)                                                                                                                                 cacaAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAt                  >  2:1155558/1‑127 (MQ=255)                                                                                                                                 cacaAGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAt                  <  1:1155558/127‑1 (MQ=255)                                                                                                                                    aaGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTGCTCCGATGCCt  >  1:398633/1‑140 (MQ=255)                                                                                                                                    aaGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTGCTCCGATGCCt  >  1:554179/1‑140 (MQ=255)                                                                                                                                    aaGAGTAAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTGCTCCGATGCCt  >  1:1644474/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      AGACCGTTTATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGT‑AAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTGCTCCGATGCCT  >  NC_000913/410914‑411183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑