Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,237,763	+C	intergenic (‑10/+6)	mglC ← / ← mglA	D‑galactose/methyl‑galactoside ABC transporter membrane subunit/D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,237,759	1	.	C	100.0%	283.0 / NA	79	intergenic (‑6/+10)	mglC/mglA	D‑galactose/methyl‑galactoside ABC transporter membrane subunit/D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (36/43);  total (36/43)

TTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAG‑CCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGG  >  NC_000913/2237623‑2237891                                                                                                                                          |                                                                                                                                     ttACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAG‑ccc                                                                                                                                   >  1:902144/1‑140 (MQ=255) ttACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAG‑ccc                                                                                                                                   <  1:76227/140‑1 (MQ=255)  tACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAGTAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAG‑cccc                                                                                                                                  >  2:300629/1‑140 (MQ=255)  tACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAG‑cccc                                                                                                                                  <  1:708221/140‑1 (MQ=255)    cTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAG‑ccccct                                                                                                                                <  2:1107987/140‑3 (MQ=255)    cTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAG‑ccccct                                                                                                                                <  1:1149300/140‑3 (MQ=255)    cTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAG‑ccccct                                                                                                                                <  2:996266/140‑3 (MQ=255)       aaGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAATTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAG‑ccccctaat                                                                                                                             >  1:997650/1‑135 (MQ=255)       aaGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAG‑ccccctaat                                                                                                                             >  1:766321/1‑135 (MQ=255)         gTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCt                                                                                                                            >  2:655520/1‑140 (MQ=255)         gTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCt                                                                                                                            >  1:631477/1‑140 (MQ=255)          ttCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCtt                                                                                                                           <  2:452365/140‑1 (MQ=255)          ttCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCtt                                                                                                                           >  2:243542/1‑140 (MQ=255)          ttCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCtt                                                                                                                           >  2:1425492/1‑140 (MQ=255)            cAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGGAGCCCCCTAATCTTaa                                                                                                                         <  1:113403/140‑1 (MQ=255)            cAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTaa                                                                                                                         >  2:862271/1‑140 (MQ=255)            cAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTaa                                                                                                                         >  1:1063971/1‑140 (MQ=255)             aaCAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAG‑cccc                                                                                                                                  >  1:999524/1‑129 (MQ=255)             aaCAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAG‑cccc                                                                                                                                  <  2:999524/129‑1 (MQ=255)               cAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAgg                                                                                                                      >  2:1637599/1‑140 (MQ=255)                  aCTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGc                                                                                                                   >  1:1268891/1‑140 (MQ=255)                  aCTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAG‑ccccct                                                                                                                                <  2:1423980/126‑3 (MQ=255)                  aCTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAG‑ccccct                                                                                                                                >  1:1423980/1‑124 (MQ=255)                   cTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCa                                                                                                                  >  1:1301148/1‑140 (MQ=255)                    ttAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCaa                                                                                                                 <  1:243542/140‑1 (MQ=255)                      aaaaaTGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAaag                                                                                                               <  2:1341925/140‑1 (MQ=255)                      aaaaaTGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAaag                                                                                                               <  2:1622368/140‑1 (MQ=255)                           tGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAaagacgca                                                                                                          <  1:950544/140‑1 (MQ=255)                           tGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAaagacgca                                                                                                          <  1:961490/140‑1 (MQ=255)                            gTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAaagacgcaa                                                                                                         >  1:35317/1‑140 (MQ=255)                                ggTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAaagacgcaagacg                                                                                                     >  1:1646431/1‑140 (MQ=255)                                  tCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAaagacgcaagacgta                                                                                                   <  1:622135/140‑3 (MQ=255)                                     tGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGaa                                                                                                >  1:863513/1‑140 (MQ=255)                                            taataatCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGtt                                                                                         >  2:1081664/1‑140 (MQ=255)                                                aatCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGc                                                                                     <  2:1230107/140‑1 (MQ=255)                                                aatCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGc                                                                                     <  2:790877/140‑1 (MQ=255)                                                 atCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCg                                                                                    >  1:356509/1‑140 (MQ=255)                                                     ccAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgt                                                                                <  1:862271/140‑1 (MQ=255)                                                      cAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgtt                                                                               >  2:1335687/1‑140 (MQ=255)                                                         cAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgttgtt                                                                            >  1:1487709/1‑140 (MQ=255)                                                          aaaaTTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGttt                                                                           <  2:997650/140‑1 (MQ=255)                                                            aaCTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGttttt                                                                         <  2:356509/140‑1 (MQ=255)                                                              cTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgt                                                                                   <  1:935898/128‑1 (MQ=255)                                                              cTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgt                                                                                   >  2:935898/1‑128 (MQ=255)                                                              cTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGt                                                                       <  2:35317/140‑1 (MQ=255)                                                                       cgacgTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAAt                                                              >  2:572364/1‑140 (MQ=255)                                                                        gacgTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAAtt                                                             >  1:1131345/1‑140 (MQ=255)                                                                           gTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGAc                                                                 >  1:614808/1‑133 (MQ=255)                                                                           gTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGAc                                                                 <  2:614808/133‑1 (MQ=255)                                                                           gTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCg                                                          >  1:864987/1‑140 (MQ=255)                                                                             aaaTACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGa                                                        <  2:902144/140‑1 (MQ=255)                                                                                    gCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACgaga                                                 >  1:198048/1‑140 (MQ=255)                                                                                    gCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACgaga                                                 >  2:631351/1‑140 (MQ=255)                                                                                     cccTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGAc                                                >  2:854452/1‑140 (MQ=255)                                                                                       ctctTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCa                                              <  1:300629/140‑1 (MQ=255)                                                                                       ctctTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCa                                              <  1:1425492/140‑1 (MQ=255)                                                                                        tctTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCAt                                             <  2:1070922/140‑1 (MQ=255)                                                                                        tctTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCAt                                             <  2:208187/140‑1 (MQ=255)                                                                                               ggTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCAt                                      >  2:75075/1‑140 (MQ=255)                                                                                               ggTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCAt                                      <  1:1335687/140‑1 (MQ=255)                                                                                                 taagtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGa                                    <  2:1063971/140‑1 (MQ=255)                                                                                                 taagtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGa                                    <  2:631477/140‑1 (MQ=255)                                                                                                  aagtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGAc                                   <  1:1081664/140‑1 (MQ=255)                                                                                                  aagtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGAc                                   <  1:1429976/140‑1 (MQ=255)                                                                                                  aagtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGAc                                   <  2:1646431/140‑1 (MQ=255)                                                                                                  aagtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGAc                                   <  1:75075/140‑1 (MQ=255)                                                                                                    gtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCa                                 >  1:1127517/1‑140 (MQ=255)                                                                                                    gtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCa                                 >  2:1061199/1‑140 (MQ=255)                                                                                                    gtaagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCa                                 >  1:1135842/1‑140 (MQ=255)                                                                                                     taagAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAg                                >  1:1157924/1‑140 (MQ=255)                                                                                                           aaCTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACg                          <  1:572364/140‑1 (MQ=255)                                                                                                           aaCTTTTCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACg                          <  2:1135842/140‑1 (MQ=255)                                                                                                               tttCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCAt                                      >  2:191943/1‑124 (MQ=255)                                                                                                               tttCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCAt                                      <  1:191943/124‑1 (MQ=255)                                                                                                               tttCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGAc                                   <  2:61080/127‑1 (MQ=255)                                                                                                               tttCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGAc                                   >  1:61080/1‑127 (MQ=255)                                                                                                               tttCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCt                      >  1:402380/1‑140 (MQ=255)                                                                                                                ttCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTg                     <  2:1449651/140‑1 (MQ=255)                                                                                                                ttCTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTg                     <  2:864987/140‑1 (MQ=255)                                                                                                                  cTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTg                     >  1:22553/1‑138 (MQ=255)                                                                                                                  cTTATTTAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTg                     <  2:22553/138‑1 (MQ=255)                                                                                                                        tAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAAt                             <  2:1434472/124‑1 (MQ=255)                                                                                                                        tAACGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAAt                             >  1:1434472/1‑124 (MQ=255)                                                                                                                           cGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTaac          >  2:485821/1‑140 (MQ=255)                                                                                                                           cGCACTCATGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTaac          >  1:410294/1‑140 (MQ=255)                                                                                                                                   tGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAgg  <  1:485821/140‑1 (MQ=255)                                                                                                                                   tGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAgg  <  2:1157924/140‑1 (MQ=255)                                                                                                                                   tGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAgg  <  1:655520/140‑1 (MQ=255)                                                                                                                                   tGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAgg  <  1:1637599/140‑1 (MQ=255)                                                                                                                                   tGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAgg  <  1:631351/140‑1 (MQ=255)                                                                                                                                   tGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAgg  <  2:1487709/140‑1 (MQ=255)                                                                                                                                   tGGGAAGCCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTCGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAgg  <  1:738897/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     TTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAG‑CCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGG  >  NC_000913/2237623‑2237891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑