Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,760,996	T→C	S266G (AGC→GGC)	sufD ←	Fe‑S cluster scaffold complex subunit SufD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,760,996	0	T	C	100.0%	228.6 / NA	65	S266G (AGC→GGC)	sufD	Fe‑S cluster scaffold complex subunit SufD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/34);  total (31/34)

CGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGT  >  NC_000913/1760861‑1761127                                                                                                                                        |                                                                                                                                    cgcgGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTTCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGTCAGGCTATTGATCCGCAGCGTGACGttt                                                                                                                                 >  2:478890/1‑140 (MQ=255) cgcgGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACTCCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGttt                                                                                                                                 >  2:1491500/1‑140 (MQ=255) cgcgGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGttt                                                                                                                                 >  2:1408098/1‑140 (MQ=255)            tCGCTGACGATAGTCTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAg                                                                                                                      <  1:676761/140‑1 (MQ=255)               cTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTg                                                                                                                   <  1:1618731/140‑1 (MQ=255)                tGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCg                                                                                                                              >  1:857787/1‑128 (MQ=255)                tGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCg                                                                                                                              <  2:857787/128‑1 (MQ=255)                  aCGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGt                                                                                                                >  2:1253318/1‑140 (MQ=255)                   cGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGTCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTa                                                                                                               <  1:478890/140‑1 (MQ=255)                    gATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTAc                                                                                                              <  1:1491500/140‑1 (MQ=255)                       aGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTgg                                                                                                           >  1:500806/1‑140 (MQ=255)                         ttttGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGgtg                                                                                                         <  1:1253318/140‑1 (MQ=255)                          tttGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGgtgt                                                                                                        <  1:85607/140‑1 (MQ=255)                           ttGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGtgtt                                                                                                       <  1:400165/140‑1 (MQ=255)                            tgtgCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGtgttg                                                                                                      <  2:500806/140‑1 (MQ=255)                            tgtgCAACTGCCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGtgttg                                                                                                      <  1:488998/140‑1 (MQ=255)                              tgCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTtgtg                                                                                                    <  1:1274243/140‑1 (MQ=255)                                 aaCTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCg                                                                                                 <  1:1602294/140‑1 (MQ=255)                                   cTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCATACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTa                                                                                               >  1:354235/1‑140 (MQ=255)                                   cTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTa                                                                                               >  1:1262511/1‑140 (MQ=255)                                    tGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTaa                                                                                              <  2:531476/140‑1 (MQ=255)                                      tCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAaca                                                                                            >  2:861614/1‑140 (MQ=255)                                      tCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAaca                                                                                            >  2:1145820/1‑140 (MQ=255)                                                cAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCAcc                                                                                  <  1:1260801/140‑1 (MQ=255)                                                cAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCAcc                                                                                  <  1:1408098/140‑1 (MQ=255)                                                cAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCAcc                                                                                  <  1:55857/140‑1 (MQ=255)                                                        tttATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGa                                                                          >  2:1578474/1‑140 (MQ=255)                                                          tATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGaaa                                                                        >  1:396216/1‑140 (MQ=255)                                                               tgtTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCa                                                                                    <  2:472833/123‑1 (MQ=255)                                                               tgtTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCa                                                                                    >  1:472833/1‑123 (MQ=255)                                                               tgtTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACtgtg                                                                   <  1:1578474/140‑1 (MQ=255)                                                               tgtTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACtgtg                                                                   <  1:1145820/140‑1 (MQ=255)                                                                 ttCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGc                                                                 >  1:845176/1‑140 (MQ=255)                                                                    cagccagGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTaa                                                              >  2:1320247/1‑140 (MQ=255)                                                                      gccagGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAAt                                                            >  2:22320/1‑140 (MQ=255)                                                                      gccagGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAAt                                                            >  2:479579/1‑140 (MQ=255)                                                                      gccagGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAAt                                                            >  2:708037/1‑140 (MQ=255)                                                                           gTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGt                                                       <  2:496382/140‑1 (MQ=255)                                                                                ggTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGTTGGCac                                                  >  2:698934/1‑139 (MQ=255)                                                                                    tCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTc                                              >  1:46083/1‑140 (MQ=255)                                                                                     cacacaCCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTca                                             >  2:1203793/1‑140 (MQ=255)                                                                                     cacacaCCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTca                                             >  1:242413/1‑140 (MQ=255)                                                                                     cacacaCCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTca                                             >  2:95852/1‑140 (MQ=255)                                                                                       cacacCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTcagc                                           >  2:1430225/1‑140 (MQ=255)                                                                                         cacCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTcagcca                                         <  1:479579/140‑1 (MQ=255)                                                                                          acCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTcagc                                           >  2:991488/1‑137 (MQ=255)                                                                                          acCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTcagc                                           <  1:991488/137‑1 (MQ=255)                                                                                            cTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGca                                      <  2:1262511/140‑1 (MQ=255)                                                                                              cGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGcaac                                    <  1:22320/140‑1 (MQ=255)                                                                                              cGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGcaac                                    <  1:708037/140‑1 (MQ=255)                                                                                                         ggCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTa                           >  1:598574/1‑138 (MQ=255)                                                                                                         ggCATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTa                           <  2:598574/138‑1 (MQ=255)                                                                                                           cATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAg                       >  2:821568/1‑140 (MQ=255)                                                                                                           cATCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCACACAGCAACAAATCGTTATGAg                       >  2:626239/1‑140 (MQ=255)                                                                                                            aTCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGc                      <  2:396216/140‑1 (MQ=255)                                                                                                             tCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCa                     <  2:354235/140‑1 (MQ=255)                                                                                                             tCGCCAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCa                     <  1:1430225/140‑1 (MQ=255)                                                                                                                ccAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGc                      <  1:104506/136‑1 (MQ=255)                                                                                                                ccAGGCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGc                      >  2:104506/1‑136 (MQ=255)                                                                                                                    gCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAgtggt              <  1:861614/140‑1 (MQ=255)                                                                                                                    gCTATTGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAgtggt              <  2:1079301/140‑1 (MQ=255)                                                                                                                        ttGATCCGCAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACt          >  2:1017775/1‑140 (MQ=255)                                                                                                                                cAGCGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGt  >  1:1110591/1‑140 (MQ=255)                                                                                                                                   cGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAg       >  1:20707/1‑132 (MQ=255)                                                                                                                                   cGTGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAg       <  2:20707/132‑1 (MQ=255)                                                                                                                                     tGCCGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGt  <  2:642973/135‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    CGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGT  >  NC_000913/1760861‑1761127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑