Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,379,930	T→C	L683L (TTA→CTA)	ycjT →	kojibiose phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,379,930	0	T	C	100.0%	320.7 / NA	90	L683L (TTA→CTA)	ycjT	kojibiose phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (49/41);  total (49/41)

CGATCTTGGTGCTGATCCGCATAGTTGTGATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGTTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGCGTTTGAACGGTCAGCTTATAAC  >  NC_000913/1379797‑1380066                                                                                                                                      |                                                                                                                                         cGATCTTGGTGCTGATCCGCATAGTTGTGATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTAcc                                                                                                                                      <  2:1611790/138‑1 (MQ=255) cGATCTTGGTGCTGATCCGCATAGTTGTGATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTAcc                                                                                                                                      >  1:1611790/1‑138 (MQ=255)   aTCTTGGTGCTGATCCGCATAGTTGTGATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAg                                                                                                                                  >  2:660024/1‑140 (MQ=255)    tCTTGGTGCTGATCCGCATAGTTGTGATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGc                                                                                                                                 <  2:1215035/140‑1 (MQ=255)       tGGTGCTGATCCGCATAGTTGTGATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGt                                                                                                                              >  2:279176/1‑140 (MQ=255)         gTGCTGATCCGCATAGTTGTGATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTgg                                                                                                                            <  1:569810/140‑1 (MQ=255)          tGCTGATCCGCATAGTTGTGATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGc                                                                                                                           <  1:1440596/140‑1 (MQ=255)            cTGATCCGCATAGTTGTGATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCaa                                                                                                                         >  2:220683/1‑140 (MQ=255)              gATCCGCATAGTTGTGATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACa                                                                                                                       >  1:941514/1‑140 (MQ=255)               aTCCGCATAGTTGTGATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAg                                                                                                                      <  1:1009625/140‑1 (MQ=255)                    cATAGTTGTGATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTc                                                                                                                 >  1:80663/1‑140 (MQ=255)                      tAGTTGTGATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCtt                                                                                                               <  1:220683/140‑1 (MQ=255)                      tAGTTGTGATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCtt                                                                                                               <  1:1616027/140‑1 (MQ=255)                         ttGTGATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTcc                                                                                                            >  2:1110977/1‑140 (MQ=255)                          tgtgATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTccc                                                                                                           <  1:1110977/140‑1 (MQ=255)                           gtgATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCct                                                                                                          >  1:371850/1‑140 (MQ=255)                           gtgATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCct                                                                                                          <  1:257914/140‑1 (MQ=255)                           gtgATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCct                                                                                                          >  1:1507222/1‑140 (MQ=255)                            tgatgaTGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCctc                                                                                                         <  2:371850/140‑1 (MQ=255)                                 atgGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTc                                                                                                    >  2:1633944/1‑140 (MQ=255)                                  tgGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCt                                                                                                   >  2:494132/1‑140 (MQ=255)                                   ggTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCct                                                                                                          >  2:522839/1‑132 (MQ=255)                                   ggTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCct                                                                                                          <  1:522839/132‑1 (MQ=255)                                   ggTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGt                                                                                                      <  2:1157697/136‑1 (MQ=255)                                   ggTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGt                                                                                                      >  1:1157697/1‑136 (MQ=255)                                   ggTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTg                                                                                                  >  1:944772/1‑140 (MQ=255)                                      aTCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTccc                                                                                                           >  1:161372/1‑128 (MQ=255)                                      aTCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTccc                                                                                                           <  2:161372/128‑1 (MQ=255)                                      aTCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCa                                                                                               >  2:270948/1‑140 (MQ=255)                                       tCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAg                                                                                              >  2:9287/1‑140 (MQ=255)                                         cATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAggg                                                                                            <  2:1407963/140‑1 (MQ=255)                                           tgctgcCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCt                                                                                          >  2:1379893/1‑140 (MQ=255)                                            gctgcCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTg                                                                                         <  2:944772/140‑1 (MQ=255)                                            gctgcCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTg                                                                                         >  2:707856/1‑140 (MQ=255)                                            gctgcCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTg                                                                                         <  1:1501564/140‑1 (MQ=255)                                            gctgcCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTg                                                                                         >  2:1501564/1‑140 (MQ=255)                                            gctgcCGCAACTGGCGCTATCTGGCTGGGGGCGACTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTg                                                                                         >  1:76583/1‑140 (MQ=255)                                              tgcCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAggg                                                                                            >  1:417505/1‑135 (MQ=255)                                              tgcCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAggg                                                                                            <  2:417505/135‑1 (MQ=255)                                              tgcCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCg                                                                                       <  1:1430717/140‑1 (MQ=255)                                                 cGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTgg                                                                                                 <  2:1215699/127‑1 (MQ=255)                                                 cGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTgg                                                                                                 >  1:1215699/1‑127 (MQ=255)                                                 cGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTg                                                                                         <  2:1530892/135‑1 (MQ=255)                                                 cGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTg                                                                                         >  1:1648381/1‑135 (MQ=255)                                                 cGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTg                                                                                         <  2:1648381/135‑1 (MQ=255)                                                 cGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTg                                                                                         >  1:1530892/1‑135 (MQ=255)                                                  gCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAAtt                                                                                   <  1:1146985/140‑1 (MQ=255)                                                        ggCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGt                                                                             <  1:1633944/140‑1 (MQ=255)                                                        ggCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGt                                                                             <  1:270948/140‑1 (MQ=255)                                                               tCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCtt                                                                      >  2:224767/1‑140 (MQ=255)                                                                   gctggGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGAcg                                                                  >  2:611296/1‑140 (MQ=255)                                                                    ctggGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGAcgc                                                                 <  1:611296/140‑1 (MQ=255)                                                                     tggGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGAcgcg                                                                <  1:494132/140‑1 (MQ=255)                                                                       ggggCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCa                                                              <  1:279176/140‑1 (MQ=255)                                                                         ggCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGt                                                                             <  2:273808/123‑1 (MQ=255)                                                                         ggCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGt                                                                             >  1:273808/1‑123 (MQ=255)                                                                         ggCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCa                                                              <  2:73283/138‑1 (MQ=255)                                                                         ggCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCa                                                              >  1:73283/1‑138 (MQ=255)                                                                         ggCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGc                                                            >  2:970799/1‑140 (MQ=255)                                                                             aTTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTAt                                                        >  1:1253401/1‑140 (MQ=255)                                                                                        tGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCa                                                              >  2:1255821/1‑123 (MQ=255)                                                                                        tGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCa                                                              <  1:1255821/123‑1 (MQ=255)                                                                                        tGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGaa                                             >  2:497971/1‑140 (MQ=255)                                                                                             gggTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCt                                        <  1:970799/140‑1 (MQ=255)                                                                                                 gAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTgcgc                                    <  2:941514/140‑1 (MQ=255)                                                                                                  aGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGcc                                   >  1:626650/1‑140 (MQ=255)                                                                                                    cgtgcgtgACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCg                                 >  2:1304702/1‑140 (MQ=255)                                                                                                        cgtgACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTc                             >  1:1438115/1‑140 (MQ=255)                                                                                                        cgtgACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTc                             >  1:579374/1‑140 (MQ=255)                                                                                                         gtgACGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCa                            <  1:753423/140‑1 (MQ=255)                                                                                                            aCGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTg                         >  2:408638/1‑140 (MQ=255)                                                                                                             cGGTGAATTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGc                        >  1:162342/1‑140 (MQ=255)                                                                                                                  aaTTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCa                            <  1:354562/131‑1 (MQ=255)                                                                                                                  aaTTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCa                            >  2:354562/1‑131 (MQ=255)                                                                                                                   aTTGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGCGTTTGa                  >  2:1414201/1‑140 (MQ=255)                                                                                                                    ttGCATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGCGTTTGaa                 <  1:408638/140‑1 (MQ=255)                                                                                                                       cATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGCGTTTGAACgg              >  1:1112704/1‑140 (MQ=255)                                                                                                                       cATCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGCGTTTGAACgg              >  2:590317/1‑140 (MQ=255)                                                                                                                        aTCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTTACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGCGTTTGAACGGt             <  1:1314487/140‑1 (MQ=255)                                                                                                                        aTCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGCGTTTGAACGGt             <  2:626650/140‑1 (MQ=255)                                                                                                                        aTCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGCGTTTGAACGGt             <  1:224767/140‑1 (MQ=255)                                                                                                                        aTCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGCGTTTGAACGGt             <  2:579374/140‑1 (MQ=255)                                                                                                                        aTCTCAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGCGTTTGAACGGt             <  2:1592478/140‑1 (MQ=255)                                                                                                                         tctcAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGCGTTTGAACGGTc            >  1:1361838/1‑140 (MQ=255)                                                                                                                         tctcAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGCGTTTGAACGGTc            >  1:1320556/1‑140 (MQ=255)                                                                                                                          ctcAATCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGCGTTTGAACGGTCa           >  1:1049675/1‑140 (MQ=255)                                                                                                                            cAATCCGGCGCTACCTGAGCAGTGTCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGCGTTTGAACTGTCAGc         >  1:511753/1‑140 (MQ=255)                                                                                                                               tCCGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGCGTTTGAACGGTCAGCTta      >  1:1370559/1‑140 (MQ=255)                                                                                                                                ccGGCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGCGTTTGAACGGTCAGCTtat     <  2:162342/140‑1 (MQ=255)                                                                                                                                  ggCGCTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGCGTTTGAACGGTCAGCTTATaa   >  2:1288115/1‑140 (MQ=255)                                                                                                                                   gcgcTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGCGTTTGAACGGTCAGCTTATAAc  <  2:1370559/140‑1 (MQ=255)                                                                                                                                   gcgcTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGCGTTTGAACGGTCAGCTTATAAc  <  1:707856/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         CGATCTTGGTGCTGATCCGCATAGTTGTGATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTTTGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGTTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTCTGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTGCGTTTGAACGGTCAGCTTATAAC  >  NC_000913/1379797‑1380066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑