Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	913,963	C→T	A299T (GCG→ACG)	lysO ←	L‑lysine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	913,963	0	C	T	98.9%	314.2 / NA	93	A299T (GCG→ACG)	lysO	L‑lysine exporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (51/41);  minor base A (0/1);  total (51/42)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTATATTTAATATACATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGCAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTGAAGAACGGGCAGGGTGAAATCCATTGATGTGGCACCGCATAA  >  NC_000913/913831‑914098                                                                                                                                     |                                                                                                                                        gTTATATTTAATATACATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGaaa                                                                                                                                  <  2:156797/140‑1 (MQ=255)   tataTTTAATATACATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGaaaaa                                                                                                                                >  2:563387/1‑140 (MQ=255)   tataTTTAATATACATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGaaaaa                                                                                                                                >  1:1127194/1‑140 (MQ=255)   tataTTTAATATACATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGaaaaa                                                                                                                                >  2:69821/1‑140 (MQ=255)   tataTTTAATATACATGTTTAAGGTTAAGACGCTTAACGCGGGGAAAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGACGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGaaaaa                                                                                                                                >  2:1144613/1‑140 (MQ=255)    atatTTAATATACATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAg                                                                                                                               >  2:577792/1‑140 (MQ=255)    atatTTAATATACATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAg                                                                                                                               <  1:577792/140‑1 (MQ=255)     tatTTAATATACATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAgg                                                                                                                              >  1:298148/1‑140 (MQ=255)     tatTTAATATACATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAgg                                                                                                                              >  2:194418/1‑140 (MQ=255)            tataCATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAg                                                                                                                       >  2:919485/1‑140 (MQ=255)            tataCATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAg                                                                                                                       >  1:1540505/1‑140 (MQ=255)              taCATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGa                                                                                                                     <  2:1540505/140‑1 (MQ=255)              taCATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGa                                                                                                                     <  2:240459/140‑1 (MQ=255)              taCATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGa                                                                                                                     <  1:194418/140‑1 (MQ=255)               aCATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGAt                                                                                                                    >  1:242097/1‑140 (MQ=255)               aCATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGAt                                                                                                                    >  1:1627070/1‑140 (MQ=255)               aCATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGAt                                                                                                                    <  1:525803/140‑1 (MQ=255)               aCATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGAt                                                                                                                    <  1:69821/140‑1 (MQ=255)                cATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATc                                                                                                                   >  1:229659/1‑140 (MQ=255)                cATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATc                                                                                                                   >  2:1030235/1‑140 (MQ=255)                 aTGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTCGAGAAAAAGGCGATGAGGATc                                                                                                                   <  2:1181721/139‑1 (MQ=255)                 aTGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATc                                                                                                                   >  1:1181721/1‑139 (MQ=255)                 aTGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCg                                                                                                                  >  2:690520/1‑140 (MQ=255)                 aTGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCg                                                                                                                  >  1:1041779/1‑140 (MQ=255)                   gTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATg                                                                                                                         >  2:352194/1‑131 (MQ=255)                   gTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATg                                                                                                                         >  2:60434/1‑131 (MQ=255)                   gTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATg                                                                                                                         <  1:352194/131‑1 (MQ=255)                   gTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATg                                                                                                                         <  1:60434/131‑1 (MQ=255)                   gTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGa                                                                                                                     >  2:101382/1‑135 (MQ=255)                   gTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGa                                                                                                                     <  1:101382/135‑1 (MQ=255)                   gTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGc                                                                                                                <  1:1316446/140‑1 (MQ=255)                      tAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATg                                                                                                                         <  2:1055714/128‑1 (MQ=255)                      tAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATg                                                                                                                         >  1:1055714/1‑128 (MQ=255)                      tAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACt                                                                                                             >  2:841185/1‑140 (MQ=255)                      tAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACt                                                                                                             >  1:1138006/1‑140 (MQ=255)                        aGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTaa                                                                                                           >  1:60997/1‑140 (MQ=255)                         ggTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAAc                                                                                                          <  1:1144613/140‑1 (MQ=255)                           ttAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAg                                                                                                        >  2:675857/1‑140 (MQ=255)                            tAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAgg                                                                                                       <  2:664587/140‑1 (MQ=255)                            tAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAgg                                                                                                       <  1:503782/140‑1 (MQ=255)                              aGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGa                                                                                                                     <  2:1227306/124‑1 (MQ=255)                              aGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGa                                                                                                                     >  1:1227306/1‑124 (MQ=255)                              aGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCTCTATCAGGCt                                                                                                     <  2:60997/140‑1 (MQ=255)                              aGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCt                                                                                                     <  1:1030235/140‑1 (MQ=255)                              aGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCt                                                                                                     <  2:1138006/140‑1 (MQ=255)                                   cTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCgg                                                                                                                 >  2:291430/1‑123 (MQ=255)                                   cTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCgg                                                                                                                 <  1:291430/123‑1 (MQ=255)                                    ttAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCa                                                                                                               >  1:812323/1‑124 (MQ=255)                                    ttAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCa                                                                                                               <  2:812323/124‑1 (MQ=255)                                    ttAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAAt                                                                                               >  1:1076756/1‑140 (MQ=255)                                    ttAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAAt                                                                                               >  2:792979/1‑140 (MQ=255)                                    ttAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGAATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAAt                                                                                               >  2:964058/1‑140 (MQ=255)                                     tAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCt                                                                                                     <  1:1022498/133‑1 (MQ=255)                                     tAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCt                                                                                                     >  2:1022498/1‑133 (MQ=255)                                             ggATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTg                                                                                      >  1:1156186/1‑140 (MQ=255)                                                        aTTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTgccg                                                                           >  2:761948/1‑140 (MQ=255)                                                         tttttCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTgccgc                                                                          <  2:665359/140‑1 (MQ=255)                                                         tttttCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTgccgc                                                                          >  1:211674/1‑140 (MQ=255)                                                          ttttCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTgccgcg                                                                         <  1:690520/140‑2 (MQ=255)                                                           tttCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGccgccg                                                                        <  2:1076756/140‑1 (MQ=255)                                                           tttCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGccgccg                                                                        <  1:761948/140‑1 (MQ=255)                                                           tttCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGccgccg                                                                        <  2:229659/140‑1 (MQ=255)                                                                          aGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGCCCATATCCAGcc                                                         <  1:995363/140‑1 (MQ=255)                                                                               gattgattTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCa                                                    >  1:684035/1‑140 (MQ=255)                                                                               gattgattTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCa                                                    >  2:353987/1‑140 (MQ=255)                                                                                attgattTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGGACAATTGCCGCCGGGACCATATCCAGCCCGCCAg                                                   >  2:691892/1‑140 (MQ=255)                                                                                attgattTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAg                                                   >  1:681049/1‑140 (MQ=255)                                                                                attgattTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAg                                                   >  2:1419342/1‑140 (MQ=255)                                                                                 ttgattTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGt                                                  <  2:684035/140‑1 (MQ=255)                                                                                 ttgattTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGt                                                  <  1:563387/140‑1 (MQ=255)                                                                                 ttgattTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGt                                                  <  2:1156186/140‑1 (MQ=255)                                                                                 ttgattTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGt                                                  <  2:1041779/140‑1 (MQ=255)                                                                                    attTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTCCGTAAAGAAAAAGGCGATGAGGATCGGAACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACg                                               <  1:930080/140‑1 (MQ=255)                                                                                       tAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTg                                            >  1:1107222/1‑140 (MQ=255)                                                                                          cgcAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTgaag                                         >  2:1369581/1‑140 (MQ=255)                                                                                          cgcAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTgaag                                         >  1:963128/1‑140 (MQ=255)                                                                                              aTTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTGAAGAACg                                     >  2:446363/1‑140 (MQ=255)                                                                                               ttttGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTGAAGAACgg                                    >  2:1380010/1‑140 (MQ=255)                                                                                                      ggcAGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTGAAGAACGGGCAGGGt                             <  1:1380010/140‑1 (MQ=255)                                                                                                       gcAGGGATCTACCGCCAGAGAGGTATTACGTAGAAAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTGAAGAACGGGCAGGGTg                            <  2:994036/140‑1 (MQ=255)                                                                                                       gaggggATCTCCCGCAAGAGAGGTATTACGAAGAAAAAAAGGCGATGAGGATCGGCACTAACAGGCAAAGAAAAAACCCGTGAACAATTGCCGCCGGGACCATTTCCCGCCCGCCAGTACGTTGAAGAACGGGCAGGGTg                            <  2:1076294/137‑1 (MQ=255)                                                                                                         aGGGATCTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTGAAGAACGGGCAGGGTGaa                          >  1:1076294/1‑140 (MQ=255)                                                                                                               cTACCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCGGGGACCATATCCAGCCCGCCAGTACGTTGAAGAACGGGCAGGGTGAAATCCAt                    <  1:792979/140‑1 (MQ=255)                                                                                                                 aCCGCCAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTGAAGAACGGGCAGGGTGAAATCCATTg                  >  1:816183/1‑140 (MQ=255)                                                                                                                     ccAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTGAAGAACGGGCAGGGTGAAATCCATTGAtgt              >  1:246349/1‑140 (MQ=255)                                                                                                                      cAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTGAAGAACGGGCAGGGTGAAATCCATTGAtg               >  1:712986/1‑138 (MQ=255)                                                                                                                      cAGAGAGGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTGAAGAACGGGCAGGGTGAAATCCATTGAtg               <  2:712986/138‑1 (MQ=255)                                                                                                                       agagagGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTGAAGAACGGGCAGGGTGAAATCCATTGATGTgg            >  2:1399994/1‑140 (MQ=255)                                                                                                                         ggagGTATTACGAAGAGAAAAAGGCGATGAGGATGGGCACTAACAGGCTAAGAAAAAACCCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTGAAGAACGGGCAGGGTGAAATCCATTGATGTGGCa          <  2:1338179/139‑1 (MQ=255)                                                                                                                         agagGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTGAAGAACGGGCAGGGTGAAATCCATTGATGTGGCa          <  1:353987/140‑1 (MQ=255)                                                                                                                          tagatATTACGAAGATAAAAAGGTGATGAGAATCGACACAAAAAGGCTAACAATAAAACCGTGAACAACTGCCGCCGGAACCATATCCAGCCCGCCAGTACGTTGAAGAACGGGCAGGGTGAAATCCATTGATGTGGCAc         <  2:1157756/136‑1 (MQ=255)                                                                                                                          gagGTATTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTGAAGAACGGGCAGGGTGAAATCCATTGATGTGGCAc         <  1:1369581/140‑1 (MQ=255)                                                                                                                               aTTACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTGAAGAACGGGCAGGGTGAAATCCATTGATGTGGCACCGCAt    >  2:272763/1‑140 (MQ=255)                                                                                                                                ttACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTGAAGAACGGGCAGGGTGAAATCCATTGATGTGGCACCGCATa   >  1:892882/1‑140 (MQ=255)                                                                                                                                ttACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTGAAGAACGGGCAGGGTGAAATCCATTGATGTGGCACCGCATa   >  2:1249560/1‑140 (MQ=255)                                                                                                                                 tACGTAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTGAAGAACGGGCAGGGTGAAATCCATTGATGTGGCACCGCATaa  <  1:490895/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        GTTATATTTAATATACATGTTTAAGGTTAAGACGCTTAACGCGGGGATAAAAGGGATTTTTCATGCAACTTTAAGGGAGATTGATTTAGCGCAATTTTGGCGGCAGGGATCTACCGCCAGAGAGGTATTACGCAGAGAAAAAGGCGATGAGGATCGGCACTAACAGGCTAAGAATAAAACCGTGAACAATTGCCGCCGGGACCATATCCAGCCCGCCAGTACGTTGAAGAACGGGCAGGGTGAAATCCATTGATGTGGCACCGCATAA  >  NC_000913/913831‑914098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑