Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,083,781	T→C	N681S (AAC→AGC)	fdoG ←	formate dehydrogenase O subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,083,781	0	T	C	100.0%	347.5 / NA	97	N681S (AAC→AGC)	fdoG	formate dehydrogenase O subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (54/43);  total (54/43)

AACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGCGTCCGCACCTTTCCAGTG  >  NC_000913/4083652‑4083917                                                                                                                                  |                                                                                                                                         aacgaacTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCcatgtcat                                                                                                                                >  1:294821/1‑140 (MQ=255)       cTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAg                                                                                                                          >  1:1309490/1‑140 (MQ=255)            ttgttgGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCt                                                                                                                     >  1:673175/1‑140 (MQ=255)               ttgGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGt                                                                                                                  >  2:903535/1‑140 (MQ=255)               ttgGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGt                                                                                                                  >  1:1430454/1‑140 (MQ=255)               ttgGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGt                                                                                                                  >  1:600901/1‑140 (MQ=255)               ttgGCCTTTCTTGACGATAACCGCACCGGTCGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGt                                                                                                                  <  2:1163981/140‑1 (MQ=255)                 ggCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTcc                                                                                                                >  1:296950/1‑140 (MQ=255)                 ggCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTcc                                                                                                                <  1:575780/140‑1 (MQ=255)                      ttCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGtt                                                                                                           >  1:415052/1‑140 (MQ=255)                         ttGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTcgc                                                                                                        >  2:233589/1‑140 (MQ=255)                            aCGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGcc                                                                                                     >  2:270356/1‑140 (MQ=255)                               aTAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCAcc                                                                                                  <  2:1430454/140‑1 (MQ=255)                                tAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCAccc                                                                                                 >  2:980849/1‑140 (MQ=255)                                 aaCCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCt                                                                                                >  2:135684/1‑140 (MQ=255)                                 aaCCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCt                                                                                                >  2:777154/1‑140 (MQ=255)                                 aaCCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCATTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCt                                                                                                >  2:727469/1‑140 (MQ=255)                                   ccGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGt                                                                                              <  2:636285/140‑1 (MQ=255)                                   ccGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCACCACCCTGt                                                                                              <  2:887088/140‑1 (MQ=255)                                     gCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTc                                                                                            <  2:600901/140‑1 (MQ=255)                                     gCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTc                                                                                            <  2:673175/140‑1 (MQ=255)                                        ccGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGc                                                                                         >  1:675221/1‑140 (MQ=255)                                          ggTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCAt                                                                                       >  2:553504/1‑140 (MQ=255)                                          ggTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCAt                                                                                       <  2:294821/140‑1 (MQ=255)                                           gTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCa                                                                                                    <  1:677897/126‑1 (MQ=255)                                           gTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCa                                                                                                    >  2:677897/1‑126 (MQ=255)                                           gTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATa                                                                                      >  2:1329633/1‑140 (MQ=255)                                            ttGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATAc                                                                                     >  2:1575897/1‑140 (MQ=255)                                             tGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACa                                                                                    >  1:282300/1‑140 (MQ=255)                                             tGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACa                                                                                    <  2:282300/140‑1 (MQ=255)                                             tGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACa                                                                                    <  1:233589/140‑1 (MQ=255)                                                cGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCt                                                                                 >  2:1358312/1‑140 (MQ=255)                                                    tCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTgcg                                                                             <  1:1393526/140‑1 (MQ=255)                                                     cGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTgcgc                                                                            <  2:1309490/140‑1 (MQ=255)                                                     cGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTgcgc                                                                            <  1:270356/140‑1 (MQ=255)                                                                gCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGaa                                                                 >  2:1574809/1‑140 (MQ=255)                                                                gCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGaa                                                                 >  1:398403/1‑140 (MQ=255)                                                                gCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGaa                                                                 >  1:804718/1‑140 (MQ=255)                                                                 ccAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAg                                                                >  2:243122/1‑140 (MQ=255)                                                                 ccAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAg                                                                >  1:713451/1‑140 (MQ=255)                                                                  cAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTgcg                                                                             <  2:1053065/126‑1 (MQ=255)                                                                  cAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTgcg                                                                             >  1:1053065/1‑126 (MQ=255)                                                                  cAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGa                                                               >  1:129012/1‑140 (MQ=255)                                                                    gcgcCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATa                                                             <  1:1329633/140‑1 (MQ=255)                                                                     cgcCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATAc                                                            <  2:713451/140‑1 (MQ=255)                                                                     cgcCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATAc                                                            <  2:675221/140‑1 (MQ=255)                                                                     cgcCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATAc                                                            >  2:575950/1‑140 (MQ=255)                                                                          tACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGgag                                                       >  2:939906/1‑140 (MQ=255)                                                                             cGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAgg                                                    <  1:1358312/140‑1 (MQ=255)                                                                               ttGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATa                                                             <  1:936111/129‑1 (MQ=255)                                                                               ttGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATa                                                             >  2:936111/1‑129 (MQ=255)                                                                               ttGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGAt                                                  <  1:821621/140‑1 (MQ=255)                                                                               ttGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGAt                                                  <  2:804718/140‑1 (MQ=255)                                                                               ttGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGAt                                                  >  1:776257/1‑140 (MQ=255)                                                                                 gCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGAtct                                                <  1:777154/140‑1 (MQ=255)                                                                                  ctctCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGAtctc                                               >  2:1227672/1‑140 (MQ=255)                                                                                    ctcCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCg                                              >  2:1610874/1‑139 (MQ=255)                                                                                    ctcCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCg                                              <  1:1610874/139‑1 (MQ=255)                                                                                     tcCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGcc                                            >  2:1436990/1‑140 (MQ=255)                                                                                      ccATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCg                                           <  2:381710/140‑1 (MQ=255)                                                                                       cATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCgt                                          >  2:988584/1‑140 (MQ=255)                                                                                       cATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCgt                                          >  1:539444/1‑140 (MQ=255)                                                                                          cGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCgtcag                                       >  2:560040/1‑140 (MQ=255)                                                                                            ccACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCgtcagtc                                     >  1:1053161/1‑140 (MQ=255)                                                                                            ccACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCgtcagtc                                     >  1:649141/1‑140 (MQ=255)                                                                                                 tcttctGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAgcgc                                <  2:1283049/140‑1 (MQ=255)                                                                                                  cttctGAAGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCa                               <  1:313215/140‑1 (MQ=255)                                                                                                        aaGATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATcccc                         <  1:1575897/140‑1 (MQ=255)                                                                                                          gATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAgcgc                                >  2:752969/1‑131 (MQ=255)                                                                                                          gATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAgcgc                                <  1:752969/131‑1 (MQ=255)                                                                                                          gATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCaa                              <  1:1079579/133‑1 (MQ=255)                                                                                                          gATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCaa                              >  2:1079579/1‑133 (MQ=255)                                                                                                          gATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCgg                       >  1:1214480/1‑140 (MQ=255)                                                                                                          gATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCgg                       >  2:298470/1‑140 (MQ=255)                                                                                                          gATGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCgg                       >  1:644734/1‑140 (MQ=255)                                                                                                           aTGGCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCggg                      >  2:549323/1‑140 (MQ=255)                                                                                                             ggCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCggg                      <  2:1618192/138‑1 (MQ=255)                                                                                                             ggCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCggg                      >  1:1618192/1‑138 (MQ=255)                                                                                                             ggCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGc                    >  1:43297/1‑140 (MQ=255)                                                                                                             ggCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGc                    >  2:899329/1‑140 (MQ=255)                                                                                                              gCTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGCg                   >  1:1278306/1‑140 (MQ=255)                                                                                                               cTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGCGt                  >  2:329950/1‑140 (MQ=255)                                                                                                               cTCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGCGt                  >  2:1066182/1‑140 (MQ=255)                                                                                                                tCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCCCCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGCGTc                 <  2:649141/140‑1 (MQ=255)                                                                                                                tCATGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGCGTc                 <  2:1053161/140‑1 (MQ=255)                                                                                                                  aTGCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGCGTCCg               >  1:782841/1‑140 (MQ=255)                                                                                                                    gCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGCGTCCGCa             <  1:243122/140‑1 (MQ=255)                                                                                                                    gCGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGCGTCCGCa             >  2:906625/1‑140 (MQ=255)                                                                                                                     cGGAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGCGTCCGCAc            <  1:575950/140‑1 (MQ=255)                                                                                                                      ggAATGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGCGTCCGCAcc           <  1:1436990/140‑1 (MQ=255)                                                                                                                        aaTGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGCGTCCGCACCtt         <  2:43297/140‑1 (MQ=255)                                                                                                                          tGGCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGCGTCCGCACCTTTc       <  2:415052/140‑1 (MQ=255)                                                                                                                           ggCGTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGCGTCCGCACCTTTcc      <  1:906625/140‑1 (MQ=255)                                                                                                                              gTAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGCGTCCGCACCTTTCCAGt   <  1:131335/140‑1 (MQ=255)                                                                                                                               tAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGCGTCCGCACCTTTCCAGTg  <  1:727469/140‑1 (MQ=255)                                                                                                                               tAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGCGTCCGCACCTTTCCAGTg  <  1:554045/140‑1 (MQ=255)                                                                                                                               tAGCTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGCGTCCGCACCTTTCCAGTg  <  2:539444/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                         AACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCATACATCTTGCGCAAGCGCAGGAAGATACCGGAGAGGATCTCGCCGTCAGTCAGCGCAATCCCCGGGGCGTCCGCACCTTTCCAGTG  >  NC_000913/4083652‑4083917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑