Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	10,265	A→G	L77P (CTG→CCG)	satP ←	acetate/succinate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	10,265	0	A	G	100.0%	311.5 / NA	88	L77P (CTG→CCG)	satP	acetate/succinate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/42);  total (46/42)

CGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAA  >  NC_000913/10128‑10401                                                                                                                                          |                                                                                                                                         cgcgTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGc                                                                                                                                        <  1:59530/140‑1 (MQ=255)   cgTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCa                                                                                                                                      >  1:59317/1‑140 (MQ=255)    gTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCag                                                                                                                                     >  1:1345258/1‑140 (MQ=255)     tGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCaga                                                                                                                                    >  2:1310611/1‑140 (MQ=255)      gcgcCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCagaa                                                                                                                                   <  1:264029/140‑1 (MQ=255)           tttCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAAc                                                                                                                              >  1:211385/1‑140 (MQ=255)                   tGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAagag                                                                                                                      <  2:1010932/140‑1 (MQ=255)                    gCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGt                                                                                                                           <  1:469983/134‑1 (MQ=255)                    gCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGt                                                                                                                           >  2:469983/1‑134 (MQ=255)                    gCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAgg                                                                                                                     >  2:480896/1‑140 (MQ=255)                      cGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGGGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCagaaaga                                                                                                                                >  2:311710/1‑127 (MQ=255)                      cGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGGGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCagaaaga                                                                                                                                <  1:311710/127‑1 (MQ=255)                      cGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTg                                                                                                                   >  2:1650580/1‑140 (MQ=255)                          gaaCATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATg                                                                                                               <  1:1650580/140‑1 (MQ=255)                              aTAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGt                                                                                                           <  1:1163278/140‑1 (MQ=255)                                aaaCAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTa                                                                                                         <  2:1345258/140‑1 (MQ=255)                                 aaCAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTaa                                                                                                        <  1:1310611/140‑1 (MQ=255)                                     gCGTAAATACGCCCCACAGACCCAGGTAGACACCGAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCg                                                                                                    >  2:972408/1‑140 (MQ=255)                                     gCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCg                                                                                                    >  2:356996/1‑140 (MQ=255)                                     gCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCg                                                                                                    >  1:1573926/1‑140 (MQ=255)                                     gCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCg                                                                                                    >  2:1042574/1‑140 (MQ=255)                                     gCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCg                                                                                                    >  2:621902/1‑140 (MQ=255)                                     gCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCg                                                                                                    >  1:369611/1‑140 (MQ=255)                                      cGTAAATACGCCCCACAGATCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGa                                                                                                   >  1:1188070/1‑140 (MQ=255)                                      cGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGGACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGa                                                                                                   >  1:134559/1‑140 (MQ=255)                                      cGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCTGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGa                                                                                                   >  1:859921/1‑140 (MQ=255)                                       gTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAGCCGTAAGAGGTGAATGCGGTTAAACCGaa                                                                                                  >  2:1568353/1‑140 (MQ=255)                                       gTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGaa                                                                                                  >  1:317962/1‑140 (MQ=255)                                       gTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGaa                                                                                                  >  2:1031041/1‑140 (MQ=255)                                              cGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTg                                                                                           >  2:1527239/1‑140 (MQ=255)                                               gCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGATTCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGc                                                                                          <  2:128205/140‑1 (MQ=255)                                                 cccACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCt                                                                                        <  2:435024/140‑1 (MQ=255)                                                  ccACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCtt                                                                                       >  1:1302577/1‑140 (MQ=255)                                                    acaGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCtttt                                                                                     >  1:491522/1‑140 (MQ=255)                                                    acaGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCtttt                                                                                     <  2:432054/140‑1 (MQ=255)                                                     caGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCttttt                                                                                    >  2:310064/1‑140 (MQ=255)                                                     caGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCttttt                                                                                    >  2:322988/1‑140 (MQ=255)                                                     caGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCttttt                                                                                    <  2:1573926/140‑1 (MQ=255)                                                     caGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCttttt                                                                                    <  2:369611/140‑1 (MQ=255)                                                       gACCCAGGTAGACACCAAGGGACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTg                                                                                  <  2:134559/140‑1 (MQ=255)                                                       gACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTg                                                                                  <  2:211385/140‑1 (MQ=255)                                                        aCCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGt                                                                                 >  2:930864/1‑140 (MQ=255)                                                         cccAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGtt                                                                                            >  1:604048/1‑128 (MQ=255)                                                         cccAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGtt                                                                                            <  2:604048/128‑1 (MQ=255)                                                         cccAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTa                                                                                <  2:317962/140‑1 (MQ=255)                                                         cccAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTa                                                                                <  1:862498/140‑1 (MQ=255)                                                         cccAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTa                                                                                <  1:562130/140‑1 (MQ=255)                                                          ccAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAgtgt                                                                                             >  1:524237/1‑126 (MQ=255)                                                          ccAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAgtgt                                                                                             <  2:524237/126‑1 (MQ=255)                                                            aGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTc                                                                             >  2:787914/1‑140 (MQ=255)                                                                  acacCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCcagcag                                                                       >  2:114210/1‑140 (MQ=255)                                                                    acCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGAc                                                                     >  1:519534/1‑140 (MQ=255)                                                                    acCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGAc                                                                     <  1:621902/140‑1 (MQ=255)                                                                       aaGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAg                                                                  >  2:1418958/1‑140 (MQ=255)                                                                             cTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCaaaaa                                                            >  1:1290352/1‑140 (MQ=255)                                                                                tgCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACTGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAAttt                                                         >  1:1532662/1‑140 (MQ=255)                                                                                tgCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAAttt                                                         >  1:1321144/1‑140 (MQ=255)                                                                                   aTTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTgcg                                                      <  1:1031041/140‑1 (MQ=255)                                                                                       ggCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGAt                                                  >  1:299300/1‑140 (MQ=255)                                                                                        gcgcATCTGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCACACCAGCAAAAATTTGCGCGATg                                                 <  2:859921/140‑1 (MQ=255)                                                                                        gcgcATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATg                                                 <  1:144392/140‑1 (MQ=255)                                                                                         cgcATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATgc                                                <  1:787914/140‑1 (MQ=255)                                                                                          gcATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATgcc                                               >  1:1504614/1‑140 (MQ=255)                                                                                           cATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATgccg                                              <  1:114210/140‑1 (MQ=255)                                                                                            aTCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTgcg                                                      >  2:1284438/1‑131 (MQ=255)                                                                                            aTCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTgcg                                                      <  1:1284438/131‑1 (MQ=255)                                                                                            aTCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATgccgc                                             <  1:480896/140‑1 (MQ=255)                                                                                             tCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATgccgcc                                            <  1:322988/140‑1 (MQ=255)                                                                                                    gACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGaaa                                     <  1:930864/140‑1 (MQ=255)                                                                                                          gTTTCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGccc                               <  2:59317/140‑1 (MQ=255)                                                                                                           tttCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCa                              >  2:817797/1‑140 (MQ=255)                                                                                                           tttCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCa                              >  2:39059/1‑140 (MQ=255)                                                                                                             tCGGCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATg                            <  1:356996/140‑1 (MQ=255)                                                                                                               ggCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGc                          <  1:310064/140‑1 (MQ=255)                                                                                                               ggCATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGc                          <  1:812518/140‑1 (MQ=255)                                                                                                               ggCACCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACTGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGc                          <  2:1532662/140‑1 (MQ=255)                                                                                                                 cATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGc                          <  1:633858/138‑1 (MQ=255)                                                                                                                 cATCAGCAGAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGc                          >  2:633858/1‑138 (MQ=255)                                                                                                                    cagcagAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGaa                     <  2:405366/140‑1 (MQ=255)                                                                                                                     agcagAATCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGaat                    >  2:1348977/1‑140 (MQ=255)                                                                                                                          aaTCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGc                          <  1:1090055/129‑1 (MQ=255)                                                                                                                          aaTCGCAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGc                          >  2:1090055/1‑129 (MQ=255)                                                                                                                               cAACCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTcc          >  1:309175/1‑140 (MQ=255)                                                                                                                                 aCCAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCag        >  1:1415457/1‑140 (MQ=255)                                                                                                                                   cAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCagag      <  2:519534/140‑1 (MQ=255)                                                                                                                                   cAGCGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCagag      <  1:1042574/140‑1 (MQ=255)                                                                                                                                      cGTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGa   >  1:37102/1‑140 (MQ=255)                                                                                                                                       gTCGGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGaa  <  2:1188070/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAA  >  NC_000913/10128‑10401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑