Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,602,485	T→C	H88R (CAC→CGC)	ftsE ←	cell division protein FtsE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,602,485	0	T	C	100.0%	308.4 / NA	86	H88R (CAC→CGC)	ftsE	cell division protein FtsE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (51/35);  total (51/35)

GTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGG  >  NC_000913/3602349‑3602619                                                                                                                                         |                                                                                                                                       gTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGAt                                                                                                                                     <  2:128172/140‑1 (MQ=255)  ttcttcGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATc                                                                                                                                    <  1:1031970/140‑1 (MQ=255)  ttcttcGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATc                                                                                                                                    >  2:1031970/1‑140 (MQ=255)  ttcttcGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATc                                                                                                                                    >  2:661674/1‑140 (MQ=255)      tcGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTgg                                                                                                                                <  1:671835/140‑1 (MQ=255)       cGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGa                                                                                                                               >  1:439741/1‑140 (MQ=255)       cGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGa                                                                                                                               >  1:648529/1‑140 (MQ=255)       cGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGa                                                                                                                               >  1:660093/1‑140 (MQ=255)                   tAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCaa                                                                                                                   >  2:875402/1‑140 (MQ=255)                      cccGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCTGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCt                                                                                                                >  2:57223/1‑140 (MQ=255)                      cccGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCt                                                                                                                >  1:337006/1‑140 (MQ=255)                        cGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTgg                                                                                                              >  2:238667/1‑140 (MQ=255)                        cGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTgg                                                                                                              >  1:1650231/1‑140 (MQ=255)                        cGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTgg                                                                                                              <  1:875402/140‑1 (MQ=255)                          aCTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTggcg                                                                                                            >  1:1646519/1‑140 (MQ=255)                           cTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTggcgg                                                                                                           >  1:1262028/1‑140 (MQ=255)                            tttATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTggcggc                                                                                                          >  1:1542422/1‑140 (MQ=255)                                     cgccgcCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACg                                                                                                 >  2:356373/1‑140 (MQ=255)                                                           atatCGTCACCGCTGGCACCGGCGATGATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGAcg                                                                           >  1:648088/1‑140 (MQ=255)                                                                 tCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGAt                                                                     >  1:1309574/1‑140 (MQ=255)                                                                  cACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATg                                                                    >  1:81164/1‑140 (MQ=255)                                                                   aCCGCTGGCACCGGCGATGATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGt                                                                   <  2:648088/140‑1 (MQ=255)                                                                   aCCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGt                                                                   <  2:439741/140‑1 (MQ=255)                                                                      gCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCAt                                                                <  1:455919/140‑1 (MQ=255)                                                                       cTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAGACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATg                                                               >  2:1188955/1‑140 (MQ=255)                                                                       cTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATg                                                               <  2:648529/140‑1 (MQ=255)                                                                        tGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATgg                                                              >  1:94294/1‑140 (MQ=255)                                                                           cACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCg                                                           <  2:1646519/140‑1 (MQ=255)                                                                                 cGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAAc                                                     >  1:1059746/1‑140 (MQ=255)                                                                                    tAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGG‑AAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGa                                                 >  1:804445/1‑140 (MQ=255)                                                                                     aaTCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGa                                                 >  1:576542/1‑140 (MQ=255)                                                                                           cGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCg                                                           >  1:184918/1‑124 (MQ=255)                                                                                           cGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCg                                                           <  2:184918/124‑1 (MQ=255)                                                                                           cGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTcc                                           >  1:1040028/1‑140 (MQ=255)                                                                                           cGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTcc                                           >  1:366731/1‑140 (MQ=255)                                                                                            gggATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAGACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTccc                                          <  1:1188955/140‑1 (MQ=255)                                                                                                    cACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTgg                                  <  2:1501561/140‑1 (MQ=255)                                                                                                     aCGTTATCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTggg                                 <  2:81164/140‑1 (MQ=255)                                                                                                          aTCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCt                            <  2:1309574/140‑1 (MQ=255)                                                                                                          aTCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCt                            <  1:633229/140‑1 (MQ=255)                                                                                                           tCGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTc                           <  2:576542/140‑1 (MQ=255)                                                                                                            cGTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCa                          <  1:92330/140‑1 (MQ=255)                                                                                                             gTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCaa                         <  1:238667/140‑1 (MQ=255)                                                                                                             gTAGACAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCaa                         <  1:1001669/140‑1 (MQ=255)                                                                                                                 agaGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATccc                     >  2:46164/3‑140 (MQ=255)                                                                                                                 aCAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATccc                     >  2:1598086/1‑140 (MQ=255)                                                                                                                 aCAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATACCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATccc                     >  2:930653/1‑140 (MQ=255)                                                                                                                  cAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCca                    <  1:661674/140‑1 (MQ=255)                                                                                                                  cAGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCca                    <  2:1650231/140‑1 (MQ=255)                                                                                                                   aGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCt                            >  2:419721/1‑131 (MQ=255)                                                                                                                   aGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCt                            <  1:419721/131‑1 (MQ=255)                                                                                                                   aGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCcac                   >  2:216818/1‑140 (MQ=255)                                                                                                                   aGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCcac                   <  2:94294/140‑1 (MQ=255)                                                                                                                   aGTACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCcac                   >  2:736872/1‑140 (MQ=255)                                                                                                                     tACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCaa                         >  2:347697/1‑132 (MQ=255)                                                                                                                     tACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCaa                         <  1:347697/132‑1 (MQ=255)                                                                                                                     tACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAg                 >  2:280646/1‑140 (MQ=255)                                                                                                                     tACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAg                 >  2:95820/1‑140 (MQ=255)                                                                                                                     tACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAg                 >  1:1035479/1‑140 (MQ=255)                                                                                                                     tACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAg                 >  1:1659242/1‑140 (MQ=255)                                                                                                                     tACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAg                 >  1:19654/1‑140 (MQ=255)                                                                                                                     tACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAg                 >  2:1362479/1‑140 (MQ=255)                                                                                                                     tACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAg                 >  1:464115/1‑140 (MQ=255)                                                                                                                     tACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAg                 >  1:519604/1‑140 (MQ=255)                                                                                                                     tACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAg                 >  2:1025705/1‑140 (MQ=255)                                                                                                                     tACGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTCTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAg                 >  2:714856/1‑140 (MQ=255)                                                                                                                      aCGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGa                <  2:519604/140‑1 (MQ=255)                                                                                                                       cGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGAt               <  1:1175423/140‑1 (MQ=255)                                                                                                                       cGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGAt               <  1:523380/140‑1 (MQ=255)                                                                                                                       cGGTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGAt               <  1:1362479/140‑1 (MQ=255)                                                                                                                        ggTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATc              <  2:464115/140‑1 (MQ=255)                                                                                                                        ggTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATc              <  2:1542422/140‑1 (MQ=255)                                                                                                                         gTCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCa             <  2:19654/140‑1 (MQ=255)                                                                                                                          tCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAg            >  1:621699/1‑140 (MQ=255)                                                                                                                          tCCATCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTACCGGCGCTGGGCCGCTCAACCCCACAGATCAg            >  2:1469052/1‑140 (MQ=255)                                                                                                                             aTCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCtt         >  1:1634543/1‑140 (MQ=255)                                                                                                                             aTCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCtt         >  2:1457614/1‑140 (MQ=255)                                                                                                                              tCAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTc        <  2:660093/140‑1 (MQ=255)                                                                                                                               cAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCcac                   >  1:247796/1‑128 (MQ=255)                                                                                                                               cAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCcac                   <  2:247796/128‑1 (MQ=255)                                                                                                                               cAGTAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCa       >  1:90140/1‑140 (MQ=255)                                                                                                                                  tAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCagga    <  2:1262028/140‑1 (MQ=255)                                                                                                                                  tAGATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCagga    <  1:95820/140‑1 (MQ=255)                                                                                                                                    gATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCaggagg  >  2:732156/1‑140 (MQ=255)                                                                                                                                    gATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCaggagg  >  2:1241564/1‑140 (MQ=255)                                                                                                                                    gATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCaggagg  >  1:512584/1‑140 (MQ=255)                                                                                                                                    gATGGCGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCaggagg  >  2:306998/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       GTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGG  >  NC_000913/3602349‑3602619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑