Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	435,252	A→G	D39G (GAT→GGT)	nusB →	transcription antitermination protein NusB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	435,252	0	A	G	100.0%	306.4 / NA	86	D39G (GAT→GGT)	nusB	transcription antitermination protein NusB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/41);  total (45/41)

AGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCA  >  NC_000913/435122‑435385                                                                                                                                    |                                                                                                                                      aGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGa                                                                                                                              >  1:1164856/1‑140 (MQ=255) aGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGa                                                                                                                              >  1:171129/1‑140 (MQ=255) aGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGa                                                                                                                              >  1:961622/1‑140 (MQ=255)      ggggAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTg                                                                                                                         >  1:970342/1‑140 (MQ=255)      ggggAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTg                                                                                                                         <  1:499204/140‑1 (MQ=255)       gggAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGa                                                                                                                        >  2:173281/1‑140 (MQ=255)         gAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACg                                                                                                                      <  1:372609/140‑1 (MQ=255)           aaTCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTc                                                                                                                    <  1:791639/140‑1 (MQ=255)            aTCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTg                                                                                                                         <  2:1604414/134‑1 (MQ=255)            aTCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTg                                                                                                                         >  1:1604414/1‑134 (MQ=255)            aTCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTcc                                                                                                                   >  1:1555743/1‑140 (MQ=255)                gTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTa                                                                                                               <  2:961622/140‑1 (MQ=255)                 tGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTAc                                                                                                              >  2:1342433/1‑140 (MQ=255)                 tGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTAc                                                                                                              >  1:1339763/1‑140 (MQ=255)                       ctgctgCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGt                                                                                                        >  2:1630542/1‑140 (MQ=255)                         gctgctCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACg                                                                                                                      >  2:627614/1‑124 (MQ=255)                         gctgctCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACg                                                                                                                      <  1:627614/124‑1 (MQ=255)                             ctCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAgctg                                                                                                  <  1:448956/140‑1 (MQ=255)                             ctCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAgctg                                                                                                  <  1:762297/140‑1 (MQ=255)                             ctCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAgctg                                                                                                  >  2:448956/1‑140 (MQ=255)                               cgtcgCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAgctgct                                                                                                >  1:1237568/1‑140 (MQ=255)                                gtcgCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGctgctg                                                                                               >  1:1420916/1‑140 (MQ=255)                                gtcgCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGctgctg                                                                                               >  1:618815/1‑140 (MQ=255)                                 tcgCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTgg                                                                                              <  1:173281/140‑1 (MQ=255)                                 tcgCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTgg                                                                                              >  2:1455090/1‑140 (MQ=255)                                  cgccgcGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGc                                                                                             >  1:24465/1‑140 (MQ=255)                                    ccgcGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCg                                                                                           >  1:690124/1‑140 (MQ=255)                                     cgcgCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCgg                                                                                          >  2:683869/1‑140 (MQ=255)                                       cgcTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCgggg                                                                                        <  2:1164856/140‑1 (MQ=255)                                         cTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGta                                                                                      <  1:1630542/140‑2 (MQ=255)                                         cTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTg                                                                                      <  1:1660052/140‑1 (MQ=255)                                         cTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACAACGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTg                                                                                      <  2:709916/140‑1 (MQ=255)                                           cGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGc                                                                                    <  1:1024068/140‑1 (MQ=255)                                           cGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGc                                                                                    >  1:385649/1‑140 (MQ=255)                                              gAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGAc                                                                                 <  2:171129/140‑1 (MQ=255)                                                gtgtGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTa                                                                               <  2:618815/140‑1 (MQ=255)                                                 tgtgCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTaa                                                                              >  2:1436557/1‑140 (MQ=255)                                                   tgCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTg                                                                                      >  2:1026747/1‑130 (MQ=255)                                                   tgCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTg                                                                                      <  1:1026747/130‑1 (MQ=255)                                                   tgCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATa                                                                            >  2:693281/1‑140 (MQ=255)                                                    gCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTg                                                                                      <  2:251723/129‑1 (MQ=255)                                                    gCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTg                                                                                      >  1:251723/1‑129 (MQ=255)                                                       gTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGc                                                                        >  1:1486840/1‑140 (MQ=255)                                                           aGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATAc                                                                    <  2:1555743/140‑1 (MQ=255)                                                                  ctACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACg                                                             >  1:750677/1‑140 (MQ=255)                                                                      tCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACt                                                         <  1:1342433/140‑1 (MQ=255)                                                                        cTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACtga                                                       <  2:1420916/140‑1 (MQ=255)                                                                         tGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACtgat                                                      <  2:750677/140‑1 (MQ=255)                                                                           gCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACtgatga                                                    >  2:981783/1‑140 (MQ=255)                                                                           gCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACtgatga                                                    >  1:2652/1‑140 (MQ=255)                                                                              gTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGc                                                 <  1:1436557/140‑1 (MQ=255)                                                                               ttGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGc                                                 >  2:567126/1‑140 (MQ=255)                                                                               ttGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGcc                                                >  1:315978/1‑140 (MQ=255)                                                                               ttGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGcc                                                >  2:456832/1‑140 (MQ=255)                                                                               ttGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGcc                                                >  1:577738/1‑140 (MQ=255)                                                                                  tCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATa                                             <  1:683869/140‑1 (MQ=255)                                                                                         aCGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTcc                                      >  2:215784/1‑140 (MQ=255)                                                                                            aCATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGc                                   <  2:1486840/140‑1 (MQ=255)                                                                                            aCATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGc                                   >  2:876594/1‑140 (MQ=255)                                                                                            aCATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGc                                   >  2:208665/1‑140 (MQ=255)                                                                                             cATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGcc                                  >  2:1072641/1‑140 (MQ=255)                                                                                               tCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCctg                                <  1:456832/140‑1 (MQ=255)                                                                                                cGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGc                                                 >  2:1208830/1‑122 (MQ=255)                                                                                                cGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGc                                                 <  1:1208830/122‑1 (MQ=255)                                                                                                cGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCctgc                               <  2:24465/140‑1 (MQ=255)                                                                                                  cTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCctgctg                             >  2:557009/1‑140 (MQ=255)                                                                                                   tGATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTgg                            >  1:585215/1‑140 (MQ=255)                                                                                                    gATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGga                           <  1:215784/140‑1 (MQ=255)                                                                                                    gATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGga                           <  2:585215/140‑1 (MQ=255)                                                                                                    gATGTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGga                           <  2:315978/140‑1 (MQ=255)                                                                                                       gTTGAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGgaaga                        >  2:1453711/1‑140 (MQ=255)                                                                                                          gAATACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGT‑CTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTg                    >  1:1330267/1‑140 (MQ=255)                                                                                                            aTACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTgg                   <  1:872508/140‑1 (MQ=255)                                                                                                             tACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTggg                  >  2:300618/1‑140 (MQ=255)                                                                                                             tACCAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGT‑CTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGa                 <  2:1330267/140‑1 (MQ=255)                                                                                                               ccggTTCCTGGGTGAAAA‑GGGGGTAAAAGACGTTGACGCCCTGTACTTCCGTGAGCTGCGGGCGGGGGTGGCGGCTAAAACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGAc                <  2:970342/137‑1 (MQ=255)                                                                                                               ccAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGga                           <  1:1543924/129‑1 (MQ=255)                                                                                                               ccAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGga                           >  2:1543924/1‑129 (MQ=255)                                                                                                                cAGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACa               <  2:1638156/140‑1 (MQ=255)                                                                                                                 aGTTCCTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAg              <  1:1455090/140‑1 (MQ=255)                                                                                                                      cTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGa         <  2:690124/140‑1 (MQ=255)                                                                                                                      cTGGCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGa         <  1:751139/140‑1 (MQ=255)                                                                                                                         gCTGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGaaaa      >  1:872144/1‑140 (MQ=255)                                                                                                                           tGAACA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAg    <  1:981783/140‑1 (MQ=255)                                                                                                                             aaCA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCa  <  2:311395/140‑1 (MQ=255)                                                                                                                             aaCA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCa  <  1:557009/140‑1 (MQ=255)                                                                                                                             aaCA‑GGGTGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCa  >  1:311395/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                      AGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACA‑GGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCA  >  NC_000913/435122‑435385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑