Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 133,045 C→T G477G (GGC→GGT)  acnB → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913133,0450CT98.8% 279.0 / ‑5.9 85G477G (GGC→GGT) acnBhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (42/42);  total (42/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGCGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGCAACTGGCG  >  NC_000913/132911‑133181
                                                                                                                                      |                                                                                                                                        
ttCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCAt                                                                                                                                     >  1:819403/1‑140 (MQ=255)
  ctcGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTc                                                                                                                                   >  1:1016597/1‑140 (MQ=255)
  ctcGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTc                                                                                                                                   >  2:1401987/1‑140 (MQ=255)
  ctcGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTc                                                                                                                                   >  2:1294935/1‑140 (MQ=255)
  ctcGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTc                                                                                                                                   >  2:1254973/1‑140 (MQ=255)
  ctcGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTc                                                                                                                                   >  1:848807/1‑140 (MQ=255)
  ctcGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTc                                                                                                                                   >  2:820293/1‑140 (MQ=255)
   tcGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCa                                                                                                                                  >  2:1445981/1‑140 (MQ=255)
     ggCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACt                                                                                                                                <  2:200519/140‑1 (MQ=255)
     ggCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACt                                                                                                                                <  2:388513/140‑1 (MQ=255)
       cTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTcc                                                                                                                              <  2:1013898/140‑1 (MQ=255)
            cTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCt                                                                                                                         <  1:833292/140‑1 (MQ=255)
            cTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCt                                                                                                                         <  1:1584889/140‑1 (MQ=255)
               gTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGaa                                                                                                                      <  2:1297582/140‑1 (MQ=255)
               gTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGaa                                                                                                                      <  2:291002/140‑1 (MQ=255)
                    gCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTa                                                                                                                 >  2:947860/1‑140 (MQ=255)
                      aGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTAtg                                                                                                               <  2:669163/140‑1 (MQ=255)
                      aGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTAtg                                                                                                               >  1:1512190/1‑140 (MQ=255)
                         cTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTAtgctg                                                                                                            >  2:500648/1‑140 (MQ=255)
                         cTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTAtgctg                                                                                                            <  1:820293/140‑1 (MQ=255)
                         cTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGCGTCATTCACTCCTGGCTGAACCGTAtgctg                                                                                                            <  2:265704/140‑1 (MQ=255)
                          tttCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTAtgctgc                                                                                                           >  2:665406/1‑140 (MQ=255)
                           ttCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTAtgctgct                                                                                                          <  2:1016597/140‑1 (MQ=255)
                            tCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTAtgctgctg                                                                                                         >  2:1311958/1‑140 (MQ=255)
                            tCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTAtgctgctg                                                                                                         <  1:1311958/140‑1 (MQ=255)
                             cTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTAtgctgctgc                                                                                                        >  2:342370/1‑140 (MQ=255)
                                ccACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCgg                                                                                                     >  2:297963/1‑140 (MQ=255)
                                  acacCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGAt                                                                                                   <  1:578799/140‑1 (MQ=255)
                                  acacCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGAt                                                                                                   <  1:665406/140‑1 (MQ=255)
                                  acacCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGAt                                                                                                   >  2:1036989/1‑140 (MQ=255)
                                      cgcgGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGAt                                                                                                   >  1:272596/1‑136 (MQ=255)
                                      cgcgGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGAt                                                                                                   <  2:272596/136‑1 (MQ=255)
                                      cgcgGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCg                                                                                               >  1:1371818/1‑140 (MQ=255)
                                       gcggcgTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGt                                                                                              >  2:421004/1‑140 (MQ=255)
                                        cggcgTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGa                                                                                                    <  2:339954/133‑1 (MQ=255)
                                        cggcgTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGa                                                                                                    >  1:339954/1‑133 (MQ=255)
                                        cggcgTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTc                                                                                             <  2:1371818/140‑1 (MQ=255)
                                        cggcgTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTc                                                                                             >  1:1581179/1‑140 (MQ=255)
                                            gTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTa                                                                                         <  1:1195948/140‑1 (MQ=255)
                                             tATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTAc                                                                                        <  1:1036989/140‑1 (MQ=255)
                                               tCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCg                                                                                      <  1:500648/140‑1 (MQ=255)
                                               tCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCg                                                                                      <  1:1445981/140‑1 (MQ=255)
                                                ccGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCgg                                                                                     >  2:1092109/1‑140 (MQ=255)
                                                     gCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGgtggtg                                                                                <  1:947860/140‑1 (MQ=255)
                                                     gCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGgtggtg                                                                                <  2:848807/140‑1 (MQ=255)
                                                      ccAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGa                                                                               <  1:1326139/140‑1 (MQ=255)
                                                            gACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACAGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCa                                                                         <  2:1512190/140‑1 (MQ=255)
                                                                tGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATAcc                                                                     <  2:1581179/140‑1 (MQ=255)
                                                                       gCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTcc                                                              >  2:976295/1‑140 (MQ=255)
                                                                       gCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTcc                                                              >  2:302002/1‑140 (MQ=255)
                                                                        caccacACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTccc                                                             <  2:819403/140‑1 (MQ=255)
                                                                         accacACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCg                                                            <  2:1299519/140‑1 (MQ=255)
                                                                          ccacACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGa                                                           <  1:421004/140‑1 (MQ=255)
                                                                            acacGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATc                                                         >  2:133416/1‑140 (MQ=255)
                                                                             cacGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCg                                                        >  2:10202/1‑140 (MQ=255)
                                                                             cacGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCg                                                        <  1:976295/140‑1 (MQ=255)
                                                                             cacGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCg                                                        >  2:925501/1‑140 (MQ=255)
                                                                                    ccGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTAtctc                                                 >  2:309538/1‑140 (MQ=255)
                                                                                     cGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATctct                                                >  1:1230563/1‑140 (MQ=255)
                                                                                        aCTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTc                                             <  2:991263/140‑1 (MQ=255)
                                                                                                aTGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCggg                                     <  2:1230563/140‑1 (MQ=255)
                                                                                                 tGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGt                                    <  1:1092109/140‑1 (MQ=255)
                                                                                                  gAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGtt                                   >  1:879664/1‑140 (MQ=255)
                                                                                                   aaCCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTtc                                  <  1:925501/140‑1 (MQ=255)
                                                                                                      cGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTtctgg                               >  1:1625568/1‑140 (MQ=255)
                                                                                                       gTGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTtctggt                              >  2:405598/1‑140 (MQ=255)
                                                                                                        tGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTtctggtc                             >  1:1644200/1‑140 (MQ=255)
                                                                                                        tGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTtctggtc                             >  1:1081895/1‑140 (MQ=255)
                                                                                                        tGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTtctggtc                             >  2:603568/1‑140 (MQ=255)
                                                                                                        tGGCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTtctggtc                             >  1:232740/1‑140 (MQ=255)
                                                                                                         ggCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCcggc                                        >  2:1645344/1‑128 (MQ=255)
                                                                                                         ggCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCcggc                                        <  1:1645344/128‑1 (MQ=255)
                                                                                                         ggCGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTtctggtct                            >  1:1369586/1‑140 (MQ=255)
                                                                                                           cGGTGTGTCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTtctggtctgg                          <  2:1081895/140‑1 (MQ=255)
                                                                                                             gtgtgtAGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCtggtg                        >  2:773506/1‑140 (MQ=255)
                                                                                                                  tCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGtt                   <  2:661039/140‑1 (MQ=255)
                                                                                                                  tCGCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGGCTGGTGGCggt                   >  1:1436326/1‑138 (MQ=255)
                                                                                                                    gCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTtg                 <  1:1254973/140‑1 (MQ=255)
                                                                                                                    gCTGCGTCCGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTtg                 <  1:10202/140‑1 (MQ=255)
                                                                                                                     cTGCGTCCGGGTGACGGTGTAATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTtgc                >  2:851122/1‑140 (MQ=255)
                                                                                                                            cGGGTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGCa         >  1:788149/1‑140 (MQ=255)
                                                                                                                              ggTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGCAAc       <  1:309538/140‑1 (MQ=255)
                                                                                                                              ggTGACGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGCAAc       <  2:1436326/140‑1 (MQ=255)
                                                                                                                              ggTGACGGTGTAATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGCAAc       <  1:851122/140‑1 (MQ=255)
                                                                                                                                   cGGTGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGCAACTGGCg  <  1:405598/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                        
TTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGAACCGTGGCGGTGTGTCGCTGCGTCCGGGTGACGGCGTCATTCACTCCTGGCTGAACCGTATGCTGCTGCCGGATACCGTCGGTACCGGTGGTGACTCCCATACCCGTTTCCCGATCGGTATCTCTTTCCCGGCGGGTTCTGGTCTGGTGGCGTTTGCTGCCGCAACTGGCG  >  NC_000913/132911‑133181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑