Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,295,358	C→T	A293V (GCC→GTC)	gltP →	glutamate/aspartate : H(+) symporter GltP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,295,358	0	C	T	99.0%	335.4 / NA	96	A293V (GCC→GTC)	gltP	glutamate/aspartate : H(+) symporter GltP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (45/50);  minor base G (0/1);  total (45/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATCGCGCAGTTGTATGGCATTGACCTGTCCATCT  >  NC_000913/4295225‑4295492                                                                                                                                      |                                                                                                                                       cTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACgg                                                                                                                                  >  1:581978/1‑140 (MQ=255) cTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACgg                                                                                                                                  >  1:1462256/1‑140 (MQ=255)    ggAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGc                                                                                                                               <  2:1462256/140‑1 (MQ=255)     gAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGcc                                                                                                                              >  2:134181/1‑139 (MQ=255)     gAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCa                                                                                                                              >  2:326303/1‑140 (MQ=255)      aaTTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACgg                                                                                                                                  >  1:435811/1‑135 (MQ=255)      aaTTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACgg                                                                                                                                  <  2:435811/135‑1 (MQ=255)      aaTTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCAc                                                                                                                             >  2:983895/1‑140 (MQ=255)        ttGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGGTGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCg                                                                                                                           <  1:1593116/140‑1 (MQ=255)         tgtgGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCgg                                                                                                                          >  2:1497321/1‑140 (MQ=255)           tgGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGgtg                                                                                                                        >  1:1498251/1‑140 (MQ=255)              cgcgcCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCg                                                                                                                     <  2:93520/140‑1 (MQ=255)              cgcgcCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCg                                                                                                                     >  2:782112/1‑140 (MQ=255)                cgcCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGAt                                                                                                                   >  2:471242/1‑140 (MQ=255)                   cTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCAc                                                                                                                >  2:452123/1‑140 (MQ=255)                   cTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCAc                                                                                                                <  2:581978/140‑1 (MQ=255)                             tAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGGCTACGGAGCACCGGTGTCGATCACCAGTTTCgtg                                                                                                      <  2:1600144/140‑1 (MQ=255)                               aGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCgtggt                                                                                                    <  1:1111451/140‑1 (MQ=255)                                gCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCgtggtg                                                                                                   >  1:1138291/1‑140 (MQ=255)                                   tCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCg                                                                                                <  1:51433/140‑1 (MQ=255)                                   tCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCg                                                                                                <  2:212316/140‑1 (MQ=255)                                     tGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGAc                                                                                              >  1:60275/1‑140 (MQ=255)                                      ggATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGAcc                                                                                             <  2:1138291/140‑1 (MQ=255)                                           cTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTa                                                                                        <  2:949962/140‑1 (MQ=255)                                           cTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTa                                                                                        <  1:326303/140‑1 (MQ=255)                                           cTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTa                                                                                        <  1:983895/140‑1 (MQ=255)                                           cTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTa                                                                                        <  1:513396/140‑1 (MQ=255)                                           cTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTa                                                                                        <  1:1497321/140‑1 (MQ=255)                                             gATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTa                                                                                        <  1:786667/138‑1 (MQ=255)                                             gATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTa                                                                                        >  2:786667/1‑138 (MQ=255)                                                   tATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTa                                                                                >  1:1452679/1‑140 (MQ=255)                                                     ttCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAAc                                                                              <  1:452123/140‑1 (MQ=255)                                                               tGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGtt                                                                    <  1:782112/140‑1 (MQ=255)                                                                 aGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCg                                                                  >  1:803553/1‑140 (MQ=255)                                                                 aGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCg                                                                  >  2:283772/1‑140 (MQ=255)                                                                 aGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCg                                                                  >  1:78086/1‑140 (MQ=255)                                                                  gCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGa                                                                 <  2:594174/140‑1 (MQ=255)                                                                  gCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGa                                                                 <  2:580144/140‑1 (MQ=255)                                                                    tGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACg                                                               <  1:986654/140‑1 (MQ=255)                                                                    tGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACg                                                               <  1:790439/140‑1 (MQ=255)                                                                    tGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACg                                                               <  1:283772/140‑1 (MQ=255)                                                                       ttCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTg                                                            <  2:60275/140‑1 (MQ=255)                                                                           ggCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATc                                                        >  1:1282631/1‑140 (MQ=255)                                                                            gCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCa                                                       >  1:257506/1‑140 (MQ=255)                                                                             cGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACg                                                               <  1:522779/131‑1 (MQ=255)                                                                             cGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACg                                                               >  2:522779/1‑131 (MQ=255)                                                                                aCTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGt                                                   >  1:737691/1‑140 (MQ=255)                                                                                 cTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTTGACGCTGTATCAAAGTa                                                  >  1:530719/1‑140 (MQ=255)                                                                                 cTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGc                                                              <  1:429307/128‑1 (MQ=255)                                                                                 cTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGc                                                              >  2:429307/1‑128 (MQ=255)                                                                                  tCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTAt                                                 >  2:214429/1‑140 (MQ=255)                                                                                  tCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTAt                                                 <  2:737691/140‑1 (MQ=255)                                                                                      cTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGcc                                             <  1:214429/140‑1 (MQ=255)                                                                                      cTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGcc                                             <  1:992852/140‑1 (MQ=255)                                                                                      cTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGcc                                             >  2:854675/1‑140 (MQ=255)                                                                                      cTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGcc                                             >  2:1083098/1‑140 (MQ=255)                                                                                      cTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGcc                                             >  2:1261401/1‑140 (MQ=255)                                                                                       tGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCg                                            <  1:1083098/140‑1 (MQ=255)                                                                                         ccAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCt                                          >  2:1119100/1‑140 (MQ=255)                                                                                         ccAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCt                                          >  1:69526/1‑140 (MQ=255)                                                                                           aGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTAt                                        <  1:1261401/140‑1 (MQ=255)                                                                                            gCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATc                                       >  1:546867/1‑140 (MQ=255)                                                                                            gCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATc                                       >  1:157828/1‑140 (MQ=255)                                                                                                tGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCa                                   <  2:803553/140‑1 (MQ=255)                                                                                                tGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCa                                   <  1:334136/140‑1 (MQ=255)                                                                                                tGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCa                                   >  1:1011538/1‑140 (MQ=255)                                                                                                 gAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCAt                                  >  1:109053/1‑140 (MQ=255)                                                                                                  aaaGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTTATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATc                                 >  1:228065/1‑140 (MQ=255)                                                                                                   aaGCGTGCTGTCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATcg                                >  1:462892/1‑140 (MQ=255)                                                                                                   aaGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATcg                                <  2:530719/140‑1 (MQ=255)                                                                                                   aaGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATcg                                <  2:1498251/140‑1 (MQ=255)                                                                                                     gCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATcgcg                              <  2:1452679/140‑1 (MQ=255)                                                                                                     gCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATcgcg                              <  2:546867/140‑1 (MQ=255)                                                                                                     gCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATcgcg                              <  1:1358943/140‑1 (MQ=255)                                                                                                     gCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATcgcg                              <  2:69526/140‑1 (MQ=255)                                                                                                     gCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATcgcg                              <  1:173287/140‑1 (MQ=255)                                                                                                     gCGTGCTGCCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATcgcg                              <  2:1011538/140‑1 (MQ=255)                                                                                                        tgctgcCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATcgcg                              <  1:1516597/137‑1 (MQ=255)                                                                                                        tgctgcCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATcgcg                              >  2:670036/1‑137 (MQ=255)                                                                                                        tgctgcCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATcgcg                              >  2:1516597/1‑137 (MQ=255)                                                                                                        tgctgcCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATcgcg                              <  1:670036/137‑1 (MQ=255)                                                                                                          ctgcCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTAt                                        >  1:1092304/1‑125 (MQ=255)                                                                                                          ctgcCGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTAt                                        <  2:1092304/125‑1 (MQ=255)                                                                                                             ccGCGAATTCTTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATCGCGCAGTTGTa                      <  2:1591024/140‑1 (MQ=255)                                                                                                             ccGCGAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATCGCGCAGTTGTa                      <  2:109053/140‑1 (MQ=255)                                                                                                                cgAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATcgc                               >  2:1071574/1‑128 (MQ=255)                                                                                                                cgAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATcgc                               <  1:1071574/128‑1 (MQ=255)                                                                                                                cgAATTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATCGCGCAGTTGTATgg                   >  1:326300/1‑140 (MQ=255)                                                                                                                  aaTTATTGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATCGCGCAGTTGTATGGCa                 <  2:1282631/140‑1 (MQ=255)                                                                                                                    ttattGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATCGCACAGTTGTATGGCAtt               <  1:1290896/140‑1 (MQ=255)                                                                                                                     tattGAGAAGATGGAAGTCTACGGAGC‑CCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATCGCGCAGTTGTATGGCATTGa             <  1:134181/140‑1 (MQ=255)                                                                                                                        tGAGAAGATGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATCGCGCAGTTGTATGGCATTGAcc           <  1:491926/140‑1 (MQ=255)                                                                                                                            aagaTGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATCGCGCAGTTGTATGGCATTGACCTGTc       >  1:1173993/1‑140 (MQ=255)                                                                                                                            aagaTGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATCGCGCAGTTGTATGGCATTGACCTGTc       >  1:355004/1‑140 (MQ=255)                                                                                                                               aTGGAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATCGCGCAGTTGTATGGCATTGACCTGTCCAt    >  2:227059/1‑140 (MQ=255)                                                                                                                                 ggAAGTCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATCGCGCAGTTGTATGGCATTGACCTGTCCATCt  >  1:1421896/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       CTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCTGTATCAAAGTATTGCCGCTATCTTCATCGCGCAGTTGTATGGCATTGACCTGTCCATCT  >  NC_000913/4295225‑4295492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑