Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	441,683	C→T	R45R (CGC→CGT)	thiI →	tRNA uridine 4‑sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	441,683	0	C	T	100.0%	280.5 / NA	78	R45R (CGC→CGT)	thiI	tRNA uridine 4‑sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (47/31);  total (47/31)

CCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTT  >  NC_000913/441547‑441819                                                                                                                                         |                                                                                                                                         ccATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCAc                                                                                                                                       <  1:1389788/140‑1 (MQ=255)    tGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTgg                                                                                                                                    <  1:338359/140‑1 (MQ=255)                 tAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAg                                                                                                                       >  2:1251021/1‑140 (MQ=255)                                     ccATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAAc                                                                                                   <  1:1050750/140‑1 (MQ=255)                                      cATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAAcc                                                                                                  <  2:1331591/140‑1 (MQ=255)                                        tCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAg                                                                                                >  2:1311458/1‑140 (MQ=255)                                                   tCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTAt                                                                                     >  1:331657/1‑140 (MQ=255)                                                   tCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTAt                                                                                     >  1:516836/1‑140 (MQ=255)                                                        gcgcttgcgcttTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTcgcg                                                                                <  2:1065970/140‑1 (MQ=255)                                                             tgcgcttTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCACAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGct                                                                           >  1:827143/1‑140 (MQ=255)                                                               cgcttTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGctct                                                                         <  1:1311458/140‑1 (MQ=255)                                                               cgcttTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGctct                                                                         <  1:649251/140‑1 (MQ=255)                                                                 cttTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGa                                                                       >  1:1418891/1‑140 (MQ=255)                                                                   ttATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGAcc                                                                     <  1:711635/140‑1 (MQ=255)                                                                   ttATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGAcc                                                                     <  2:1498346/140‑1 (MQ=255)                                                                   ttATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGAcc                                                                     <  2:331657/140‑1 (MQ=255)                                                                      taAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTcgcg                                                                                <  2:895781/126‑1 (MQ=255)                                                                      taAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTcgcg                                                                                >  1:895781/1‑126 (MQ=255)                                                                           aTCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTcc                                                             >  1:1619218/1‑140 (MQ=255)                                                                                tACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTc                                                              >  2:222931/1‑134 (MQ=255)                                                                                tACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTc                                                              <  1:222931/134‑1 (MQ=255)                                                                                tACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGt                                                         <  1:1485756/139‑1 (MQ=255)                                                                                tACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTa                                                        >  1:410132/1‑140 (MQ=255)                                                                                tACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTa                                                        >  1:665097/1‑140 (MQ=255)                                                                                tACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACATGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGt                                                         >  2:1485756/1‑139 (MQ=255)                                                                                 aCCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCACAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTAt                                                       <  2:827143/140‑1 (MQ=255)                                                                                     ggAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATccac                                                   >  1:1327034/1‑140 (MQ=255)                                                                                      gAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATccacc                                                  <  1:483403/140‑1 (MQ=255)                                                                                       aaCATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATccacca                                                 >  1:577223/1‑140 (MQ=255)                                                                                       aaCATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATc                                                      <  1:1481208/135‑1 (MQ=255)                                                                                       aaCATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATc                                                      >  2:1481208/1‑135 (MQ=255)                                                                                             cGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATtct                                           >  2:1135783/1‑140 (MQ=255)                                                                                               tAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCtcg                                         <  1:1135783/140‑1 (MQ=255)                                                                                                aaCGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGGTAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCtcga                                        >  2:1001364/1‑140 (MQ=255)                                                                                                aaCGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCtcga                                        >  1:1214751/1‑140 (MQ=255)                                                                                                     tttAAAGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCtcgaagtcg                                   >  1:1457946/1‑140 (MQ=255)                                                                                                          aGCACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGAc                              <  1:518306/140‑1 (MQ=255)                                                                                                            cACTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGt                            <  2:577223/140‑1 (MQ=255)                                                                                                              cTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCGGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGc                          >  2:1554151/1‑140 (MQ=255)                                                                                                              cTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGc                          >  1:535768/1‑140 (MQ=255)                                                                                                              cTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGc                          >  2:1291558/1‑140 (MQ=255)                                                                                                              cTATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGc                          >  2:1629629/1‑140 (MQ=255)                                                                                                               tATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCGTATTCTCGAAGTCGAAGACGTGcc                         >  2:525733/1‑140 (MQ=255)                                                                                                               tATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGcc                         <  2:516836/140‑1 (MQ=255)                                                                                                               tATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGcc                         >  2:626976/1‑140 (MQ=255)                                                                                                               tATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGcc                         <  1:1610946/140‑1 (MQ=255)                                                                                                               tATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGcc                         >  1:1048100/1‑140 (MQ=255)                                                                                                               tATGATGAGACGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGcc                         >  2:674028/1‑140 (MQ=255)                                                                                                                      agaCGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTAcc                  >  1:1252656/1‑140 (MQ=255)                                                                                                                      agaCGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTAcc                  >  2:15404/1‑140 (MQ=255)                                                                                                                      agaCGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTAcc                  >  2:298970/1‑140 (MQ=255)                                                                                                                       gaCGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCg                 >  2:379525/1‑140 (MQ=255)                                                                                                                        aCGCTCGCTGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGa                >  1:252990/1‑140 (MQ=255)                                                                                                                         cgctcgctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCtcgaagtcgaag                                >  2:1204974/1‑123 (MQ=255)                                                                                                                         cgctcgctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCtcgaagtcgaag                                <  1:1204974/123‑1 (MQ=255)                                                                                                                         cgctcgctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTg                           <  2:1254812/128‑1 (MQ=255)                                                                                                                         cgctcgctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTg                           >  1:1254812/1‑128 (MQ=255)                                                                                                                         cgctcgctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGAc               >  1:1436300/1‑140 (MQ=255)                                                                                                                         cgctcgctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGAc               >  2:1165583/1‑140 (MQ=255)                                                                                                                         cgctcgctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGAc               >  2:1116360/1‑140 (MQ=255)                                                                                                                           ctcgctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGAc               <  2:557396/138‑1 (MQ=255)                                                                                                                           ctcgctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGAc               >  1:557396/1‑138 (MQ=255)                                                                                                                           ctcgctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACAt             >  2:1204999/1‑140 (MQ=255)                                                                                                                           ctcgctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACAt             >  1:995079/1‑140 (MQ=255)                                                                                                                           ctcgctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACAt             >  1:836910/1‑140 (MQ=255)                                                                                                                           ctcgctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACAt             >  1:519385/1‑140 (MQ=255)                                                                                                                           ctcgctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACAt             >  1:715202/1‑140 (MQ=255)                                                                                                                            tcgctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATg            <  2:836910/140‑1 (MQ=255)                                                                                                                            tcgctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATg            <  1:15404/140‑1 (MQ=255)                                                                                                                            tcgctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATg            >  1:1336402/1‑140 (MQ=255)                                                                                                                            tcgctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATg            <  1:674028/140‑1 (MQ=255)                                                                                                                             cgctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGc           >  2:404515/1‑140 (MQ=255)                                                                                                                             cgctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGc           >  1:1431242/1‑140 (MQ=255)                                                                                                                              gctGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCa          <  2:1327034/140‑1 (MQ=255)                                                                                                                                tGTCGTCCGTCACTGGGGTAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACg        <  1:1001364/140‑1 (MQ=255)                                                                                                                                tGTCGTCCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACg        >  2:1433052/1‑140 (MQ=255)                                                                                                                                  tcgtcCGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGat      <  1:1204999/140‑1 (MQ=255)                                                                                                                                      ccGTCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATAttt  <  2:252990/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTT  >  NC_000913/441547‑441819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑