Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,158,446	T→C	V193A (GTT→GCT)	ptsG →	glucose‑specific PTS enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,158,446	0	T	C	100.0%	297.0 / NA	83	V193A (GTT→GCT)	ptsG	glucose‑specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/43);  total (40/43)

GGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCG  >  NC_000913/1158313‑1158582                                                                                                                                      |                                                                                                                                         ggTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGtt                                                                                                                                    <  1:424140/140‑1 (MQ=255)  gTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGttt                                                                                                                                   <  2:671429/140‑1 (MQ=255)   tAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTg                                                                                                                                  <  1:96920/140‑1 (MQ=255)     aaCGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGc                                                                                                                                >  1:206726/1‑140 (MQ=255)             gtgCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGttt                                                                                                                                   <  1:1089677/129‑1 (MQ=255)             gtgCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGttt                                                                                                                                   >  2:1089677/1‑129 (MQ=255)                 cGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTc                                                                                                                    >  1:374671/1‑140 (MQ=255)                  gATCATTTCTGGCCTGGCTGCGATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTg                                                                                                                                  >  2:1182542/1‑125 (MQ=255)                  gATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTg                                                                                                                                  <  1:1182542/125‑1 (MQ=255)                    tCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATc                                                                                                                 <  1:448324/140‑1 (MQ=255)                       tttCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGaa                                                                                                              <  2:550144/140‑1 (MQ=255)                         tCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACg                                                                                                            <  2:1134532/140‑1 (MQ=255)                         tCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAAAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACg                                                                                                            <  1:6812/140‑1 (MQ=255)                           tggcctggcTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGtt                                                                                                          <  1:467180/140‑1 (MQ=255)                                tggcTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTg                                                                                                     >  1:893801/1‑140 (MQ=255)                                  gcTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTg                                                                                                     <  1:805120/138‑1 (MQ=255)                                  gcTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTg                                                                                                     >  2:805120/1‑138 (MQ=255)                                  gcTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGt                                                                                                   >  1:1349914/1‑140 (MQ=255)                                  gcTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGt                                                                                                   >  2:610850/1‑140 (MQ=255)                                  gcTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGt                                                                                                   >  1:590924/1‑140 (MQ=255)                                  gcTGCCATCTTTACAGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGt                                                                                                   >  2:755088/1‑140 (MQ=255)                                          cTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTg                                                                                           <  2:1349914/140‑1 (MQ=255)                                             tACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTc                                                                                        <  2:590924/140‑1 (MQ=255)                                                tGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGc                                                                                     <  1:990165/140‑1 (MQ=255)                                                  gCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGt                                                                                              >  1:1416115/1‑129 (MQ=255)                                                  gCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGt                                                                                              <  2:1416115/129‑1 (MQ=255)                                                  gCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGcac                                                                                   <  2:206726/140‑1 (MQ=255)                                                  gCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGcac                                                                                   <  1:318047/140‑1 (MQ=255)                                                   cGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGcacc                                                                                  <  1:1211354/140‑1 (MQ=255)                                                     ttGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGcaccac                                                                                >  1:602398/1‑140 (MQ=255)                                                     ttGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGcaccac                                                                                >  2:430593/1‑140 (MQ=255)                                                     ttGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGcaccac                                                                                >  1:1037056/1‑140 (MQ=255)                                                     ttGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGcaccac                                                                                >  2:503152/1‑140 (MQ=255)                                                     ttGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGcac                                                                                   >  1:599398/1‑137 (MQ=255)                                                     ttGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGcac                                                                                   <  2:599398/137‑1 (MQ=255)                                                       gtgCTGTCCTTCATTTGGCCGCCGTTTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACAt                                                                              >  1:1411710/1‑140 (MQ=255)                                                        tgCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATc                                                                             <  1:1168683/140‑1 (MQ=255)                                                               cTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACg                                                                      <  1:30029/140‑1 (MQ=255)                                                                  cATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTAc                                                                   >  2:1593724/1‑140 (MQ=255)                                                                  cATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTAc                                                                   >  2:309932/1‑140 (MQ=255)                                                                     ttGGCCGCCGTTTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCtt                                                                <  2:1411710/140‑1 (MQ=255)                                                                       ggCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTc                                                              <  2:1037056/140‑1 (MQ=255)                                                                       ggCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTc                                                              <  2:602398/140‑1 (MQ=255)                                                                       ggCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTc                                                              >  2:1191548/1‑140 (MQ=255)                                                                       ggCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTc                                                              >  2:803113/1‑140 (MQ=255)                                                                       ggCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTc                                                              >  2:90173/1‑140 (MQ=255)                                                                       ggCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTc                                                              >  1:491670/1‑140 (MQ=255)                                                                            ccgATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGAt                                                         >  1:738433/1‑140 (MQ=255)                                                                            ccgATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGAt                                                         >  2:874519/1‑140 (MQ=255)                                                                            ccgATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGAt                                                         <  1:430593/140‑1 (MQ=255)                                                                               aTTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCa                                                      <  2:893801/140‑1 (MQ=255)                                                                                 tGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCAATTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGa                                                    <  1:1170136/140‑1 (MQ=255)                                                                                    ttCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTg                                                 <  1:1593724/140‑1 (MQ=255)                                                                                    ttCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTg                                                 <  1:803113/140‑1 (MQ=255)                                                                                    ttCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTg                                                 <  2:775910/140‑1 (MQ=255)                                                                                      cTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGt                                               <  1:610850/140‑1 (MQ=255)                                                                                             ccAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATaca                                        >  1:893051/1‑140 (MQ=255)                                                                                              cAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGATTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCa                                                      >  2:143932/1‑125 (MQ=255)                                                                                              cAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGATTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCa                                                      <  1:143932/125‑1 (MQ=255)                                                                                                 aCCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCa                                                      <  1:54968/122‑1 (MQ=255)                                                                                                 aCCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCa                                                      >  2:54968/1‑122 (MQ=255)                                                                                                 aCCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCaa                                    >  1:1263133/1‑140 (MQ=255)                                                                                                    ttCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACgc                                 <  2:1263133/140‑1 (MQ=255)                                                                                                      ctctcAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACgcag                               <  2:1415829/140‑1 (MQ=255)                                                                                                      ctctcAGTGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTCACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACgcag                               >  1:168768/1‑140 (MQ=255)                                                                                                             tGGGCTGCTTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGcaggtca                        >  1:1424380/1‑140 (MQ=255)                                                                                                                gctgctTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGcaggtcaggt                     >  2:291780/1‑140 (MQ=255)                                                                                                                gctgctTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGcaggtcaggt                     >  1:1025312/1‑140 (MQ=255)                                                                                                                gctgctTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGcaggtcaggt                     >  1:1476446/1‑140 (MQ=255)                                                                                                                gctgctTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGcaggtcaggt                     >  2:532467/1‑140 (MQ=255)                                                                                                                 ctgctTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGtt                    >  1:240605/1‑140 (MQ=255)                                                                                                                 ctgctTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGtt                    >  2:847088/1‑140 (MQ=255)                                                                                                                 ctgctTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGtt                    >  2:1297387/1‑140 (MQ=255)                                                                                                                  tgctTACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGttt                   <  1:755088/140‑1 (MQ=255)                                                                                                                      tACCAGAACCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCa               <  2:491670/140‑1 (MQ=255)                                                                                                                           gAACCCGGTAGCTGCGTTTGGCATTTGCGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCCCGGCg          <  2:1586519/140‑1 (MQ=255)                                                                                                                             aCCCGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCATCGCAGCAGGTCAGGTTTTCCACGGCGAc        <  2:374671/140‑1 (MQ=255)                                                                                                                              cccGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACa       >  2:1163922/1‑140 (MQ=255)                                                                                                                                cGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACAtt     <  2:949916/140‑1 (MQ=255)                                                                                                                                cGGTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACAtt     <  1:219204/140‑1 (MQ=255)                                                                                                                                  gTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCa               >  2:1033934/1‑128 (MQ=255)                                                                                                                                  gTAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCa               <  1:1033934/128‑1 (MQ=255)                                                                                                                                   tAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCcg  <  2:355010/140‑1 (MQ=255)                                                                                                                                   tAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCcg  <  1:309932/140‑1 (MQ=255)                                                                                                                                   tAGCTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCcg  <  1:195941/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         GGTAAACGCTTTGTGCCGATCATTTCTGGCCTGGCTGCCATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCG  >  NC_000913/1158313‑1158582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑