Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 950,485 A→G L199P (CTT→CCT)  pflA ← pyruvate formate‑lyase activating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913950,4850AG100.0% 375.4 / NA 104L199P (CTT→CCT) pflApyruvate formate‑lyase activating enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (48/56);  total (48/56)

Only 100 of 104 total aligned reads displayed.

TACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACACCTTCACATTTTTGTTCGCCAGATATTT  >  NC_000913/950349‑950618
                                                                                                                                        |                                                                                                                                     
tACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCt                                                                                                                                    >  1:677221/1‑140 (MQ=255)
         aCCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTtct                                                                                                                           <  2:495861/140‑1 (MQ=255)
          ccGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTC‑TCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCa                                                                                                                         >  1:1224623/1‑140 (MQ=255)
             tACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCg                                                                                                                                  >  1:77440/1‑129 (MQ=255)
             tACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCg                                                                                                                                  <  2:77440/129‑1 (MQ=255)
             tACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAAc                                                                                                                       >  2:48662/1‑140 (MQ=255)
             tACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAAc                                                                                                                       <  2:878212/140‑1 (MQ=255)
             tACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAAc                                                                                                                       <  2:708919/140‑1 (MQ=255)
              aCTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACg                                                                                                                      <  2:101022/140‑1 (MQ=255)
              aCTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACg                                                                                                                      <  1:272879/140‑1 (MQ=255)
               cTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGt                                                                                                                     <  2:1248212/140‑1 (MQ=255)
                  cTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACg                                                                                                                      >  2:1087318/1‑136 (MQ=255)
                  cTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACg                                                                                                                      <  2:727504/136‑1 (MQ=255)
                  cTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACg                                                                                                                      >  1:727504/1‑136 (MQ=255)
                  cTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACg                                                                                                                      <  1:1087318/136‑1 (MQ=255)
                  cTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGt                                                                                                                     <  1:866150/137‑1 (MQ=255)
                  cTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGt                                                                                                                     >  2:866150/1‑137 (MQ=255)
                  cTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGc                                                                                                                  >  2:1183462/1‑140 (MQ=255)
                     aaGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCa                                                                                                               >  1:593569/1‑140 (MQ=255)
                          tGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGTTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCa                                                                                                          <  2:1276749/140‑1 (MQ=255)
                          tGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACg                                                                                                                      <  2:1208862/128‑1 (MQ=255)
                          tGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACg                                                                                                                      >  2:673310/1‑128 (MQ=255)
                          tGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACg                                                                                                                      <  1:673310/128‑1 (MQ=255)
                          tGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACg                                                                                                                      >  1:1208862/1‑128 (MQ=255)
                          tGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCa                                                                                                          <  2:1199920/140‑1 (MQ=255)
                          tGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCa                                                                                                          >  1:1061958/1‑140 (MQ=255)
                          tGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCa                                                                                                          <  2:1522748/140‑1 (MQ=255)
                            ccTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACg                                                                                                                      <  2:689065/126‑1 (MQ=255)
                            ccTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACg                                                                                                                      >  2:1014003/1‑126 (MQ=255)
                            ccTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACg                                                                                                                      >  1:689065/1‑126 (MQ=255)
                            ccTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACg                                                                                                                      <  1:1014003/126‑1 (MQ=255)
                            ccTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACg                                                                                                        >  1:937566/1‑140 (MQ=255)
                            ccTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACg                                                                                                        >  1:407263/1‑140 (MQ=255)
                              tttCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACggg                                                                                                      <  1:1054383/140‑1 (MQ=255)
                                   cgcgTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAAt                                                                                                 >  2:167285/1‑140 (MQ=255)
                                    gcgTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAAtt                                                                                                <  2:1061958/140‑1 (MQ=255)
                                      gTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGGAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCAAACCCCGGGTAAAttta                                                                                              >  2:55425/1‑138 (MQ=255)
                                          cATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGa                                                                                          >  1:768925/1‑140 (MQ=255)
                                              gTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCg                                                                                      <  2:1168182/140‑1 (MQ=255)
                                                   tttcttCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATgcg                                                                                 >  1:1478116/1‑140 (MQ=255)
                                                     tcttcGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCt                                                                               <  2:937566/140‑1 (MQ=255)
                                                        tcGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGaa                                                                            <  2:1224623/140‑1 (MQ=255)
                                                            tggtTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAatcgt                                                                        >  1:1547824/1‑140 (MQ=255)
                                                             ggtTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAatcgtc                                                                       <  1:48662/140‑1 (MQ=255)
                                                                  aaCACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAatcgtcatcgt                                                                  <  2:593569/140‑1 (MQ=255)
                                                                    cacCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAAtcgtcatcgtca                                                                >  2:749376/1‑140 (MQ=255)
                                                                      ccGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGa                                                              <  1:425349/140‑1 (MQ=255)
                                                                      ccGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGa                                                              >  2:1655350/1‑140 (MQ=255)
                                                                       cGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGAc                                                             <  2:677221/140‑1 (MQ=255)
                                                                       cGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGAc                                                             <  2:882460/140‑1 (MQ=255)
                                                                        gTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGa                                                              >  2:1556510/1‑138 (MQ=255)
                                                                        gTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGa                                                              <  1:1556510/138‑1 (MQ=255)
                                                                        gTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGa                                                              >  2:296984/1‑138 (MQ=255)
                                                                        gTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGa                                                              <  1:296984/138‑1 (MQ=255)
                                                                        gTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGAcc                                                            >  1:55764/1‑140 (MQ=255)
                                                                        gTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGAcc                                                            >  2:272567/1‑140 (MQ=255)
                                                                        gTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGAc                                                             >  2:367799/1‑139 (MQ=255)
                                                                        gTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGAc                                                             <  1:367799/139‑1 (MQ=255)
                                                                        gTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGAc                                                             >  1:1071252/1‑139 (MQ=255)
                                                                        gTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGAc                                                             <  2:1071252/139‑1 (MQ=255)
                                                                         tCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGAc                                                             >  1:963746/1‑138 (MQ=255)
                                                                         tCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGAc                                                             <  2:963746/138‑1 (MQ=255)
                                                                         tCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCa                                                           >  1:206074/1‑140 (MQ=255)
                                                                         tCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCa                                                           >  1:65295/1‑140 (MQ=255)
                                                                             gTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCt                                                       <  1:272567/140‑1 (MQ=255)
                                                                                  tACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGac                                                  <  2:1547824/140‑1 (MQ=255)
                                                                                   aCTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGAc                                                             >  2:380471/1‑128 (MQ=255)
                                                                                   aCTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGAc                                                             <  1:380471/128‑1 (MQ=255)
                                                                                   aCTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGaca                                                 <  2:1478116/140‑1 (MQ=255)
                                                                                   aCTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGaca                                                 <  1:1183462/140‑1 (MQ=255)
                                                                                       ttCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGAc                                                             >  2:289268/1‑124 (MQ=255)
                                                                                       ttCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGAc                                                             <  1:289268/124‑1 (MQ=255)
                                                                                       ttCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGAcaacaa                                             >  2:1283957/1‑140 (MQ=255)
                                                                                       ttCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGAcaacaa                                             <  1:978969/140‑1 (MQ=255)
                                                                                         cACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACg                                           >  1:1024353/1‑140 (MQ=255)
                                                                                            ccATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAg                                        <  2:206074/140‑1 (MQ=255)
                                                                                            ccATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAg                                        <  1:1655350/140‑1 (MQ=255)
                                                                                             cATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGGGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAgt                                       <  2:981190/140‑2 (MQ=255)
                                                                                               ttGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCgg                                     >  2:496620/1‑140 (MQ=255)
                                                                                                 gCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGAt                                   >  2:1346361/1‑140 (MQ=255)
                                                                                                 gCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGAt                                   >  2:768465/1‑140 (MQ=255)
                                                                                                    aCCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCca                                <  1:700680/140‑1 (MQ=255)
                                                                                                    aCCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCca                                <  1:989660/140‑1 (MQ=255)
                                                                                                      ccATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCcaca                              >  2:1281605/1‑140 (MQ=255)
                                                                                                       cATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCacac                             <  1:1283957/140‑1 (MQ=255)
                                                                                                         tttGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACACCt                           <  2:630942/140‑1 (MQ=255)
                                                                                                         tttGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACACCt                           >  1:630942/1‑140 (MQ=255)
                                                                                                          ttGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACACCtt                          >  2:415608/1‑140 (MQ=255)
                                                                                                          ttGTGTTTGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACACCtt                          <  1:768465/140‑1 (MQ=255)
                                                                                                               tttGCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACACCTTCACAt                     <  2:768925/140‑1 (MQ=255)
                                                                                                                  gCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACACCTTCACAtttt                  >  2:745770/1‑140 (MQ=255)
                                                                                                                  gCCCAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACACCTTCACAtttt                  >  2:1389164/1‑140 (MQ=255)
                                                                                                                    ccAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACACCTTCACATTTTTg                <  1:947815/140‑1 (MQ=255)
                                                                                                                    ccAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACACCTTCACATTTTTg                <  2:55764/140‑1 (MQ=255)
                                                                                                                     cAGCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACACCTTCACATTTTTGt               <  2:1024353/140‑1 (MQ=255)
                                                                                                                       gCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACACCTTCACATTTTTGTTc             <  2:1106806/140‑1 (MQ=255)
                                                                                                                       gCTCGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACACCTTCACATTTTTGTTc             >  1:585263/1‑140 (MQ=255)
                                                                                                                          cGTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACACCTTCACATTTTTGTTCGcc          <  1:749376/140‑1 (MQ=255)
                                                                                                                           gTGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACACCTTCACATTTTTGTTCGCCa         <  2:585263/140‑1 (MQ=255)
                                                                                                                            tGGTAGGGGAGAGGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGTCAACAACGTAGCGGATCCACACCTTCACATTTTTGTTCGCCAg        >  1:473487/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                     
TACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACACCTTCACATTTTTGTTCGCCAGATATTT  >  NC_000913/950349‑950618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑