BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,908,667	(A)_5→6	coding (100/144 nt)	mgrB ←	PhoQ kinase inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,908,662	1	.	A	98.4%	455.2 / ‑1.6	128	coding (105/144 nt)	mgrB	PhoQ kinase inhibitor
Reads supporting (aligned to +/- strand): ref base . (1/1); new base A (67/59); total (68/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

Only 100 of 133 total aligned reads displayed.

GCCTGAATTTACAACTTCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑G‑AAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCA  >  NC_000913/1908525‑1908796

gCCTGAATTTACAACTTCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑G‑aa                                                                                                                                      >  2:1303742/1‑140 (MQ=255)

gCCTGAATTTACAACTTCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑G‑aa                                                                                                                                      >  1:158043/1‑140 (MQ=255)

  cTGAATTTACAACTTCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑G‑aaaa                                                                                                                                    >  1:1156921/1‑140 (MQ=255)

          aCAACTTCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTAc                                                                                                                             >  2:1067391/1‑140 (MQ=255)

             aCTTCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATc                                                                                                                          <  1:1155131/140‑1 (MQ=255)

              cTTCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATcc                                                                                                                         <  2:458088/140‑1 (MQ=255)

               ttCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCt                                                                                                                        <  1:1018948/140‑1 (MQ=255)

                tCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTg                                                                                                                       >  1:1413772/1‑140 (MQ=255)

                   ttCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATc                                                                                                                    >  2:368275/1‑140 (MQ=255)

                       tttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGcac                                                                                                                <  2:1567672/140‑1 (MQ=255)

                         tCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCAcat                                                                                                              <  1:1224555/140‑1 (MQ=255)

                          caccacCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCAcatc                                                                                                             <  2:1436333/140‑1 (MQ=255)

                          caccacCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCAcatc                                                                                                             <  1:1303742/140‑1 (MQ=255)

                          caccacCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCAcatc                                                                                                             <  1:893141/140‑1 (MQ=255)

                           accaccTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCAcatca                                                                                                            <  2:870314/140‑1 (MQ=255)

                             caccTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGcaca                                                                                                               >  2:1326271/1‑135 (MQ=255)

                                     aGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATATAGTGCTGAAAAAATGATAGCACCACGGGATAAACTGATTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATAc                                                                                                  >  2:1488365/1‑140 (MQ=255)

                                          agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTgcg                                                                                             >  2:1119599/1‑140 (MQ=255)

                                          agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTgcg                                                                                             >  1:156384/1‑140 (MQ=255)

                                          agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTgcg                                                                                             >  1:1555357/1‑140 (MQ=255)

                                          agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTgcg                                                                                             >  2:571371/1‑140 (MQ=255)

                                            gcgcTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCAcatc                                                                                                             <  1:1621378/122‑1 (MQ=255)

                                            gcgcTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCAcatc                                                                                                             >  2:1621378/1‑122 (MQ=255)

                                            gcgcTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑CAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGcc                                                                                           <  2:878871/140‑1 (MQ=255)

                                               cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATg                                                                                                          <  1:1083619/122‑1 (MQ=255)

                                                 aTTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAag                                                                                      >  2:1159114/1‑140 (MQ=255)

                                                   tCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAACGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAagca                                                                                    <  2:1555357/140‑1 (MQ=255)

                                                        cACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaag                                                                               <  2:847622/140‑1 (MQ=255)

                                                          ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                             >  1:905585/1‑140 (MQ=255)

                                                          ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                             >  2:1457479/1‑140 (MQ=255)

                                                            gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                           >  2:150961/1‑140 (MQ=255)

                                                            gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                           >  1:1402536/1‑140 (MQ=255)

                                                            gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                           >  1:199295/1‑140 (MQ=255)

                                                            gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                           >  1:1356597/1‑140 (MQ=255)

                                                              tgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGcc                                                                         <  1:53516/140‑1 (MQ=255)

                                                                gagaGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCaa                                                                       <  2:199295/140‑1 (MQ=255)

                                                                gagaGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCaa                                                                       <  1:736698/140‑1 (MQ=255)

                                                                gagaGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAACAAGCCaa                                                                       <  2:1356597/140‑1 (MQ=255)

                                                                 agagGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAac                                                                      >  2:259697/1‑140 (MQ=255)

                                                                     gaagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAaca                                                                     <  1:133044/137‑1 (MQ=255)

                                                                     gaagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAaca                                                                     >  2:133044/1‑137 (MQ=255)

                                                                     gaagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAAcacca                                                                  >  2:727636/1‑140 (MQ=255)

                                                                       agaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaag                                                                               >  2:1384930/1‑125 (MQ=255)

                                                                       agaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACg                                                                >  1:171721/1‑140 (MQ=255)

                                                                       agaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACg                                                                >  2:515271/1‑140 (MQ=255)

                                                                          aaaTCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACa                                                             >  1:1508518/1‑140 (MQ=255)

                                                                           aaTCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACaa                                                            >  2:849228/1‑140 (MQ=255)

                                                                            aTCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAAc                                                           <  1:727636/140‑1 (MQ=255)

                                                                               tAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAg                                                        >  2:41108/1‑140 (MQ=255)

                                                                                aGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGa                                                       >  2:498486/1‑140 (MQ=255)

                                                                                  tGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAAc                                                     >  1:613540/1‑140 (MQ=255)

                                                                                   gCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGTACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACg                                                    >  1:732017/1‑140 (MQ=255)

                                                                                     tGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGAc                                                  <  1:849228/140‑1 (MQ=255)

                                                                                       aaaaaaTGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACGACCAGAACGAccc                                                >  2:280177/1‑140 (MQ=255)

                                                                                         aaaaTGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCAt                                              <  1:862841/140‑1 (MQ=255)

                                                                                          aaaTGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATc                                             <  2:764705/140‑1 (MQ=255)

                                                                                           aaTGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCg                                            >  1:150117/1‑140 (MQ=255)

                                                                                                 atCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACtt                                      >  2:224305/1‑140 (MQ=255)

                                                                                                   caccacGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACtttt                                    >  1:663744/1‑140 (MQ=255)

                                                                                                    accacGGGATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACttttt                                   >  1:8/1‑140 (MQ=255)

                                                                                                           gATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGaaa                                         >  2:698034/1‑127 (MQ=255)

                                                                                                           gATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGaaa                                         <  1:698034/127‑1 (MQ=255)

                                                                                                           gATAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCa                            <  1:498486/140‑1 (MQ=255)

                                                                                                             tAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAt                          <  1:1067391/140‑1 (MQ=255)

                                                                                                             tAAACTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAt                          >  2:734051/1‑140 (MQ=255)

                                                                                                              aaaCTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAtc                         <  2:1606549/140‑1 (MQ=255)

                                                                                                                aCTGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAtctc                       >  1:638672/1‑140 (MQ=255)

                                                                                                                  tGGTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCg                     <  1:41108/140‑1 (MQ=255)

                                                                                                                   ggTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGt                    <  2:638672/140‑1 (MQ=255)

                                                                                                                   ggTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGt                    >  1:854052/1‑140 (MQ=255)

                                                                                                                   ggTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGt                    >  1:348526/1‑140 (MQ=255)

                                                                                                                    gTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGt                    >  1:759106/1‑139 (MQ=255)

                                                                                                                    gTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGt                    <  2:759106/139‑1 (MQ=255)

                                                                                                                    gTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTGTCACTCCAATCTCCGtt                   >  2:1482082/1‑140 (MQ=255)

                                                                                                                    gTTAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCACCGAAACTTTTTCACTCCAATCTCCGtt                   >  2:1013771/1‑140 (MQ=255)

                                                                                                                     ttAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGttt                  >  2:1098779/1‑140 (MQ=255)

                                                                                                                     ttAATGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGttt                  >  1:1257493/1‑140 (MQ=255)

                                                                                                                       aaTGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCa                            >  2:1142822/1‑128 (MQ=255)

                                                                                                                       aaTGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCa                            <  1:1142822/128‑1 (MQ=255)

                                                                                                                        aTGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac               <  1:1574590/140‑1 (MQ=255)

                                                                                                                        aTGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac               <  1:1582448/140‑1 (MQ=255)

                                                                                                                        aTGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac               <  1:1329384/140‑1 (MQ=255)

                                                                                                                         tGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGtt                   >  1:1531424/1‑135 (MQ=255)

                                                                                                                         tGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGtt                   <  2:1531424/135‑1 (MQ=255)

                                                                                                                         tGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacc              >  1:990203/1‑140 (MQ=255)

                                                                                                                         tGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac               <  1:817679/139‑1 (MQ=255)

                                                                                                                         tGGCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac               >  2:817679/1‑139 (MQ=255)

                                                                                                                          ggCACAAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacct             >  2:1420280/1‑140 (MQ=255)

                                                                                                                            cacaAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGt                    >  1:1032952/1‑131 (MQ=255)

                                                                                                                            cacaAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGt                    <  2:1032952/131‑1 (MQ=255)

                                                                                                                              caAATTCCGCTAA‑AAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCa                <  1:816792/133‑1 (MQ=255)

                                                                                                                              caAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacctacct         <  2:663744/140‑1 (MQ=255)

                                                                                                                              caAATTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacctacct         >  1:774905/1‑140 (MQ=255)

                                                                                                                                aaTTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTa       <  1:150961/140‑1 (MQ=255)

                                                                                                                                 aTTCCGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTAt      <  2:905585/140‑1 (MQ=255)

                                                                                                                                     cGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCa  <  2:348526/140‑1 (MQ=255)

                                                                                                                                     cGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCa  <  2:774905/140‑1 (MQ=255)

                                                                                                                                     cGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCa  <  1:515271/140‑1 (MQ=255)

                                                                                                                                     cGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCa  <  1:445300/140‑1 (MQ=255)

                                                                                                                                     cGCT‑GAAAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCa  <  1:1420280/140‑1 (MQ=255)

GCCTGAATTTACAACTTCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCT‑G‑AAAAATTGTACATCCTGATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCA  >  NC_000913/1908525‑1908796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`