Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	245,217	G→A	Q197* (CAA→TAA)	yafK ←	L,D‑transpeptidase domain‑containing protein YafK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	245,217	0	G	A	100.0%	264.3 / NA	77	Q197* (CAA→TAA)	yafK	L,D‑transpeptidase domain‑containing protein YafK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/41);  total (36/41)

GAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCAAACACCAGCGCACCAGTAACGAACTGGAATATCTCATCA  >  NC_000913/245080‑245353                                                                                                                                          |                                                                                                                                         gAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACt                                                                                                                                        <  2:796688/140‑1 (MQ=255)    cGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTccc                                                                                                                                     >  2:216876/1‑140 (MQ=255)    cGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTccc                                                                                                                                     >  1:1200594/1‑140 (MQ=255)    cGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTccc                                                                                                                                     >  1:251120/1‑140 (MQ=255)       gtAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGa                                                                                                                                  <  1:1220125/140‑1 (MQ=255)       gtAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGa                                                                                                                                  <  2:1582615/140‑1 (MQ=255)       gtAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGa                                                                                                                                  <  2:251120/140‑1 (MQ=255)        tAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGaa                                                                                                                                 >  2:599787/1‑140 (MQ=255)        tAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGaa                                                                                                                                 <  1:841377/140‑1 (MQ=255)             tGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCt                                                                                                                            >  1:907386/1‑140 (MQ=255)               aTGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTa                                                                                                                          >  1:1647539/1‑140 (MQ=255)                 gCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCCCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTaaa                                                                                                                        >  1:1397900/1‑140 (MQ=255)                  ccAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAg                                                                                                                       <  2:1403770/140‑1 (MQ=255)                   cAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGt                                                                                                                      >  1:1409385/1‑140 (MQ=255)                      ttGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTgg                                                                                                                   <  2:907386/140‑1 (MQ=255)                          ggCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAata                                                                                                               >  2:1374256/1‑140 (MQ=255)                               cacaACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTAt                                                                                                          >  2:258017/1‑140 (MQ=255)                               cacaACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTAt                                                                                                          <  2:1200594/140‑1 (MQ=255)                                acaACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATg                                                                                                         >  2:388421/1‑140 (MQ=255)                                 caACTTCGTGGCTTAACGGCTTGCTGACCACGTCACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATgg                                                                                                        <  2:1324895/140‑1 (MQ=255)                                 caACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATgg                                                                                                        <  1:216876/140‑1 (MQ=255)                                   aCTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGCTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGgcg                                                                                                      <  1:324928/140‑1 (MQ=255)                                    cTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGgcgc                                                                                                     >  1:1011815/1‑140 (MQ=255)                                     ttCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCt                                                                                                    <  1:918192/140‑1 (MQ=255)                                     ttCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCt                                                                                                    <  1:966864/140‑1 (MQ=255)                                         tGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCat                                                                                                >  2:567602/1‑140 (MQ=255)                                         tGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCat                                                                                                >  2:1533095/1‑140 (MQ=255)                                          ggCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCata                                                                                               <  1:599787/140‑1 (MQ=255)                                          ggCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCata                                                                                               <  2:1011815/140‑1 (MQ=255)                                              tAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTgg                                                                                           >  1:254640/1‑140 (MQ=255)                                              tAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATCGCGCTTCATATTgg                                                                                           >  1:376880/1‑140 (MQ=255)                                                   gCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCg                                                                                      >  1:1428982/1‑140 (MQ=255)                                                         tGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATg                                                                                <  2:254640/140‑1 (MQ=255)                                                          gACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGc                                                                               >  2:1127765/1‑140 (MQ=255)                                                            ccACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCgg                                                                             >  2:57613/1‑140 (MQ=255)                                                              aCGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGaa                                                                           <  1:388421/140‑1 (MQ=255)                                                              aCGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGaa                                                                           <  1:1533095/140‑1 (MQ=255)                                                              aCGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGaa                                                                           <  1:1374256/140‑1 (MQ=255)                                                              aCGTAAAGACCATTAACCACAGATATGGCTGGCGCCGTCCGCGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGaa                                                                           >  2:1500607/1‑140 (MQ=255)                                                                  aaCGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACggg                                                                       >  2:1234735/1‑140 (MQ=255)                                                                   aCGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGt                                                                      <  2:743085/140‑1 (MQ=255)                                                                     gACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTaa                                                                    >  2:160789/1‑140 (MQ=255)                                                                         aTTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATAc                                                                <  1:567602/140‑1 (MQ=255)                                                                          ttGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAGAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACt                                                               >  2:1457096/1‑140 (MQ=255)                                                                          ttGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACt                                                               >  1:766637/1‑140 (MQ=255)                                                                          ttGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACt                                                               >  2:574565/1‑140 (MQ=255)                                                                          ttGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACt                                                               >  1:1527096/1‑140 (MQ=255)                                                                                    aaaCGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACg                                                     <  2:1428982/140‑1 (MQ=255)                                                                                        ggTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCg                                                 >  1:371548/1‑140 (MQ=255)                                                                                         gTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCgg                                                >  2:531833/1‑140 (MQ=255)                                                                                           tggtggCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCt                                              <  2:328141/140‑1 (MQ=255)                                                                                            ggtggCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCgg                                                >  1:709701/1‑137 (MQ=255)                                                                                            ggtggCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCgg                                                <  2:709701/137‑1 (MQ=255)                                                                                            ggtggCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGc                                               >  2:982883/1‑138 (MQ=255)                                                                                            ggtggCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGc                                               <  1:982883/138‑1 (MQ=255)                                                                                             gtggTTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGa                                            <  2:1532901/140‑1 (MQ=255)                                                                                              tggCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGAc                                           >  2:855599/1‑140 (MQ=255)                                                                                               ggCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTACTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGAcc                                          <  1:310848/140‑1 (MQ=255)                                                                                                gCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCa                                         <  2:371548/140‑1 (MQ=255)                                                                                                  ttACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCaaa                                       <  1:57613/140‑1 (MQ=255)                                                                                                  ttACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCaaa                                       >  1:790378/1‑140 (MQ=255)                                                                                                    aCGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCAAaca                                     >  1:635151/1‑140 (MQ=255)                                                                                                         tttGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCAAACACCAgc                                <  2:790378/140‑1 (MQ=255)                                                                                                          ttGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCAAACACCAgcg                               <  2:1258399/140‑1 (MQ=255)                                                                                                          ttGCTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCAAACACCAgcg                               <  1:531833/140‑1 (MQ=255)                                                                                                             cTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATTTTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCAAACACCAGCGCAc                            >  1:1350533/1‑140 (MQ=255)                                                                                                             cTCAAAGTAGTCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATTTTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCAAACACCAGCGCAc                            <  2:1350533/140‑1 (MQ=255)                                                                                                                  agtagtCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCAAACACCAGCGCACCAGTa                       >  2:1408533/1‑140 (MQ=255)                                                                                                                   gtagtCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCAAACACCAGCGCACCAGTaa                      <  1:1457096/140‑1 (MQ=255)                                                                                                                     agtCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCAAACACCAGCGCACCAGTAACg                    <  1:574565/140‑1 (MQ=255)                                                                                                                       tCGTAACCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCAAACACCAGCGCACCAGTAACGaa                  >  1:1472270/1‑140 (MQ=255)                                                                                                                           aaCCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCAAACACCAGCGCACCAGTAACGAACTgg              <  2:376880/140‑1 (MQ=255)                                                                                                                           aaCCCGGCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCAAACACCAGCGCACCAGTAACGAACTgg              <  1:855599/140‑1 (MQ=255)                                                                                                                                 gCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCAAACACCAGCGCACCAGTAACGAACTGGAat           >  1:1009805/1‑137 (MQ=255)                                                                                                                                 gCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCAAACACCAGCGCACCAGTAACGAACTGGAat           <  2:1009805/137‑1 (MQ=255)                                                                                                                                 gCTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCAAACACCAGCGCACCAGTAACGAACTGGAATAtc        >  2:579293/1‑140 (MQ=255)                                                                                                                                  cTTCAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCAAACACCAGCGCACCAGTAACGAACTGGAATAtct       <  2:1409385/140‑1 (MQ=255)                                                                                                                                     cAGTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCAAACACCAGCGCACCAGTAACGAACTGGAATATCtcat    <  1:1500607/140‑1 (MQ=255)                                                                                                                                       gTTACTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCAAACACCAGCGCACCAGTAACGAACTGGAATATCtcatca  <  1:579293/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTAAATACTCACTTGCACGCTCGGCTGACCAAACACCAGCGCACCAGTAACGAACTGGAATATCTCATCA  >  NC_000913/245080‑245353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑