Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,601,156	T→C	T311A (ACG→GCG)	ftsX ←	cell division protein FtsX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,601,156	0	T	C	100.0%	122.3 / NA	35	T311A (ACG→GCG)	ftsX	cell division protein FtsX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/4);  total (31/4)

GCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAAT  >  NC_000913/3601021‑3601289                                                                                                                                        |                                                                                                                                       gCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCg                                                                                                                                    <  2:393584/140‑1 (MQ=255) gCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCg                                                                                                                                    >  1:241476/1‑140 (MQ=255) gCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCg                                                                                                                                    >  2:1455217/1‑140 (MQ=255)   ttttATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGaa                                                                                                                                  >  2:301075/1‑140 (MQ=255)          attCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCtgt                                                                                                                          <  1:886152/140‑1 (MQ=255)           ttCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCtgtg                                                                                                                         <  2:814709/140‑1 (MQ=255)             cAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGcc                                                                                                                       <  1:301075/140‑1 (MQ=255)             cAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGcc                                                                                                                       >  1:1662992/1‑140 (MQ=255)                     aaGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGc                                                                                                               >  2:224605/1‑140 (MQ=255)                          gCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAAccg                                                                                                          >  1:1346504/1‑140 (MQ=255)                           cGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAAccgc                                                                                                         >  1:356361/1‑140 (MQ=255)                                        cggggccAACCCACGCTCCCCCCCACCCAATCATCGAGCATACCAGCAGAAATAGCAGACATTCATCAAATAATAAGCCATTGATATAAAACTCCCCCCAAAAAC‑CCTGCCCCCCTTCCGCAACCGCCGATGACAATCgc                                                                                            <  2:1329378/134‑1 (MQ=255)                                           tGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCgcagc                                                                                         >  2:330253/1‑140 (MQ=255)                                             gCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCAc                                                                                       >  1:1329378/1‑140 (MQ=255)                                                                gccaaaTCACCGAGCAAACCAGAAAAAAGAGCAGGCATTCATAAAATAATAAGCTATTAATAACAAACTCCCCCCAAAAGC‑CCTGCCCCCCTCCCAAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAAtt                                                                    <  2:871381/136‑1 (MQ=255)                                                                            aGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAGA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGAcaacaac                                                        >  1:871381/1‑139 (MQ=255)                                                                               aTACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATgcg                                                     >  1:652911/1‑140 (MQ=255)                                                                                tACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATgcgc                                                    >  1:1048514/1‑140 (MQ=255)                                                                                tACCAGAAAAAAGAGCAGTCATTCAGCAAATAATAATCAATTAATAT‑AAACCTCCCCCCAAAAACCCTGCCCCCCCCCCGAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATgcgc                                                    <  2:465662/140‑1 (MQ=255)                                                                                   cagcagCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAg                                                 >  1:1330653/1‑140 (MQ=255)                                                                                    agcagcAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGa                                                >  1:535798/1‑140 (MQ=255)                                                                                       agcaATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGaaaa                                             >  1:844735/1‑140 (MQ=255)                                                                                           aTAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATccc                                         >  1:177597/1‑140 (MQ=255)                                                                                           aTAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATccc                                         >  1:1632403/1‑140 (MQ=255)                                                                                                aGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCAcagcag                                    >  1:1523582/1‑140 (MQ=255)                                                                                                 ggCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGt                                   >  1:718194/1‑140 (MQ=255)                                                                                                  gCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTTACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTg                                  >  2:1545229/1‑140 (MQ=255)                                                                                                       cATCGAATAATAAGCTATTAATATAAACCTCCGCCCCGAAAC‑CCTGTCCCCCTCCCACAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGcca                             <  1:696562/140‑1 (MQ=255)                                                                                                       cATCAAATGATAACCTTTTAATATCAAACTCCGCCCCAAAAC‑CCTGCCCCCCTCCCACAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGcca                             <  2:835267/140‑1 (MQ=255)                                                                                                        aTCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGccac                            >  1:835267/1‑140 (MQ=255)                                                                                                            aaTGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATa                        >  1:1065989/1‑140 (MQ=255)                                                                                                                aTAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAgg                    >  1:803300/1‑140 (MQ=255)                                                                                                                    gCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACg                >  1:805908/1‑140 (MQ=255)                                                                                                                    gCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACg                >  2:450296/1‑140 (MQ=255)                                                                                                                       aTTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAActgtc             >  1:1363696/1‑136 (MQ=255)                                                                                                                              tCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGAt      >  2:215105/1‑140 (MQ=255)                                                                                                                              tCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGAt      >  2:532800/1‑140 (MQ=255)                                                                                                                               cAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATg     >  2:1435717/1‑140 (MQ=255)                                                                                                                               cAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATg     >  1:1186221/1‑140 (MQ=255)                                                                                                                                  aCTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGaac  >  1:1360318/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                       GCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAAT  >  NC_000913/3601021‑3601289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑