Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,122,748	A→G	S198P (TCT→CCT)	ftsN ←	cell division protein FtsN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,122,748	0	A	G	100.0%	159.0 / NA	45	S198P (TCT→CCT)	ftsN	cell division protein FtsN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/21);  total (24/21)

TTCTCCGCCGTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTA  >  NC_000913/4122615‑4122882                                                                                                                                      |                                                                                                                                       ttCTCCGCCGTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGgcg                                                                                                                                  <  1:1126428/140‑1 (MQ=255)     ccgccgTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGt                                                                                                                              <  1:7694/140‑1 (MQ=255)     ccgccgTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGt                                                                                                                              <  1:1224877/140‑1 (MQ=255)        ccgTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTgc                                                                                                                           >  1:339139/1‑140 (MQ=255)          gTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTgcgc                                                                                                                         <  2:851270/140‑1 (MQ=255)                     cgcgTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTg                                                                                                              <  1:292565/140‑1 (MQ=255)                         tCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGc                                                                                                          >  1:68301/1‑140 (MQ=255)                         tCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGc                                                                                                          >  2:1003903/1‑140 (MQ=255)                                  cGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCg                                                                                                     >  1:1645114/1‑136 (MQ=255)                                  cGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCg                                                                                                     <  2:1645114/136‑1 (MQ=255)                                       aaCTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTAcgcg                                                                                            <  1:1003903/140‑1 (MQ=255)                                          tGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTct                                                                                         >  2:269449/1‑140 (MQ=255)                                            gcgcAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTctgc                                                                                       <  2:339139/140‑1 (MQ=255)                                               cAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTctgctgc                                                                                    >  1:614779/1‑140 (MQ=255)                                                       tgcGGCTTCGACTGTGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCt                                                                            <  1:538891/140‑1 (MQ=255)                                                            cTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGtt                                                                       >  2:634826/1‑140 (MQ=255)                                                               cGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAg                                                                    <  1:1021156/140‑1 (MQ=255)                                                                       cAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTAcgc                                                            <  2:1563782/140‑1 (MQ=255)                                                                        aGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTAcgcg                                                           <  1:634826/140‑1 (MQ=255)                                                                               tgCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGActgctg                                                    >  1:634354/1‑140 (MQ=255)                                                                                  gcAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGc                                                 <  2:68301/140‑1 (MQ=255)                                                                                   cAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGcc                                                <  2:614779/140‑1 (MQ=255)                                                                                    aGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGcc                                                <  2:1470465/139‑1 (MQ=255)                                                                                    aGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGcc                                                >  1:1470465/1‑139 (MQ=255)                                                                                    aGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGAGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCa                                               >  2:1320647/1‑140 (MQ=255)                                                                                      gAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAgg                                             <  1:1320647/140‑1 (MQ=255)                                                                                           tGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTg                                        <  1:269449/140‑1 (MQ=255)                                                                                                cagATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGtt                                   <  2:1010644/140‑1 (MQ=255)                                                                                                  gATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTcc                                 >  2:1209284/1‑140 (MQ=255)                                                                                                            cGGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATtgct                       >  2:797919/1‑140 (MQ=255)                                                                                                             ggCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATtgctg                      >  1:1562015/1‑140 (MQ=255)                                                                                                             ggCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATtgctg                      >  1:1325947/1‑140 (MQ=255)                                                                                                              gctgctGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTGCGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATtgctgc                     >  1:607842/1‑140 (MQ=255)                                                                                                               ctgctgAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATtgc                        <  1:1324238/136‑1 (MQ=255)                                                                                                               ctgctgAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTgcg                    >  1:992874/1‑140 (MQ=255)                                                                                                               ctgctgAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTgcg                    >  1:511628/1‑140 (MQ=255)                                                                                                               ctgctgAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCGGAGCCAGGCGTTGCTGTTCCGCTAATtgc                        >  2:1324238/1‑136 (MQ=255)                                                                                                               ctgctgAGAAGAAGCCGGTTTAGGCTGGCGCGGCTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACCGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTgcg                    >  2:1624416/1‑140 (MQ=255)                                                                                                                       aagaagCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGt            <  2:992874/140‑1 (MQ=255)                                                                                                                       aagaagCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGt            >  2:1025445/1‑140 (MQ=255)                                                                                                                       aagaagCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCACTAATTGCTGCGCCTGACGt            <  2:246796/140‑1 (MQ=255)                                                                                                                          aagCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTgg         >  1:901079/1‑140 (MQ=255)                                                                                                                            gCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGgcg       >  2:1132314/1‑140 (MQ=255)                                                                                                                             ccGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGgcgc      >  1:1493839/1‑140 (MQ=255)                                                                                                                                 tttAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTa  >  2:771611/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       TTCTCCGCCGTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTA  >  NC_000913/4122615‑4122882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑