Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	684,998	G→A	F86F (TTC→TTT)	gltL ←	glutamate/aspartate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	684,998	0	G	A	100.0%	307.0 / NA	85	F86F (TTC→TTT)	gltL	glutamate/aspartate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/43);  total (42/43)

ATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGT  >  NC_000913/684863‑685132                                                                                                                                        |                                                                                                                                       aTTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAAt                                                                                                                                    <  1:199195/140‑1 (MQ=255)   tGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCCTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGc                                                                                                                                  <  2:257774/140‑1 (MQ=255)        tGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGaa                                                                                                                             <  2:1646237/140‑1 (MQ=255)         gAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGc                                                                                                                                  <  2:1604673/134‑1 (MQ=255)         gAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGc                                                                                                                                  >  1:1604673/1‑134 (MQ=255)          aGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATa                                                                                                                           <  2:181380/140‑1 (MQ=255)          aGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATa                                                                                                                           >  1:1519154/1‑140 (MQ=255)               aaaGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATa                                                                                                                           <  1:1089852/135‑1 (MQ=255)               aaaGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATa                                                                                                                           >  2:1089852/1‑135 (MQ=255)               aaaGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATc                                                                                                                      >  1:1170791/1‑140 (MQ=255)               aaaGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATc                                                                                                                      >  1:215898/1‑140 (MQ=255)                aaGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATcc                                                                                                                     >  2:289396/1‑140 (MQ=255)                   cccGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGa                                                                                                                  <  2:1623623/140‑1 (MQ=255)                        cacGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCgg                                                                                                             <  1:289396/140‑1 (MQ=255)                           gCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGaa                                                                                                          <  2:505902/140‑1 (MQ=255)                            cTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAAc                                                                                                         <  2:419071/140‑1 (MQ=255)                             tCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACg                                                                                                        >  2:342634/1‑140 (MQ=255)                             tCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACg                                                                                                        <  2:1519154/140‑1 (MQ=255)                             tCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACg                                                                                                        >  2:1543904/1‑140 (MQ=255)                                    gTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCttt                                                                                                 <  2:1380818/140‑1 (MQ=255)                                     tttCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTg                                                                                                >  2:1392948/1‑140 (MQ=255)                                     tttCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTg                                                                                                >  1:667132/1‑140 (MQ=255)                                      ttCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGc                                                                                               <  1:1425874/140‑1 (MQ=255)                                       tCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGcc                                                                                              >  2:710323/1‑140 (MQ=255)                                        cAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCa                                                                                             <  1:1498202/140‑1 (MQ=255)                                         aGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAg                                                                                            >  1:169254/1‑140 (MQ=255)                                           ggCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGAt                                                                                          <  2:667132/140‑1 (MQ=255)                                            gCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAg                                                                                            <  2:994013/137‑1 (MQ=255)                                            gCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAg                                                                                            >  1:994013/1‑137 (MQ=255)                                            gCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATc                                                                                         >  2:1610020/1‑140 (MQ=255)                                             cTTTTTCACGAGCCGGTGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGa                                                                                           >  2:941847/1‑137 (MQ=255)                                             cTTTTTCACGAGCCGGTGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGa                                                                                           <  1:941847/137‑1 (MQ=255)                                               ttttCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGt                                                                                      >  2:966863/1‑140 (MQ=255)                                               ttttCACGAGCCGGCGCTTTATCGCGTTTAACCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGt                                                                                      >  2:1192311/1‑140 (MQ=255)                                                tttCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGtt                                                                                     >  2:1601991/1‑140 (MQ=255)                                                         ccGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCg                                                                            <  1:263343/140‑1 (MQ=255)                                                           ggCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGtt                                                                          <  1:710323/140‑1 (MQ=255)                                                           ggCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGtt                                                                          <  1:662377/140‑1 (MQ=255)                                                            gcgcTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTa                                                                         >  1:755930/1‑140 (MQ=255)                                                            gcgcTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTa                                                                         <  1:1610020/140‑1 (MQ=255)                                                              gcTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTCTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAAc                                                                       <  2:1544573/140‑1 (MQ=255)                                                                    tCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGAt                                                                 <  1:710919/140‑1 (MQ=255)                                                                          ttAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATc                                                           <  1:1658842/140‑1 (MQ=255)                                                                             aGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGAc                                                        <  1:342634/140‑1 (MQ=255)                                                                              gCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATc                                                           >  2:907491/1‑136 (MQ=255)                                                                              gCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATc                                                           <  1:907491/136‑1 (MQ=255)                                                                               cACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACgg                                                      <  2:755930/140‑1 (MQ=255)                                                                                  tttCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGa                                                   >  2:237809/1‑140 (MQ=255)                                                                                   ttCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGAt                                                  >  1:208571/1‑140 (MQ=255)                                                                                    tCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGAtt                                                 >  2:784563/1‑140 (MQ=255)                                                                                      aCCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTc                                               >  1:680266/1‑140 (MQ=255)                                                                                       ccTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCa                                              >  1:537373/1‑140 (MQ=255)                                                                                       ccTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCa                                              <  2:1170791/140‑1 (MQ=255)                                                                                        cTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCAc                                             <  1:237809/140‑1 (MQ=255)                                                                                         tGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCAc                                             <  2:277022/139‑1 (MQ=255)                                                                                         tGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCAc                                             >  1:277022/1‑139 (MQ=255)                                                                                          gcgcCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCt                                           >  2:1283481/1‑140 (MQ=255)                                                                                          gcgcCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCt                                           <  2:865476/140‑1 (MQ=255)                                                                                           cgcCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCtt                                          >  1:10116/1‑140 (MQ=255)                                                                                            gcCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCTGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTtg                                         <  1:440281/140‑1 (MQ=255)                                                                                            gcCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTtg                                         >  2:966927/1‑140 (MQ=255)                                                                                            gcCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTtg                                         >  2:40796/1‑140 (MQ=255)                                                                                            gcCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTtg                                         >  2:567982/1‑140 (MQ=255)                                                                                                 ggTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCa                                    <  1:919168/140‑1 (MQ=255)                                                                                                  gTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCAc                                   <  1:966927/140‑1 (MQ=255)                                                                                                  gTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCAc                                   <  2:668399/140‑1 (MQ=255)                                                                                                         tttCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCg                            <  1:1192311/140‑1 (MQ=255)                                                                                                           tCGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAg                          >  1:1263642/1‑140 (MQ=255)                                                                                                            cGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAgg                         <  1:1392948/140‑1 (MQ=255)                                                                                                            cGATAATCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAgg                         >  2:778196/1‑140 (MQ=255)                                                                                                                 aTCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGtt                    >  1:902152/1‑140 (MQ=255)                                                                                                                  tCGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTg                   >  1:584795/1‑140 (MQ=255)                                                                                                                   cGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGa                  <  2:169254/140‑1 (MQ=255)                                                                                                                   cGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGa                  <  2:1263642/140‑1 (MQ=255)                                                                                                                   cGACAGATGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGa                  <  2:663612/140‑1 (MQ=255)                                                                                                                         aTGAGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGttt            >  2:181103/1‑140 (MQ=255)                                                                                                                            aGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTaa         >  2:754699/1‑140 (MQ=255)                                                                                                                            aGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTaa         >  2:1384391/1‑140 (MQ=255)                                                                                                                            aGGGAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGATTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTaa         >  1:148311/1‑140 (MQ=255)                                                                                                                              ggAACAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATc       >  1:122243/1‑140 (MQ=255)                                                                                                                                 aCAGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGc    <  1:1601991/140‑1 (MQ=255)                                                                                                                                   aGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGa                  >  2:822643/1‑124 (MQ=255)                                                                                                                                   aGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGa                  <  1:822643/124‑1 (MQ=255)                                                                                                                                   aGCTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGt  >  2:575651/1‑140 (MQ=255)                                                                                                                                     cTCAAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAAt        <  2:1334840/132‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       ATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGT  >  NC_000913/684863‑685132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑