Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,455,281	T→C	D110G (GAC→GGC)	fbp ←	fructose‑1,6‑bisphosphatase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,455,281	0	T	C	100.0%	260.3 / NA	74	D110G (GAC→GGC)	fbp	fructose‑1,6‑bisphosphatase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/39);  total (35/39)

CCGCAACCTGTTTGTTACCAGGCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGT  >  NC_000913/4455144‑4455413                                                                                                                                          |                                                                                                                                     ccGCAACCTGTTTGTTACCAGGCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGccc                                                                                                                                    >  1:1169176/1‑140 (MQ=255)  cGCAACCTGTTTGTTACCAGGCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCa                                                                                                                                   >  2:941432/1‑140 (MQ=255)  cGCAACCTGTTTGTTACCAGGCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCa                                                                                                                                   >  2:1595941/1‑140 (MQ=255)   gCAACCTGTTTGTTACCAGGCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCAt                                                                                                                                  <  1:879656/140‑1 (MQ=255)   gCAACCTGTTTGTTACCAGGCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCAt                                                                                                                                  >  2:319360/1‑140 (MQ=255)     aaCCTGTTTGTTACCAGGCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCa                                                                                                                                <  2:365882/140‑1 (MQ=255)      aCCTGTTTGTTACCAGGCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAg                                                                                                                               <  2:547982/140‑1 (MQ=255)       ccTGTTTGTTACCAGGCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGc                                                                                                                              <  1:1340870/140‑1 (MQ=255)       ccTGTTTGTTACCAGGCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGc                                                                                                                              <  2:448619/140‑1 (MQ=255)           tttgttACCAGGCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGcacca                                                                                                                          <  2:1169176/140‑1 (MQ=255)           tttgttACCAGGCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGcacca                                                                                                                          >  2:230762/1‑140 (MQ=255)           tttgttACCAGGCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGcacca                                                                                                                          >  2:561430/1‑140 (MQ=255)                 aCCAGGCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCa                                                                                                                                >  1:762562/1‑128 (MQ=255)                 aCCAGGCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCa                                                                                                                                <  2:762562/128‑1 (MQ=255)                     ggCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCAt                                                                                                                                  >  1:522481/1‑122 (MQ=255)                     ggCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCAt                                                                                                                                  <  2:522481/122‑1 (MQ=255)                     ggCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGcacca                                                                                                                          >  2:1303211/1‑130 (MQ=255)                     ggCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGcacca                                                                                                                          <  1:1303211/130‑1 (MQ=255)                     ggCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGc                                                                                                                >  2:618088/1‑140 (MQ=255)                     ggCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGc                                                                                                                <  1:523191/140‑1 (MQ=255)                      gCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCg                                                                                                               >  2:1545329/1‑140 (MQ=255)                      gCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTCAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCg                                                                                                               <  2:746234/140‑1 (MQ=255)                          gaggaAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGtt                                                                                                           >  2:205446/1‑140 (MQ=255)                            ggaAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTca                                                                                                         >  1:287598/1‑140 (MQ=255)                            ggaAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTca                                                                                                         <  1:1595941/140‑1 (MQ=255)                             gaAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTcac                                                                                                        <  2:1212679/140‑1 (MQ=255)                              aaaTCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTcaca                                                                                                       <  2:799091/140‑1 (MQ=255)                              aaaTCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTcaca                                                                                                       <  1:957459/140‑1 (MQ=255)                              aaaTCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTcaca                                                                                                       <  2:147491/140‑1 (MQ=255)                               aaTCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAg                                                                                                      <  1:1545329/140‑1 (MQ=255)                                aTCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGc                                                                                                     >  1:415956/1‑140 (MQ=255)                                aTCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGc                                                                                                     >  2:1650279/1‑140 (MQ=255)                                aTCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGc                                                                                                     >  1:1028810/1‑140 (MQ=255)                                    tcttcCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTc                                                                                                 >  2:709938/1‑140 (MQ=255)                                     cttcCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCa                                                                                                >  1:1558619/1‑140 (MQ=255)                                     cttcCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCa                                                                                                >  1:248176/1‑140 (MQ=255)                                      ttcCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaa                                                                                               <  2:287598/140‑1 (MQ=255)                                           ttACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgacg                                                                                          >  2:117361/1‑140 (MQ=255)                                            tACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgacga                                                                                         <  1:616907/140‑1 (MQ=255)                                              ccGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACa                                                                                       <  2:889605/140‑1 (MQ=255)                                              ccGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACa                                                                                       >  1:1605332/1‑140 (MQ=255)                                              ccGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACa                                                                                       <  2:248176/140‑1 (MQ=255)                                                  cGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACa                                                                                       >  2:518498/1‑136 (MQ=255)                                                  cGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACa                                                                                       <  1:518498/136‑1 (MQ=255)                                                   gTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAAtctc                                                                                  <  2:977572/140‑1 (MQ=255)                                                   gTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGGATTTTGCGTGTTCACAGCCTTCAAAGACGACAAtctc                                                                                  >  2:722283/1‑140 (MQ=255)                                                    tGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCATAGCCTTCAAAGACGACAATCTCa                                                                                 <  2:1262968/140‑1 (MQ=255)                                                    tGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCa                                                                                 <  1:561430/140‑1 (MQ=255)                                                         aCAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttc                                                                            <  1:319360/140‑1 (MQ=255)                                                         aCAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttc                                                                            <  1:618088/140‑1 (MQ=255)                                                                      ggcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCa                                                               <  2:1164540/140‑1 (MQ=255)                                                                       gcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCaa                                                              >  2:1139020/1‑140 (MQ=255)                                                                        cggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAAt                                                             <  1:307280/140‑1 (MQ=255)                                                                         ggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATg                                                            <  2:1028810/140‑1 (MQ=255)                                                                           tAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGcc                                                          <  1:117361/140‑1 (MQ=255)                                                                           tAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGcc                                                          <  1:940846/140‑1 (MQ=255)                                                                                  agaAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACg                                                   >  1:1269676/1‑140 (MQ=255)                                                                                     aaaTGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGata                                                >  1:1207081/1‑140 (MQ=255)                                                                                        tGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATcg                                             <  1:1650279/140‑1 (MQ=255)                                                                                        tGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAACGCCCGCAACGATATcg                                             >  2:14799/1‑140 (MQ=255)                                                                                           tACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGt                                          >  1:1370640/1‑140 (MQ=255)                                                                                                aCAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCttt                                     >  1:1545856/1‑140 (MQ=255)                                                                                                    agaCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCCTGCTTTCAGt                                 >  2:1391761/1‑140 (MQ=255)                                                                                                     gaCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCCTGCTTTCAGTg                                <  1:1391761/140‑1 (MQ=255)                                                                                                       cGTTAACATCGATGTTGGGCGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCg                              >  2:1644181/1‑140 (MQ=255)                                                                                                          tAACATCGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCt                           <  2:1545856/140‑1 (MQ=255)                                                                                                                cGATGTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGt                     >  1:16367/1‑140 (MQ=255)                                                                                                                    gTTGGACGAGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGttttt                 <  2:415956/140‑1 (MQ=255)                                                                                                                            aGCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCg         <  2:16367/140‑1 (MQ=255)                                                                                                                             gCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGa        >  1:1352281/1‑140 (MQ=255)                                                                                                                             gCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGa        >  2:221285/1‑140 (MQ=255)                                                                                                                             gCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGa        >  2:925456/1‑140 (MQ=255)                                                                                                                             gCCATCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGa        <  2:1558619/140‑1 (MQ=255)                                                                                                                                 tCCAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACaa    >  2:1168651/1‑140 (MQ=255)                                                                                                                                  ccAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAg   >  2:257963/1‑140 (MQ=255)                                                                                                                                   cAGGGGGCCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAACGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGt  <  1:14799/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     CCGCAACCTGTTTGTTACCAGGCTGGAGGAAATCTTCTTCCGTTACCGGCGTGCCAACAGGCGTAACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGT  >  NC_000913/4455144‑4455413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑