Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,009,222	T→C	G153G (GGT→GGC)	ygeY →	putative peptidase YgeY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,009,222	0	T	C	98.9%	304.0 / ‑5.3	87	G153G (GGT→GGC)	ygeY	putative peptidase YgeY
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (44/42);  total (45/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGTCGCGGTACTTCCGACCAGGAAGGTGGCATGGCCTCTATGGTTTATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTCAGGGTGTT  >  NC_000913/3009087‑3009354                                                                                                                                        |                                                                                                                                     tggtCGCGGTACTTCCGACCAGGAAGGTGGCATGGCCTCTATGGTTTATGCCGGTAAAATCATTAAAGACCTTGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCtgt                                                                                                                                  <  1:287329/140‑1 (MQ=255) tggtCGCGGTACTTCCGACCAGGAAGGTGGCATGGCCTCTATGGTTTATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCtgt                                                                                                                                  >  1:1268271/1‑140 (MQ=255)      gcgGTACTTCCGACCAGGAAGGTGGCATGGCCTCTATGGTTTATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTgg                                                                                                                             <  2:643916/140‑1 (MQ=255)        ggTACTTCCGACCAGGAAGGTGGCATGGCCTCTATGGTTTATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCa                                                                                                                           <  2:1539385/140‑1 (MQ=255)        ggTACTTCCGACCAGGAAGGTGGCATGGCCTCTATGGTTTATGCCGGTAAAATCATCAAAGACCTCGGTCTGGAAGATGAATATTCCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGTCa                                                                                                                           <  1:957727/140‑1 (MQ=255)             ttCCGACCAGGAAGGTGGCATGGCCTCTATGGTTTATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACa                                                                                                                      <  1:1647074/140‑1 (MQ=255)              tCCGACCAGGAAGGTGGCATGGCCTCTATGGTTTATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTgg                                                                                                                             >  2:627354/1‑132 (MQ=255)              tCCGACCAGGAAGGTGGCATGGCCTCTATGGTTTATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTgg                                                                                                                             <  1:627354/132‑1 (MQ=255)               ccGACCAGGAAGGTGGCATGGCCTCTATGGTTTATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTa                                                                                                                        <  1:1095633/136‑1 (MQ=255)               ccGACCAGGAAGGTGGCATGGCCTCTATGGTTTATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTa                                                                                                                        >  2:1095633/1‑136 (MQ=255)                       gAAGGTGGCATGGCCTCTATGGTTTATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACa                                                                                                            >  1:751441/1‑140 (MQ=255)                               cATGGCCTCTATGGTTTATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCa                                                                                                    <  2:751441/140‑1 (MQ=255)                                aTGGCCTCTATGGTTTATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCAt                                                                                                   >  1:911985/1‑140 (MQ=255)                                  ggCCTCTATGGTTTATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTc                                                                                                 >  1:1523948/1‑140 (MQ=255)                                     ctctATGGTTTATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGcc                                                                                              <  2:1268271/140‑1 (MQ=255)                                        tATGGTTTATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCgg                                                                                           >  1:224294/1‑140 (MQ=255)                                             tttATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAAttt                                                                                      >  1:725173/1‑140 (MQ=255)                                             tttATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAAttt                                                                                      <  2:725173/140‑1 (MQ=255)                                             tttATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAAttt                                                                                      >  1:1150941/1‑140 (MQ=255)                                               tATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTtgt                                                                                    >  1:1137287/1‑140 (MQ=255)                                               tATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTtgt                                                                                    >  1:469331/1‑140 (MQ=255)                                               tATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTtgt                                                                                    >  2:151657/1‑140 (MQ=255)                                                 tGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTgg                                                                                  >  1:127982/1‑140 (MQ=255)                                                 tGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTgg                                                                                  >  1:843561/1‑140 (MQ=255)                                                  gCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGt                                                                                 >  1:1597988/1‑140 (MQ=255)                                                  gCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGt                                                                                 <  1:171079/140‑1 (MQ=255)                                                  gCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGt                                                                                 <  1:1309034/140‑1 (MQ=255)                                                  gCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGt                                                                                 >  2:1309034/1‑140 (MQ=255)                                                      gTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGt                                                                             >  2:28482/1‑140 (MQ=255)                                                          aaTCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCg                                                                         <  2:843561/140‑1 (MQ=255)                                                                tAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGaaccaa                                                                   >  1:1270784/1‑140 (MQ=255)                                                                tAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGaaccaa                                                                   >  2:1456928/1‑140 (MQ=255)                                                                  aaGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGaaccaacc                                                                 <  1:1456928/140‑1 (MQ=255)                                                                    gACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGa                                                               <  2:1597988/140‑1 (MQ=255)                                                                    gACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGa                                                               <  2:1523948/140‑1 (MQ=255)                                                                      ccTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACt                                                             <  1:28482/140‑1 (MQ=255)                                                                      ccTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACt                                                             <  1:1293264/140‑1 (MQ=255)                                                                        tCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCGGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGc                                                           <  1:829025/140‑1 (MQ=255)                                                                        tCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGc                                                           >  1:106372/1‑140 (MQ=255)                                                                        tCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGc                                                           <  2:911985/140‑1 (MQ=255)                                                                         cGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACt                                                             >  1:1298078/1‑137 (MQ=255)                                                                         cGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACt                                                             <  2:1298078/137‑1 (MQ=255)                                                                             cTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGt                                                      <  1:66276/140‑1 (MQ=255)                                                                                gAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGtata                                                   >  2:877900/1‑140 (MQ=255)                                                                                      gAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGa                                                               >  1:410595/1‑122 (MQ=255)                                                                                      gAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGa                                                               <  2:410595/122‑1 (MQ=255)                                                                                      gAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTgg                                             >  1:7863/1‑140 (MQ=255)                                                                                          ataCCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAg                                         >  2:1352759/1‑140 (MQ=255)                                                                                           taCCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAgc                                        >  1:1081680/1‑140 (MQ=255)                                                                                            aCCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAgcg                                       <  2:127982/140‑1 (MQ=255)                                                                                            aCCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAgcg                                       <  2:1081680/140‑1 (MQ=255)                                                                                            aCCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAgcg                                       >  1:771214/1‑140 (MQ=255)                                                                                              ccTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAgcg                                       >  2:591138/1‑138 (MQ=255)                                                                                              ccTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAgcg                                       <  1:591138/138‑1 (MQ=255)                                                                                              ccTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGcgcg                                     >  2:1571459/1‑140 (MQ=255)                                                                                                tgctgGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAgcg                                       >  2:789698/1‑136 (MQ=255)                                                                                                tgctgGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAgcg                                       <  1:789698/136‑1 (MQ=255)                                                                                                 gctgGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTc                                  >  2:1320265/1‑140 (MQ=255)                                                                                                 gctgGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTc                                  >  2:13315/1‑140 (MQ=255)                                                                                                 gctgGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTc                                  >  2:773086/1‑140 (MQ=255)                                                                                                  ctgGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCg                                 >  1:738806/1‑140 (MQ=255)                                                                                                  ctgGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCACTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCg                                 >  1:1116576/1‑140 (MQ=255)                                                                                                     gTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTAt                              >  2:1603355/1‑140 (MQ=255)                                                                                                        aCCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGa                           >  1:588103/1‑140 (MQ=255)                                                                                                        aCCGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGa                           <  2:469331/140‑1 (MQ=255)                                                                                                         ccGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGaa                          <  1:1320265/140‑1 (MQ=255)                                                                                                          cGGTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGaaa                         >  1:250894/1‑140 (MQ=255)                                                                                                           ggTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAAt                        <  2:7863/140‑1 (MQ=255)                                                                                                           ggTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAAt                        <  2:771214/140‑1 (MQ=255)                                                                                                            gTACGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAAtt                       >  2:949225/1‑140 (MQ=255)                                                                                                              aCGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCg                     <  2:745587/140‑1 (MQ=255)                                                                                                              aCGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCg                     <  2:106372/140‑1 (MQ=255)                                                                                                               cGGTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGt                    >  2:625740/1‑140 (MQ=255)                                                                                                                ggTGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCACTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTa                   <  2:1116576/140‑1 (MQ=255)                                                                                                                  tGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTAtt                 <  1:151657/140‑1 (MQ=255)                                                                                                                  tGCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTAtt                 <  2:588103/140‑1 (MQ=255)                                                                                                                   gCAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTg                <  2:1270784/140‑1 (MQ=255)                                                                                                                    cAGGAAGAAGACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGa               >  2:344332/1‑140 (MQ=255)                                                                                                                        aagaagACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGtt           <  1:1482567/140‑1 (MQ=255)                                                                                                                        aagaagACTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGtt           <  1:877900/140‑1 (MQ=255)                                                                                                                         agaagaCTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTc          <  2:763009/140‑1 (MQ=255)                                                                                                                         agaagaCTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTc          <  1:773086/140‑1 (MQ=255)                                                                                                                           aagaCTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTCAg        <  1:13835/140‑1 (MQ=255)                                                                                                                            agaCTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTCAgg       >  1:203688/1‑140 (MQ=255)                                                                                                                            agaCTGCGACGGCCCGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTCAgg       >  2:416931/1‑140 (MQ=255)                                                                                                                              aCTGCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTCAGGgt     >  1:1372351/1‑140 (MQ=255)                                                                                                                                 gCGACGGCCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTCAGGGTGtt  >  2:531523/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     TGGTCGCGGTACTTCCGACCAGGAAGGTGGCATGGCCTCTATGGTTTATGCCGGTAAAATCATTAAAGACCTCGGTCTGGAAGATGAATATACCCTGCTGGTTACCGGTACGGTGCAGGAAGAAGACTGCGACGGTCTGTGCTGGCAGTACATTATTGAACAATCCGGCATTCGCCCGGAATTTGTGGTCAGTACCGAACCAACCGACTGCCAGGTATACCGTGGTCAGCGCGGTCGTATGGAAATTCGTATTGATGTTCAGGGTGTT  >  NC_000913/3009087‑3009354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑