Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,239,569	A→G	intergenic (+24/‑375)	alx → / → sstT	putative membrane‑bound redox modulator Alx/serine/threonine:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,239,569	0	A	G	100.0%	148.7 / NA	44	intergenic (+24/‑375)	alx/sstT	putative membrane‑bound redox modulator Alx/serine/threonine:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/21);  total (23/21)

TTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCG  >  NC_000913/3239432‑3239694                                                                                                                                          |                                                                                                                              ttCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGcc                                                                                                                             <  1:98904/140‑1 (MQ=255)  tCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGccc                                                                                                                            <  2:358623/140‑1 (MQ=255)  tCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGccc                                                                                                                            <  1:1084009/140‑1 (MQ=255)   cTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGT‑GATAATTTTTAATCTGCCTAAGCCGTGTGCCCTg                                                                                                                          <  1:1527185/140‑1 (MQ=255)           ttCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCa                                                                                                                       >  1:1212488/1‑136 (MQ=255)           ttCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCa                                                                                                                       <  2:1212488/136‑1 (MQ=255)           ttCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTaa                                                                                                                   >  1:504393/1‑140 (MQ=255)           ttCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTaa                                                                                                                   >  1:1499458/1‑140 (MQ=255)              cAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGccc                                                                                                                            <  1:985995/128‑1 (MQ=255)              cAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGccc                                                                                                                            >  2:985995/1‑128 (MQ=255)              cAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACAt                                                                                                                >  1:508605/1‑140 (MQ=255)                aTCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGa                                                                                                              >  2:1069789/1‑140 (MQ=255)                 tCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTAAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGcc                                                                                                                             >  2:1050569/1‑124 (MQ=255)                     cGTCTCGCTGGGCATGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCAcc                                                                                                         >  2:304605/1‑140 (MQ=255)                            cTGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTtct                                                                                                  <  2:504393/140‑1 (MQ=255)                             tGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGC‑GGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTtctcc                                                                                                <  2:536671/140‑1 (MQ=255)                                  gtggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTtctccctct                                                                                            <  1:1069789/140‑1 (MQ=255)                                  atggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTtctccctct                                                                                            <  1:304605/139‑1 (MQ=255)                                   tggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTtctccctctc                                                                                           >  1:15218/1‑140 (MQ=255)                                     gtgtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTtctccctctccc                                                                                         <  1:1428088/140‑1 (MQ=255)                                     gtgtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTtctccctctccc                                                                                         >  1:416575/1‑140 (MQ=255)                                         ttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTcc                                                                                     <  2:416575/140‑1 (MQ=255)                                              aTTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGga                                                                                <  1:1531479/140‑1 (MQ=255)                                                tCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTtctccctct                                                                                            >  2:632360/1‑126 (MQ=255)                                                tCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTtctccctct                                                                                            <  1:632360/126‑1 (MQ=255)                                                tCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGgaga                                                                              <  2:15218/140‑1 (MQ=255)                                                              ttattaTCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAgg                                                                >  1:200667/1‑140 (MQ=255)                                                                      aaCGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                        >  2:1655471/1‑140 (MQ=255)                                                                            ggggTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCCCCGTTTTCTCCCTCCCCCTCCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCAc                                                  <  2:508605/139‑1 (MQ=255)                                                                             gggTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCAcc                                                 >  1:1290889/1‑140 (MQ=255)                                                                              ggTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCg                                                >  2:1116453/1‑140 (MQ=255)                                                                                            aTGATAAGCAGCGGGGAGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTc                                  >  1:1042500/1‑140 (MQ=255)                                                                                                      gcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCACCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCt                        <  1:894300/140‑1 (MQ=255)                                                                                                        ggggTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTg                                     <  2:1532507/125‑1 (MQ=255)                                                                                                        ggggTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTg                                     >  1:1532507/1‑125 (MQ=255)                                                                                                         gggTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCt                              >  2:751949/1‑131 (MQ=255)                                                                                                         gggTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGGGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCt                              <  1:751949/131‑1 (MQ=255)                                                                                                          ggTGGATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAcc                    >  1:1014644/1‑140 (MQ=255)                                                                                                              gATAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCctcc                >  2:1418618/1‑139 (MQ=255)                                                                                                               aTAATTTTTAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCtctc               >  2:284651/1‑140 (MQ=255)                                                                                                                      ttAATCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAc        >  2:1630005/1‑140 (MQ=255)                                                                                                                        aaTCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgg      <  2:1499458/140‑1 (MQ=255)                                                                                                                        aaTCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgg      >  1:1347634/1‑140 (MQ=255)                                                                                                                        aaTCTGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgg      <  1:1393529/140‑1 (MQ=255)                                                                                                                            tGCCTAAGCCGTGTGCCCTGTCATTAACATGAGCCCCTTTTTCTCCCCCCCCCTCCCAGGGAGGGGGCCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCg  <  2:324023/140‑1 (MQ=25)                                                                                                                                          |                                                                                                                              TTCTACCATATTCCAATCGCCGTCTCGCTGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCG  >  NC_000913/3239432‑3239694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑