Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,684,414	G→A	P52P (CCG→CCA)	tus →	DNA replication terminus site‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,684,414	0	G	A	100.0%	353.3 / NA	99	P52P (CCG→CCA)	tus	DNA replication terminus site‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (48/51);  total (48/51)

GACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCGCTGTCCGTCTGCCTGGCGTG  >  NC_000913/1684280‑1684549                                                                                                                                       |                                                                                                                                        gACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTaa                                                                                                                                    <  1:542991/140‑1 (MQ=255) gACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTaa                                                                                                                                    <  1:79630/140‑1 (MQ=255)  aCCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAAt                                                                                                                                   <  2:613271/140‑1 (MQ=255)   ccGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATc                                                                                                                                  >  1:1345502/1‑140 (MQ=255)    cGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCg                                                                                                                                 >  2:522465/1‑140 (MQ=255)      aCTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTa                                                                                                                               >  1:974456/1‑140 (MQ=255)      aCTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTa                                                                                                                               >  2:1544906/1‑140 (MQ=255)      aCTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTa                                                                                                                               >  1:566414/1‑140 (MQ=255)      aCTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTa                                                                                                                               >  1:1510149/1‑140 (MQ=255)      aCTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTa                                                                                                                               >  1:1475066/1‑140 (MQ=255)      aCTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTa                                                                                                                               >  2:666095/1‑140 (MQ=255)      aCTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTa                                                                                                                               >  2:727485/1‑140 (MQ=255)       cTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTAt                                                                                                                              <  2:51895/140‑1 (MQ=255)         cAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTg                                                                                                                            <  2:1475066/140‑1 (MQ=255)         cAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTg                                                                                                                            <  1:522465/140‑1 (MQ=255)         cAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTg                                                                                                                            <  1:98938/140‑1 (MQ=255)            cacTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAgg                                                                                                                         <  2:423688/140‑1 (MQ=255)            cacTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAgg                                                                                                                         <  2:243031/140‑1 (MQ=255)                 ccTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTaaaa                                                                                                                    >  2:386226/1‑140 (MQ=255)                 ccTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTaaaa                                                                                                                    >  2:29687/1‑140 (MQ=255)                 ccTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTaaaa                                                                                                                    >  1:870570/1‑140 (MQ=255)                 ccTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTaaaa                                                                                                                    >  1:1049056/1‑140 (MQ=255)                 ccTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTaaaa                                                                                                                    >  1:309845/1‑140 (MQ=255)                  cTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAaac                                                                                                                   <  1:578504/140‑1 (MQ=255)                  cTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAaac                                                                                                                   <  1:1511598/140‑1 (MQ=255)                    ttCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAaacaa                                                                                                                 <  1:727485/140‑1 (MQ=255)                      cGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAacaaca                                                                                                               >  2:651869/1‑140 (MQ=255)                       gCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACAt                                                                                                              <  1:29687/140‑1 (MQ=255)                            aTGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCgg                                                                                                         <  2:309845/140‑1 (MQ=255)                                    agagCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAacgacg                                                                                                 <  2:566414/140‑1 (MQ=255)                                      agCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGAcgcg                                                                                               <  2:155015/140‑1 (MQ=255)                                       gCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACgcgc                                                                                              <  1:666095/140‑1 (MQ=255)                                        cTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCa                                                                                             >  2:267551/1‑140 (MQ=255)                                          ggCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGt                                                                                           >  1:54454/1‑140 (MQ=255)                                          ggCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGt                                                                                           >  1:974576/1‑140 (MQ=255)                                          ggCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGt                                                                                           >  1:1086174/1‑140 (MQ=255)                                          ggCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGt                                                                                           >  2:700505/1‑140 (MQ=255)                                            cTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAGGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCg                                                                                         >  2:154240/1‑140 (MQ=255)                                                     ccGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTg                                                                                >  2:1140307/1‑140 (MQ=255)                                                     ccGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTg                                                                                >  1:237710/1‑140 (MQ=255)                                                       gCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCg                                                                              <  2:653993/140‑1 (MQ=255)                                                       gCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCg                                                                              <  2:54454/140‑1 (MQ=255)                                                         tCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTc                                                                            >  2:467969/1‑140 (MQ=255)                                                         tCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTc                                                                            >  2:810846/1‑140 (MQ=255)                                                              ttGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTc                                                                       <  1:651869/140‑1 (MQ=255)                                                                gAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAGGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCg                                                                     <  1:154240/140‑1 (MQ=255)                                                                gAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCg                                                                     <  2:1454606/140‑1 (MQ=255)                                                                   cAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCa                                                                  <  1:386226/140‑1 (MQ=255)                                                                   cAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCa                                                                  <  2:1121100/140‑1 (MQ=255)                                                                   cAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCa                                                                  >  2:1561787/1‑140 (MQ=255)                                                                     acacAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCatt                                                                >  2:1624833/1‑140 (MQ=255)                                                                      cacaAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCg                                                                              >  1:622121/1‑125 (MQ=255)                                                                      cacaAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCg                                                                              <  2:622121/125‑1 (MQ=255)                                                                      cacaAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCattt                                                               <  1:467969/140‑1 (MQ=255)                                                                      cacaAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCattt                                                               >  1:38248/1‑140 (MQ=255)                                                                      cacaAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCattt                                                               >  2:795228/1‑140 (MQ=255)                                                                        caAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGT‑AAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCatttatt                                                            <  2:6657/140‑1 (MQ=255)                                                                         aaGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCatttatt                                                            <  2:512595/140‑1 (MQ=255)                                                                         aaGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCatttatt                                                            <  2:1049056/140‑1 (MQ=255)                                                                         aaGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCatttatt                                                            <  2:1375361/140‑1 (MQ=255)                                                                         aaGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCatttatt                                                            <  1:1561787/140‑1 (MQ=255)                                                                         aaGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCatttatt                                                            <  1:267551/140‑1 (MQ=255)                                                                         aaGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCatttatt                                                            <  1:1544906/140‑1 (MQ=255)                                                                         aaGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCatttatt                                                            <  2:1335457/140‑1 (MQ=255)                                                                           gCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCAtttattta                                                          >  1:1352865/1‑140 (MQ=255)                                                                           gCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCAtttattta                                                          >  1:1026019/1‑140 (MQ=255)                                                                                tGGTTGCCCGCGTGTTCTCTTGGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTcaa                                                     >  2:1183237/1‑140 (MQ=255)                                                                                 ggTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTcaac                                                    <  1:1374563/140‑1 (MQ=255)                                                                                    tGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCaacaac                                                 >  2:6927/1‑140 (MQ=255)                                                                                    tGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCaacaac                                                 >  2:183913/1‑140 (MQ=255)                                                                                     gCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAacaaca                                                >  2:1170293/1‑140 (MQ=255)                                                                                     gCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAacaaca                                                >  1:821230/1‑140 (MQ=255)                                                                                     gCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAacaaca                                                >  1:1551998/1‑140 (MQ=255)                                                                                                       ccGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCgcagcag                              >  2:805676/1‑140 (MQ=255)                                                                                                        cGGAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCgcagcagc                             >  1:1555297/1‑140 (MQ=255)                                                                                                         ggAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCgcagcagca                            <  1:805676/140‑1 (MQ=255)                                                                                                         ggAGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCgcagcagca                            <  1:1170293/140‑1 (MQ=255)                                                                                                           aGGTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAg                          >  1:463474/1‑140 (MQ=255)                                                                                                             gTAAAAAAAGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGc                        <  2:463474/140‑1 (MQ=255)                                                                                                               aaaaaaaGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCg                      <  1:1624833/140‑1 (MQ=255)                                                                                                                  aaaaGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCGCTg                   <  2:1352865/140‑1 (MQ=255)                                                                                                                  aaaaGAGGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCGCTg                   <  1:183913/140‑1 (MQ=255)                                                                                                                       agGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCGCTGTCCGt              >  1:1512485/1‑140 (MQ=255)                                                                                                                       agGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCGCTGTCCGt              >  2:1362860/1‑140 (MQ=255)                                                                                                                       agGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCGCTGTCCGt              >  1:520227/1‑140 (MQ=255)                                                                                                                       agGATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCGCTGTCCGt              >  2:158601/1‑140 (MQ=255)                                                                                                                       agGATGAGCATAATCCACTTAACCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCGCTGTCCGt              >  1:1480842/1‑140 (MQ=255)                                                                                                                        ggATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCGCTGTCCGTc             <  1:810846/140‑1 (MQ=255)                                                                                                                        ggATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCGCTGTCCGTc             >  1:1096274/1‑140 (MQ=255)                                                                                                                         gATGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCGCTGTCCGTCt            <  1:700505/140‑1 (MQ=255)                                                                                                                           tGAGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCGCTGTCCGTCTGc          <  1:1183237/140‑1 (MQ=255)                                                                                                                           tGAGCATAATCCACTTAACCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCGCTGTCCGTCTGc          <  2:1480842/140‑1 (MQ=255)                                                                                                                             aGCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCGCTGTCCGTCTGCCt        >  2:573888/1‑140 (MQ=255)                                                                                                                              gCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCGCTGTCCGTCTGCCTg       <  1:158601/140‑1 (MQ=255)                                                                                                                              gCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCGCTGTCCGTCTGCCTg       <  2:1096274/140‑1 (MQ=255)                                                                                                                              gCATAATCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCGCTGTCCGTCTGCCTg       <  2:821230/140‑1 (MQ=255)                                                                                                                                  aaTCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACTGTCCGAAAATCGCAGCAGCAAGGCCGCTGTCCGTCTGCCTGGCgt   <  2:1555297/140‑1 (MQ=255)                                                                                                                                  aaTCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCGCTGTCCGTCTGCCTGGCgt   <  2:1345502/140‑1 (MQ=255)                                                                                                                                   aTCCACTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCGCTGTCCGTCTGCCTGGCgtg  <  2:870570/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        GACCGACTCAACACTACCTTTCGCCAGATGGAACAAGAGCTGGCTATATTTGCCGCTCATCTTGAGCAACACAAGCTATTGGTTGCCCGCGTGTTCTCTTTGCCGGAGGTAAAAAAAGAGGATGAGCATAATCCGCTTAATCGTATTGAGGTAAAACAACATCTCGGCAACGACGCGCAGTCGCTGGCGTTGCGTCATTTCCGCCATTTATTTATTCAACAACAGTCCGAAAATCGCAGCAGCAAGGCCGCTGTCCGTCTGCCTGGCGTG  >  NC_000913/1684280‑1684549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑