Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,262,390	A→C	V163G (GTG→GGG)	ispE ←	4‑(cytidine 5'‑diphospho)‑2‑C‑methyl‑D‑erythritol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,262,390	0	A	C	100.0%	208.1 / NA	59	V163G (GTG→GGG)	ispE	4‑(cytidine 5'‑diphospho)‑2‑C‑methyl‑D‑erythritol kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (36/23);  total (36/23)

CTGAATTCACATTTTAGCAACGTTTCTATTGACCTTTTTGGCGTATTGCGCGGGAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCACCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATT  >  NC_000913/1262255‑1262523                                                                                                                                        |                                                                                                                                      cTGAATTCACATTTTAGCAACGTTTCTATTGACCTTTTTGGCGTATTGCGCGGGAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCgg                                                                                                                                   >  1:87690/1‑140 (MQ=255)      ttCACATTTTAGCAACGTTTCTATTGACCTTTTTGGCGTATTGCGCGGGAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTa                                                                                                                              >  2:719323/1‑140 (MQ=255)                  cAACGTTTCTATTGACCTTTTTGGCGTATTGCGCGGGAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCaa                                                                                                                  <  1:719323/140‑1 (MQ=255)                         tCTATTGACCTTTTTGGCGTATTGCGCGGGAGTTCAGGATCTTTAATAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTc                                                                                                           >  2:804264/1‑140 (MQ=255)                         tCTATTGACCTTTTTGGCGTATTGCGCGGGAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTc                                                                                                           >  1:179023/1‑140 (MQ=255)                          cTATTGACCTTTTTGGCGTATTGCGCGGGAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCg                                                                                                          >  1:1456633/1‑140 (MQ=255)                            aTTGACCTTTTTGGCGTATTGCGCGGGAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGc                                                                                                        <  2:897401/140‑1 (MQ=255)                               gACCTTTTTGGCGTATTGCGCGGGAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCaaa                                                                                                     >  2:382098/1‑140 (MQ=255)                                aCCTTTTTGGCGTATTGCGCGGGAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAAc                                                                                                    >  1:789475/1‑140 (MQ=255)                                aCCTTTTTGGCGTATTGCGCGGGAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAAc                                                                                                    >  1:392456/1‑140 (MQ=255)                                       tGGCGTATTGCGCGGGAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCAt                                                                                             >  1:355817/1‑140 (MQ=255)                                          cGTATTGCGCGGGAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGcc                                                                                          <  2:355817/140‑1 (MQ=255)                                            tATTGCGCGGGAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGcccc                                                                                        <  2:1456633/140‑1 (MQ=255)                                              ttGCGCGGGAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCg                                                                                      >  2:25106/1‑140 (MQ=255)                                               tGCGCGGGAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGa                                                                                     <  1:1255631/140‑1 (MQ=255)                                                 cgcgGGAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAAc                                                                                   >  1:237155/1‑140 (MQ=255)                                                  gcgGGAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACa                                                                                  <  1:25106/140‑1 (MQ=255)                                                    gggAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACaaa                                                                                <  1:382098/140‑1 (MQ=255)                                                    gggAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACaaa                                                                                <  2:789475/140‑1 (MQ=255)                                                      gAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGa                                                                              >  2:675138/1‑140 (MQ=255)                                                            aGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGaa                                                                        <  2:179023/140‑1 (MQ=255)                                                                   ttAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTgc                                                                 >  1:242821/1‑140 (MQ=255)                                                                            aCCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGt                                                        <  2:392456/140‑1 (MQ=255)                                                                            aCCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGt                                                        >  1:908900/1‑140 (MQ=255)                                                                            aCCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGt                                                        <  1:1177148/140‑1 (MQ=255)                                                                                 aGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGcc                                                   <  1:675138/140‑1 (MQ=255)                                                                                              acacCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAg                                      <  2:242821/140‑1 (MQ=255)                                                                                                 ccAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTc                                   >  1:45623/1‑140 (MQ=255)                                                                                                    gggTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATc                                >  1:247904/1‑140 (MQ=255)                                                                                                     ggTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTGAGCCCCATTTCCGCCAGCTCATcc                               >  2:1664413/1‑140 (MQ=255)                                                                                                     ggTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAg                                      <  1:1576059/133‑1 (MQ=255)                                                                                                     ggTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAg                                      >  2:1576059/1‑133 (MQ=255)                                                                                                     ggTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATcc                               >  1:746982/1‑140 (MQ=255)                                                                                                     ggTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATcc                               >  1:503394/1‑140 (MQ=255)                                                                                                     ggTGCGCCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATcc                               >  2:1467010/1‑140 (MQ=255)                                                                                                         cgcCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGc                           >  2:387320/1‑140 (MQ=255)                                                                                                          gcCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCt                          >  1:235235/1‑140 (MQ=255)                                                                                                          gcCACCAGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCt                          >  1:280795/1‑140 (MQ=255)                                                                                                               caGATACCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGcc                     >  1:114812/1‑140 (MQ=255)                                                                                                                    aCCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATc                                >  2:597737/1‑124 (MQ=255)                                                                                                                    aCCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATc                                <  1:597737/124‑1 (MQ=255)                                                                                                                    aCCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGcc                     <  1:381414/135‑1 (MQ=255)                                                                                                                    aCCACTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGcc                     >  2:381414/1‑135 (MQ=255)                                                                                                                       aCTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGc             <  1:387320/140‑1 (MQ=255)                                                                                                                        cTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGcc            <  2:280795/140‑1 (MQ=255)                                                                                                                        cTTCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGcc            >  1:900669/1‑140 (MQ=255)                                                                                                                         ttCTCTGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCa           >  2:793414/1‑140 (MQ=255)                                                                                                                           ctctGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCaga         >  1:412575/1‑140 (MQ=255)                                                                                                                             ctGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAgaga       >  2:1358825/1‑140 (MQ=255)                                                                                                                              tGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGc                  >  1:841814/1‑128 (MQ=255)                                                                                                                              tGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGc                  <  2:841814/128‑1 (MQ=255)                                                                                                                              tGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGc             <  1:591196/133‑1 (MQ=255)                                                                                                                              tGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGc             >  2:591196/1‑133 (MQ=255)                                                                                                                              tGGCGGATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAgat      >  1:1295859/1‑140 (MQ=255)                                                                                                                                  ggATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGAtt  <  1:793414/140‑1 (MQ=255)                                                                                                                                   gATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGc             >  2:732657/1‑128 (MQ=255)                                                                                                                                   gATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGc             <  1:732657/128‑1 (MQ=255)                                                                                                                                   gATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAgatgat   <  2:640760/138‑1 (MQ=255)                                                                                                                                   gATCCCCCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAgatgat   >  1:640760/1‑138 (MQ=255)                                                                                                                                        |                                                                                                                                      CTGAATTCACATTTTAGCAACGTTTCTATTGACCTTTTTGGCGTATTGCGCGGGAGTTCAGGATCTTTAAAAATCACCGGAGTCGGAATACTTACACCAGGGTGCGCCACCAGATACCACTTCTCTGGCGGATCCACCGGCGTTAGTATTTCACCAACGCCTTCGGCAAACGCGGCATGCCCCCGAACAAAGACAGGAACATCTGCGCCCAGCGTCAGCCCCATTTCCGCCAGCTCATCCATGCTTAGCCCGCATTGCCAGAGATGATT  >  NC_000913/1262255‑1262523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑