Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	990,058	A→G	V100A (GTG→GCG)	pncB ←	nicotinate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	990,058	0	A	G	100.0%	324.4 / NA	91	V100A (GTG→GCG)	pncB	nicotinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (48/43);  total (48/43)

TGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGAACCTGTTCAC  >  NC_000913/989923‑990193                                                                                                                                        |                                                                                                                                        tGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGt                                                                                                                                     >  2:317007/1‑140 (MQ=255) tGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGt                                                                                                                                     >  1:1072121/1‑140 (MQ=255)  gcgcAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAACAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTg                                                                                                                                    >  2:912097/1‑140 (MQ=255)    ccaccGTCGGCCTGCGGTGAGGGATAGCGATGTACCATTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGAc                                                                                                                                  <  1:867010/136‑1 (MQ=255)        cGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGt                                                                                                                              >  1:1583042/1‑140 (MQ=255)        cGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGt                                                                                                                              >  1:1632788/1‑140 (MQ=255)        cGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGt                                                                                                                              >  1:10456/1‑140 (MQ=255)          tCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCATGCCACGGGCCGCTTAAACGAATATCCGGCTTGCCATTATCGTTGGACGCGGTGACTTGTTc                                                                                                                            >  1:333561/1‑140 (MQ=255)             gCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCgg                                                                                                                         >  1:310966/1‑140 (MQ=255)                  cGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGtt                                                                                                                      <  2:1567086/138‑1 (MQ=255)                  cGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGtt                                                                                                                      >  1:1567086/1‑138 (MQ=255)                  cGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTaa                                                                                                                    >  2:1267033/1‑140 (MQ=255)                        gcgatagcgatGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGa                                                                                                              >  1:431292/1‑140 (MQ=255)                               cgatGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTcgcg                                                                                                       >  2:664981/1‑140 (MQ=255)                                gatGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGt                                                                                                      <  1:1239023/140‑1 (MQ=255)                                gatGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGt                                                                                                      <  1:1622336/140‑1 (MQ=255)                                 atGTCCCTTTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTa                                                                                                     <  1:268013/140‑1 (MQ=255)                                 atGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTa                                                                                                     >  1:34136/1‑140 (MQ=255)                                 atGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTa                                                                                                     <  2:310966/140‑1 (MQ=255)                                 atGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTa                                                                                                     >  1:786482/1‑140 (MQ=255)                                  tGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCATGCCACGGGCCGCTTAAACGAATATCCGGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                                    <  2:333561/140‑1 (MQ=255)                                  tGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                                    <  1:664981/140‑1 (MQ=255)                                  tGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                                    <  2:1139084/140‑1 (MQ=255)                                  tGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                                    <  2:1186429/140‑1 (MQ=255)                                  tGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                                    <  2:34136/140‑1 (MQ=255)                                  tGTACCATTTCACGAAAACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                                    <  1:434428/140‑1 (MQ=255)                                    tACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAAcc                                                                                                  <  2:786482/140‑1 (MQ=255)                                    tACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAAcc                                                                                                  >  1:1141369/1‑140 (MQ=255)                                    tACCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAAcc                                                                                                  <  1:341082/140‑1 (MQ=255)                                     aCCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCa                                                                                                 >  2:261467/1‑140 (MQ=255)                                      ccATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTa                                                                                                     >  1:605740/1‑135 (MQ=255)                                      ccATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTa                                                                                                     <  2:605740/135‑1 (MQ=255)                                      ccATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAg                                                                                                >  1:1356240/1‑140 (MQ=255)                                      ccATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAg                                                                                                >  1:35877/1‑140 (MQ=255)                                            cACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGa                                                                                          >  2:543506/1‑140 (MQ=255)                                            cACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGa                                                                                          >  1:1066552/1‑140 (MQ=255)                                            cACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGa                                                                                          >  2:263960/1‑140 (MQ=255)                                              cTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAGTATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATa                                                                                        >  2:342120/1‑140 (MQ=255)                                              cTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                                    >  2:1234068/1‑128 (MQ=255)                                              cTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                                    <  1:1234068/128‑1 (MQ=255)                                              cTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATa                                                                                        >  1:350210/1‑140 (MQ=255)                                              cTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATa                                                                                        >  1:865105/1‑140 (MQ=255)                                                gATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                                                                                      <  1:1538098/140‑1 (MQ=255)                                                gATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                                                                                      <  1:317007/140‑1 (MQ=255)                                                gATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                                                                                      <  2:1072121/140‑1 (MQ=255)                                                 aTAACCGCCAGCAAAGGAACTTCCCGGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTc                                                                                     <  1:479857/140‑1 (MQ=255)                                                 aTAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                                                                                      >  2:316135/1‑139 (MQ=255)                                                 aTAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                                                                                      <  1:316135/139‑1 (MQ=255)                                                 aTAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTc                                                                                     <  1:1660866/140‑1 (MQ=255)                                                    aCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGc                                                                                  >  1:1043450/1‑140 (MQ=255)                                                      cGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                                <  2:865105/140‑1 (MQ=255)                                                      cGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                                <  1:1267033/140‑1 (MQ=255)                                                           gCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTaaag                                                                           >  1:283709/1‑140 (MQ=255)                                                                ggAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAgg                                                                      <  1:578603/140‑1 (MQ=255)                                                                ggAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAgg                                                                      <  2:1583042/140‑1 (MQ=255)                                                                   aCTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggcag                                                                   >  2:1437556/1‑140 (MQ=255)                                                                     ttCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAGTATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggcaggg                                                                 <  1:342120/140‑1 (MQ=255)                                                                      tCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggcagggc                                                                >  2:968627/1‑140 (MQ=255)                                                                       cccAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggcagggca                                                               <  1:543506/140‑1 (MQ=255)                                                                            agGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCC‑GGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGc                                                         <  1:1180084/140‑1 (MQ=255)                                                                             ggATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGc                                                         <  2:352024/140‑1 (MQ=255)                                                                              gATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGcc                                                        >  2:1166866/1‑140 (MQ=255)                                                                              gATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGcc                                                        <  2:350210/140‑1 (MQ=255)                                                                              gATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGCTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGcc                                                        >  2:244015/1‑140 (MQ=255)                                                                               aTGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGaa                                                            <  2:574729/135‑1 (MQ=255)                                                                               aTGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGaa                                                            >  1:574729/1‑135 (MQ=255)                                                                                tGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCAc                                                      <  1:263960/140‑1 (MQ=255)                                                                                 gACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGc                                                         >  1:629543/1‑136 (MQ=255)                                                                                 gACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGc                                                         <  2:629543/136‑1 (MQ=255)                                                                                 gACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACt                                                     >  1:915240/1‑140 (MQ=255)                                                                                    ttCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGat                                                  <  1:1166866/140‑1 (MQ=255)                                                                                    ttCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGCTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGat                                                  <  1:244015/140‑1 (MQ=255)                                                                                      cACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGatat                                                >  1:180681/1‑140 (MQ=255)                                                                                             cGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTcatcat                                         >  1:265856/1‑140 (MQ=255)                                                                                              gggCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCatcatc                                        >  2:628770/1‑140 (MQ=255)                                                                                              gggCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCatcatc                                        >  2:1264969/1‑140 (MQ=255)                                                                                                    cTTAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAg                                  >  2:727480/1‑140 (MQ=255)                                                                                                      tAAACGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgc                                <  1:1437556/140‑1 (MQ=255)                                                                                                        aaCGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgcgc                              <  2:265856/140‑1 (MQ=255)                                                                                                        aaCGAATATCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgcgc                              <  2:1043450/140‑1 (MQ=255)                                                                                                              tatCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGtgc                        >  1:309904/1‑140 (MQ=255)                                                                                                               atCCAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGtgct                       >  2:440628/1‑140 (MQ=255)                                                                                                                  cAGCTTGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa                    <  1:334933/140‑1 (MQ=255)                                                                                                                      ttGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgcgc                              >  2:1079530/1‑126 (MQ=255)                                                                                                                      ttGCCATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgcgc                              <  1:1079530/126‑1 (MQ=255)                                                                                                                         ccATTATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGCGCTGCATCGCCTg             >  1:1516229/1‑140 (MQ=255)                                                                                                                            ttATCGTTGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGAAc          <  1:1101830/140‑1 (MQ=255)                                                                                                                             tATCGTTGGACGCGTTGACTTGTTCCGGGATAGAGCCGAACTCGGGTAACCAGTTAAGCTACTCGTCCTTAACGGAAGATATGGCAAATAGCCACCGATATTCATCATCCTGCACGTGCAGGTACTAAAACGCCGGAAcc         >  2:1101830/1‑140 (MQ=255)                                                                                                                                  ttGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGAACCTGTTc    >  1:113102/1‑140 (MQ=255)                                                                                                                                  ttGGACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGAACCTGTTc    <  1:1025017/140‑1 (MQ=255)                                                                                                                                    ggACGCGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGAACCTGTTCAc  >  2:674596/1‑140 (MQ=255)                                                                                                                                    ggACGCGGTAACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGAACCTGTTCAc  >  2:1220577/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        TGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGAACCTGTTCAC  >  NC_000913/989923‑990193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑