Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	700,681	T→C	N305S (AAC→AGC)	nagC ←	DNA‑binding transcriptional dual regulator NagC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	700,681	0	T	C	100.0%	267.7 / NA	75	N305S (AAC→AGC)	nagC	DNA‑binding transcriptional dual regulator NagC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/32);  total (43/32)

CACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGC  >  NC_000913/700545‑700816                                                                                                                                         |                                                                                                                                        caCTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTcg                                                                                                                                      >  1:256253/1‑140 (MQ=255)    tttATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCgg                                                                                                                                   >  1:1156766/1‑140 (MQ=255)      tATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCt                                                                                                                                 >  2:843748/1‑140 (MQ=255)         cGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTg                                                                                                                              >  2:384411/1‑140 (MQ=255)           gCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCa                                                                                                                            <  1:1150484/140‑1 (MQ=255)           gCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCa                                                                                                                            >  1:1637798/1‑140 (MQ=255)             ttCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGa                                                                                                                          >  1:910425/1‑140 (MQ=255)              tCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGa                                                                                                                          >  2:666395/1‑139 (MQ=255)              tCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGa                                                                                                                          <  1:666395/139‑1 (MQ=255)              tCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGAt                                                                                                                         >  1:1096736/1‑140 (MQ=255)                ggTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAg                                                                                                                       >  1:257223/1‑140 (MQ=255)                      ttCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGa                                                                                                                 >  2:394741/1‑140 (MQ=255)                            ggCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGc                                                                                                           >  1:203485/1‑140 (MQ=255)                                           ttGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCa                                                                                            <  2:1637798/140‑1 (MQ=255)                                             gCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCAcg                                                                                          <  1:843748/140‑1 (MQ=255)                                             gCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCAcg                                                                                          <  2:203485/140‑1 (MQ=255)                                                 aTTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCg                                                                                               <  1:1547417/131‑1 (MQ=255)                                                 aTTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCg                                                                                               >  2:1547417/1‑131 (MQ=255)                                                 aTTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGc                                                                                      <  1:394741/140‑1 (MQ=255)                                                 aTTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGc                                                                                      <  2:100967/140‑1 (MQ=255)                                                      aTAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTgg                                                                                 <  2:1096736/140‑1 (MQ=255)                                                           ttGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGc                                                                                      >  1:928095/1‑130 (MQ=255)                                                           ttGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGc                                                                                      <  2:928095/130‑1 (MQ=255)                                                           ttGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGcc                                                                            <  2:926304/140‑1 (MQ=255)                                                           ttGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGcc                                                                            >  1:1549372/1‑140 (MQ=255)                                                           ttGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGcc                                                                            <  2:257223/140‑1 (MQ=255)                                                             gATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGccc                                                                           >  2:1481052/1‑139 (MQ=255)                                                             gATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGccc                                                                           <  1:1481052/139‑1 (MQ=255)                                                             gATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCt                                                                          >  2:319700/1‑140 (MQ=255)                                                             gATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCt                                                                          >  1:921932/1‑140 (MQ=255)                                                              aTAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTg                                                                         <  2:300935/140‑1 (MQ=255)                                                               tAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATGGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGc                                                                        >  1:1374706/1‑140 (MQ=255)                                                               tAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGc                                                                        >  1:1384730/1‑140 (MQ=255)                                                               tAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGc                                                                        >  2:1635982/1‑140 (MQ=255)                                                                aGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCt                                                                       <  1:1557152/140‑1 (MQ=255)                                                                    aTGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTaa                                                                   >  1:1160777/1‑140 (MQ=255)                                                                     tGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAAc                                                                  <  2:910425/140‑1 (MQ=255)                                                                       gCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAg                                                                >  2:609859/1‑140 (MQ=255)                                                                        cGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGa                                                               >  1:5938/1‑140 (MQ=255)                                                                          aTGGTTTTACCAAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGAtt                                                             >  1:1521688/1‑140 (MQ=255)                                                                              ttttACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAac                                                         <  2:596389/140‑1 (MQ=255)                                                                              ttttACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAac                                                         <  1:609859/140‑1 (MQ=255)                                                                                 tACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGAt                                                              >  2:432733/1‑132 (MQ=255)                                                                                 tACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGAt                                                              <  1:432733/132‑1 (MQ=255)                                                                                 tACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACAcc                                                      >  1:1198772/1‑140 (MQ=255)                                                                                   cccAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCg                                                    <  2:1198772/140‑1 (MQ=255)                                                                                   cccAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCg                                                    <  1:42025/140‑1 (MQ=255)                                                                                    ccAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCgt                                                   <  1:1225514/140‑1 (MQ=255)                                                                                       gATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACa                                                                 <  2:696539/123‑1 (MQ=255)                                                                                       gATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACa                                                                 >  1:696539/1‑123 (MQ=255)                                                                                        aTGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTTCTTTAACAGATTCAACACCCg                                                    >  2:811785/1‑135 (MQ=255)                                                                                        aTGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTTCTTTAACAGATTCAACACCCg                                                    <  1:811785/135‑1 (MQ=255)                                                                                        aTGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCgttgtt                                               >  2:211278/1‑140 (MQ=255)                                                                                        aTGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCgttgtt                                               <  2:1384730/140‑1 (MQ=255)                                                                                              accgacATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATgg                                         >  2:1189410/1‑140 (MQ=255)                                                                                              accgacATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATgg                                         <  1:1550161/140‑1 (MQ=255)                                                                                              accgacATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATgg                                         >  1:76923/1‑140 (MQ=255)                                                                                                  acATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGc                                     >  1:593515/1‑140 (MQ=255)                                                                                                          aaTTACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATGGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGTAGCGTTGGCAg                             <  2:1374706/140‑1 (MQ=255)                                                                                                            ttACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGtt                                  <  2:780598/133‑1 (MQ=255)                                                                                                            ttACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGtt                                  >  1:780598/1‑133 (MQ=255)                                                                                                            ttACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCg                           >  1:1553893/1‑140 (MQ=255)                                                                                                             tACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGa                          >  1:679930/1‑140 (MQ=255)                                                                                                             tACTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGa                          >  2:1006544/1‑140 (MQ=255)                                                                                                              aCTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGAt                         <  1:211278/140‑1 (MQ=255)                                                                                                              aCTTCCGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGAt                         <  1:1006544/140‑1 (MQ=255)                                                                                                                  ccGACGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGtt                     >  2:301511/1‑140 (MQ=255)                                                                                                                    gACGCCAGACTATCGCCTTCGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTc                   >  1:1158398/1‑140 (MQ=255)                                                                                                                     aCGCCAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTcc                  >  1:721325/1‑140 (MQ=255)                                                                                                                         cAGACTATCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGc              >  2:640957/1‑140 (MQ=255)                                                                                                                               aTCGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCaa        >  1:954537/1‑140 (MQ=255)                                                                                                                                 cGCCTTTGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAg      >  2:771340/1‑140 (MQ=255)                                                                                                                                   ccTTCGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGtt    <  2:1158398/140‑1 (MQ=255)                                                                                                                                     tttGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGc  <  2:721325/140‑1 (MQ=255)                                                                                                                                     tttGCTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGc  >  2:421149/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGC  >  NC_000913/700545‑700816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑