Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,598,703	C→T	G247R (GGA→AGA)	opgB ←	phosphoglycerol transferase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,598,703	0	C	T	100.0%	331.2 / NA	92	G247R (GGA→AGA)	opgB	phosphoglycerol transferase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (48/44);  total (48/44)

CACGCTTTTCAGCTCTTCTGAGCCGTATAAGTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCCCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCGTGTAATCTGTTCCCGGCAGCTGCTGCGTG  >  NC_000913/4598569‑4598839                                                                                                                                       |                                                                                                                                         cACGCTTTTCAGCTCTTCTGAGCCGTATAAGTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTcagac                                                                                                                                     >  1:992959/1‑140 (MQ=255)  aCGCTTTTCAGCTCTTCTGAGCCGTATAAGTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTcagaca                                                                                                                                    >  1:1663090/1‑140 (MQ=255)  aCGCTTTTCAGCTCTTCTGAGCCGTATAAGTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTcagaca                                                                                                                                    <  2:1448353/140‑1 (MQ=255)       tttCAGCTCTTCTGAGCCGTATAAGTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGt                                                                                                                               >  2:1458424/1‑140 (MQ=255)                ttctGAGCCGTATAAGTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGga                                                                                                                      <  1:1446900/140‑1 (MQ=255)                  ctGAGCCGTATAAGTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGgaag                                                                                                                    >  1:214989/1‑140 (MQ=255)                  ctGAGCCGTATAAGTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGgaag                                                                                                                    <  2:992959/140‑1 (MQ=255)                  ctGAGCCGTATAAGTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGgaag                                                                                                                    <  2:214989/140‑1 (MQ=255)                       ccGTATAAGTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCt                                                                                                               >  1:636392/1‑140 (MQ=255)                         gTATAAGTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCTGACAGATGTTCTGCGGGAAGAAGCTgg                                                                                                             <  1:557887/140‑1 (MQ=255)                          tataAGTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGga                                                                                                            >  2:1413510/1‑140 (MQ=255)                          tataAGTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGga                                                                                                            >  1:1351588/1‑140 (MQ=255)                          tataAGTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGga                                                                                                            >  2:232142/1‑140 (MQ=255)                          tataAGTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGga                                                                                                            >  1:1310724/1‑140 (MQ=255)                              aGTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACa                                                                                                        <  1:381481/140‑1 (MQ=255)                               gTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACag                                                                                                       >  1:1382904/1‑140 (MQ=255)                                 ggTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACagag                                                                                                     >  1:728384/1‑140 (MQ=255)                                 ggTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACagag                                                                                                     >  1:421598/1‑140 (MQ=255)                                    cGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAgaggcg                                                                                                  >  1:733217/1‑140 (MQ=255)                                    cGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAgaggcg                                                                                                  >  2:28660/1‑140 (MQ=255)                                    cGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAgaggcg                                                                                                  >  2:160826/1‑140 (MQ=255)                                    cGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAgaggcg                                                                                                  >  2:1232898/1‑140 (MQ=255)                                    cGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAgaggcg                                                                                                  >  1:1244423/1‑140 (MQ=255)                                     gAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAgaggcgg                                                                                                 >  1:680369/1‑140 (MQ=255)                                     gAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAgaggcgg                                                                                                 >  1:192107/1‑140 (MQ=255)                                          cATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAgaggcggaggcg                                                                                            <  2:1147692/140‑1 (MQ=255)                                          cATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAgaggcggaggcg                                                                                            <  2:728384/140‑1 (MQ=255)                                          cATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAgaggcggaggcg                                                                                            <  1:1458424/140‑1 (MQ=255)                                          cATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAgaggcggaggcg                                                                                            <  2:1425986/140‑1 (MQ=255)                                           aTGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGt                                                                                           <  1:1232898/140‑1 (MQ=255)                                           aTGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGt                                                                                           <  2:1310724/140‑1 (MQ=255)                                           aTGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGt                                                                                           <  2:636392/140‑1 (MQ=255)                                            tGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAgaggcggaggcg                                                                                            >  2:998164/1‑138 (MQ=255)                                            tGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAgaggcggaggcg                                                                                            <  1:998164/138‑1 (MQ=255)                                            tGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGtt                                                                                          <  1:229801/140‑1 (MQ=255)                                                    cAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCaa                                                                                  <  2:1382904/140‑1 (MQ=255)                                                       gAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAgg                                                                               <  2:1225723/140‑1 (MQ=255)                                                         acacATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAgggg                                                                             <  2:192107/140‑1 (MQ=255)                                                            caTCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCa                                                                          >  1:899928/1‑140 (MQ=255)                                                             aTCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCaa                                                                         >  2:257210/1‑140 (MQ=255)                                                                 ttACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAg                                                                     >  2:1141638/1‑140 (MQ=255)                                                                     cGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGt                                                                 >  1:26390/1‑140 (MQ=255)                                                                        cAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATg                                                              <  1:279792/140‑1 (MQ=255)                                                                         aaaaCGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATg                                                              <  1:579031/139‑1 (MQ=255)                                                                         aaaaCGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATg                                                              >  2:579031/1‑139 (MQ=255)                                                                         aaaaCGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGc                                                             <  1:28660/140‑1 (MQ=255)                                                                            aCGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAg                                                                     <  2:1571093/129‑1 (MQ=255)                                                                            aCGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAg                                                                     >  1:1571093/1‑129 (MQ=255)                                                                               cAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACt                                                       <  2:692207/140‑1 (MQ=255)                                                                                     cgcgCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTg                                                      <  1:25011/135‑1 (MQ=255)                                                                                     cgcgCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTg                                                      >  2:25011/1‑135 (MQ=255)                                                                                          ccTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGccacc                                            <  1:1030317/140‑1 (MQ=255)                                                                                          ccTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGccacc                                            >  1:275184/1‑140 (MQ=255)                                                                                              cACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTga                                                     >  1:1499278/1‑127 (MQ=255)                                                                                              cACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTga                                                     <  2:1499278/127‑1 (MQ=255)                                                                                              cACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGc                                        >  2:243359/1‑140 (MQ=255)                                                                                              cACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGc                                        >  1:664411/1‑140 (MQ=255)                                                                                              cACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGc                                        >  2:1526937/1‑140 (MQ=255)                                                                                              cACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGc                                        >  1:212841/1‑140 (MQ=255)                                                                                               aCGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGcc                                       <  2:421598/140‑1 (MQ=255)                                                                                               aCGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGcc                                       <  1:1108855/140‑1 (MQ=255)                                                                                               aCGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGcc                                       <  2:1244423/140‑1 (MQ=255)                                                                                                 gAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAGGCCACCATGCCCg                                     <  2:1663090/140‑1 (MQ=255)                                                                                                   aaTAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCa                                   <  1:243359/140‑1 (MQ=255)                                                                                                   aaTAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCa                                   <  1:1353080/140‑1 (MQ=255)                                                                                                    aTAGTTCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCaa                                  <  1:1191336/140‑1 (MQ=255)                                                                                                        ttCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCgt                              >  1:1541474/1‑140 (MQ=255)                                                                                                        ttCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCgt                              >  2:390023/1‑140 (MQ=255)                                                                                                        ttCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCgt                              >  1:324723/1‑140 (MQ=255)                                                                                                        ttCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCgt                              >  2:167693/1‑140 (MQ=255)                                                                                                         tCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCa                                   >  2:772235/1‑134 (MQ=255)                                                                                                         tCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCa                                   <  1:772235/134‑1 (MQ=255)                                                                                                         tCTGATAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCgtg                             >  1:1424777/1‑140 (MQ=255)                                                                                                             aTAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCg                                     >  2:254756/1‑128 (MQ=255)                                                                                                             aTAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCg                                     <  1:254756/128‑1 (MQ=255)                                                                                                             aTAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCa                                   <  1:85805/130‑1 (MQ=255)                                                                                                             aTAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCa                                   >  2:85805/1‑130 (MQ=255)                                                                                                             aTAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCGTGTAAt                         <  2:733217/140‑1 (MQ=255)                                                                                                             aTAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCGTGTAAt                         >  1:1341535/1‑140 (MQ=255)                                                                                                             aTAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCGTGTAAt                         <  2:899928/140‑1 (MQ=255)                                                                                                             aTAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCGTGTAAt                         <  2:1541474/140‑1 (MQ=255)                                                                                                             aTAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCGTGTAAt                         >  2:1092011/1‑140 (MQ=255)                                                                                                             aTAACCCGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCGTGTAAt                         >  2:1425278/1‑140 (MQ=255)                                                                                                                 cccGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCGTGTAATCTGt                     >  2:1079779/1‑140 (MQ=255)                                                                                                                  ccGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAATCAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCGTGTAATCTGtt                    >  2:1644188/1‑140 (MQ=255)                                                                                                                  ccGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCg                                     <  1:855472/123‑1 (MQ=255)                                                                                                                  ccGAGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCg                                     >  2:855472/1‑123 (MQ=255)                                                                                                                     aGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCGTGTAATCTGTTccc                 <  2:26390/140‑1 (MQ=255)                                                                                                                     aGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCGTGTAATCTGTTccc                 >  2:896181/1‑140 (MQ=255)                                                                                                                     aGTTTTTCAGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCGTGTAATCTGTTccc                 <  1:1141638/140‑1 (MQ=255)                                                                                                                             aGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCGTGTAATCTGTTCCCGGCAgctg         <  2:680369/140‑1 (MQ=255)                                                                                                                             aGGATATCTCTCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCGTGTAATCTGTTCCCGGCAgctg         <  1:896181/140‑1 (MQ=255)                                                                                                                                    ctctCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCGTGTAATCTGTTCCCGGCAGCTGCTGCgtg  >  2:207484/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         CACGCTTTTCAGCTCTTCTGAGCCGTATAAGTGGTCGAAGCCATGCGACTTCAGGAACACATCTTTACCGGCAAAACGCAGATTCGCGCCCTGCACGAAATAGTTCTGATAACCCGAGTTTTTCAGGATATCTCCCAGACAGATGTTCTGCGGGAAGAAGCTGGAGACAGAGGCGGAGGCGTTGCCTTCAAAGGGGGCAAACAGCGGTATGCCGCACTGAGAAGCCACCATGCCCGCAATCGTGTAATCTGTTCCCGGCAGCTGCTGCGTG  >  NC_000913/4598569‑4598839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑