Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,083,348	G→A	A150A (GCC→GCT)	speB ←	agmatinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,083,348	0	G	A	100.0%	250.4 / NA	71	A150A (GCC→GCT)	speB	agmatinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/30);  total (41/30)

CCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGTGCGCCTG  >  NC_000913/3083214‑3083482                                                                                                                                       |                                                                                                                                       ccGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTc                                                                                                                                   <  1:162382/140‑1 (MQ=255)    ttGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCaaa                                                                                                                                >  1:1652015/1‑140 (MQ=255)    ttGTCTTTATCAAAATCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCaaa                                                                                                                                >  1:925898/1‑140 (MQ=255)      gTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGCGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAgt                                                                                                                              >  2:98337/1‑140 (MQ=255)          ttATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTAc                                                                                                                          <  2:1652015/140‑1 (MQ=255)                     ggTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCAttt                                                                                                               <  2:1632/140‑1 (MQ=255)                     ggTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCAttt                                                                                                               >  1:1215462/1‑140 (MQ=255)                       tACGAATACCAATCTGCACGGAATGGTTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTg                                                                                                             >  2:391367/1‑140 (MQ=255)                       tACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTg                                                                                                             <  2:325216/140‑1 (MQ=255)                       tACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTg                                                                                                             <  1:98337/140‑1 (MQ=255)                            aTACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGaa                                                                                                        >  2:735749/1‑140 (MQ=255)                               ccAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCTAAATg                                                                                                     <  1:481826/140‑1 (MQ=255)                               ccAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATg                                                                                                     <  1:735749/140‑1 (MQ=255)                               ccAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATg                                                                                                     >  2:287027/1‑140 (MQ=255)                                 aaTCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCt                                                                                                   >  2:729897/1‑140 (MQ=255)                                  aTCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCtt                                                                                                  <  2:594864/140‑1 (MQ=255)                                   tCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTc                                                                                                 >  2:372191/1‑140 (MQ=255)                                     tGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGc                                                                                               >  2:37037/1‑140 (MQ=255)                                       cACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCAt                                                                                             <  1:372191/140‑1 (MQ=255)                                           gAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCAt                                                                                             >  2:412713/1‑136 (MQ=255)                                           gAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCAt                                                                                             <  1:412713/136‑1 (MQ=255)                                            aaTGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCa                                                                                        >  1:1196338/1‑140 (MQ=255)                                                      gTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcg                                                                              >  1:168703/1‑140 (MQ=255)                                                      gTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcg                                                                              >  2:76829/1‑140 (MQ=255)                                                       tCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcgg                                                                             <  2:1411141/140‑1 (MQ=255)                                                         gATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggca                                                                           <  2:168703/140‑1 (MQ=255)                                                         gATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggca                                                                           <  2:1658790/140‑1 (MQ=255)                                                          aTCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcag                                                                          >  1:735414/1‑140 (MQ=255)                                                                ccTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcgg                                                                             >  1:1489697/1‑131 (MQ=255)                                                                ccTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGCTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcgg                                                                             <  2:1489697/131‑1 (MQ=255)                                                                    ccttCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAg                                                                >  1:1014697/3‑140 (MQ=255)                                                                         ggCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTc                                                           >  1:16938/1‑140 (MQ=255)                                                                         ggCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTc                                                           <  2:148315/140‑1 (MQ=255)                                                                           cgcgGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTcac                                                         <  2:1196338/140‑1 (MQ=255)                                                                              ggTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTcaccac                                                      <  1:729897/140‑1 (MQ=255)                                                                                     aaCATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAaga                                               <  2:1215462/140‑1 (MQ=255)                                                                                          aGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAGGTGGTgacca                                                       >  1:1577439/1‑127 (MQ=255)                                                                                          aGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAGGTGGTgacca                                                       <  2:1577439/127‑1 (MQ=255)                                                                                          aGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTc                                                           >  1:1059480/1‑123 (MQ=255)                                                                                          aGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTc                                                           <  2:1059480/123‑1 (MQ=255)                                                                                          aGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCa                                          >  1:672825/1‑140 (MQ=255)                                                                                          aGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCa                                          >  1:108744/1‑140 (MQ=255)                                                                                          aGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCa                                          <  2:735414/140‑1 (MQ=255)                                                                                          aGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCa                                          >  1:504127/1‑140 (MQ=255)                                                                                          aGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCa                                          >  2:1658778/1‑140 (MQ=255)                                                                                          aGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCa                                          >  2:1397806/1‑140 (MQ=255)                                                                                          aGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCa                                          >  2:1220005/1‑140 (MQ=255)                                                                                           gTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCAt                                         <  2:1014697/140‑1 (MQ=255)                                                                                            tGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATa                                        >  1:1481811/1‑140 (MQ=255)                                                                                            tGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATa                                        >  1:571034/1‑140 (MQ=255)                                                                                              ccGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGTGGCAGCGTAACAAAGTGGTCACCACCGaaa                                                 <  2:1222429/129‑1 (MQ=255)                                                                                              ccGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGTGGCAGCGTAACAAAGTGGTCACCACCGaaa                                                 >  1:1222429/1‑129 (MQ=255)                                                                                               cGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGc                                     >  2:470674/1‑140 (MQ=255)                                                                                                 tGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCtt                                   >  2:1363352/1‑140 (MQ=255)                                                                                                  ggTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTa                                  >  2:413166/1‑140 (MQ=255)                                                                                                   gTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTAc                                 >  2:217372/1‑140 (MQ=255)                                                                                                      aaaTTCACAACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCgg                              >  1:937483/1‑140 (MQ=255)                                                                                                             caACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGcagccag                       <  2:256862/140‑1 (MQ=255)                                                                                                             caACCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGcagccag                       <  2:186809/140‑1 (MQ=255)                                                                                                              aaCCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGcagccagc                      >  1:35570/1‑140 (MQ=255)                                                                                                               aCCGTTCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCcagca                     <  2:925898/140‑1 (MQ=255)                                                                                                                   ttCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCtt                 >  1:1572345/1‑140 (MQ=255)                                                                                                                    tCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTtc                >  2:156068/1‑140 (MQ=255)                                                                                                                    tCGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTtc                >  2:1210198/1‑140 (MQ=255)                                                                                                                     cGCATAGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCTCCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTtct               <  2:1481811/140‑1 (MQ=255)                                                                                                                          aGGTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCg          >  2:1412572/1‑140 (MQ=255)                                                                                                                           ggTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGt         <  1:217372/140‑1 (MQ=255)                                                                                                                            gTATCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGTg        <  2:504127/140‑1 (MQ=255)                                                                                                                               tCGGTGTGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGTgcgc     >  2:574567/1‑140 (MQ=255)                                                                                                                                 ggTGTGAGTGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGTGCGCCt   <  2:211934/140‑1 (MQ=255)                                                                                                                                  gtgtGAGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGTGCGCCTg  >  1:499891/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       CCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGTGCGCCTG  >  NC_000913/3083214‑3083482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑