Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,793,268	A→C	H97Q (CAT→CAG)	btuD ←	vitamin B12 ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,793,268	0	A	C	100.0%	271.2 / NA	76	H97Q (CAT→CAG)	btuD	vitamin B12 ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (37/39);  total (37/39)

TGCAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTGAATGCTTCCCTTAC  >  NC_000913/1793131‑1793404                                                                                                                                          |                                                                                                                                         tGCAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGctgctg                                                                                                                                        >  1:1507350/1‑140 (MQ=255)   cAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCa                                                                                                                                      <  1:248830/140‑1 (MQ=255)   cAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCa                                                                                                                                      <  1:377867/140‑1 (MQ=255)   cAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCa                                                                                                                                      >  2:273913/1‑140 (MQ=255)      caccacCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAgtg                                                                                                                                   >  2:273302/1‑140 (MQ=255)        ccaccGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTc                                                                                                                                 >  2:232133/1‑140 (MQ=255)        ccaccGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATAGTGCTGCTGCAGTGTc                                                                                                                                 >  1:1076190/1‑140 (MQ=255)         caccGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCa                                                                                                                                >  1:728821/1‑140 (MQ=255)           ccGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGctgctg                                                                                                                                        >  1:381847/1‑130 (MQ=255)            cGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGt                                                                                                                             >  2:1415683/1‑140 (MQ=255)             gcagcaAGACGTACGCGTTGCCATTCGCCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTa                                                                                                                            <  1:84904/140‑1 (MQ=255)                 caAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGc                                                                                                                        >  2:1405317/1‑140 (MQ=255)                   aGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGctgctg                                                                                                                                        >  1:29272/1‑122 (MQ=255)                   aGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCa                                                                                                                      >  2:1317034/1‑140 (MQ=255)                   aGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCa                                                                                                                      >  1:233901/1‑140 (MQ=255)                      cGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGAc                                                                                                                   >  1:1624144/1‑140 (MQ=255)                      cGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGAc                                                                                                                   <  1:264797/140‑1 (MQ=255)                      cGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGAc                                                                                                                   >  1:639093/1‑140 (MQ=255)                      cGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGAc                                                                                                                   <  2:639093/140‑1 (MQ=255)                      cGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGAc                                                                                                                   >  1:1421763/1‑140 (MQ=255)                      cGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGAc                                                                                                                   <  2:728821/140‑1 (MQ=255)                         aCGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCgg                                                                                                                <  1:732829/140‑1 (MQ=255)                         aCGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCgg                                                                                                                >  1:1408907/1‑140 (MQ=255)                           gcgTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATAGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCg                                                                                                              <  2:1076190/140‑1 (MQ=255)                              ttGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTg                                                                                                           >  2:290152/1‑140 (MQ=255)                              ttGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTg                                                                                                           >  2:1621624/1‑140 (MQ=255)                               tGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGc                                                                                                          >  2:1315601/1‑140 (MQ=255)                                gCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGCACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCa                                                                                                         >  1:1505656/1‑140 (MQ=255)                                       aCCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAAcggcg                                                                                                  <  1:273302/140‑1 (MQ=255)                                       aCCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAAcggcg                                                                                                  <  2:1214413/140‑1 (MQ=255)                                         cgccgGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACggcggc                                                                                                >  1:1542967/1‑140 (MQ=255)                                         cgccgGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACggcggc                                                                                                >  1:1312078/1‑140 (MQ=255)                                           ccgGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGTGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGt                                                                                              >  1:427276/1‑140 (MQ=255)                                             ggAAAGTTGATTGGTGCTTCGTCCGAGTTTGTCATCAAGAGGCAGCGCCCCTGCGCCATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTct                                                                                            <  1:1180496/140‑1 (MQ=255)                                                aaGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgct                                                                                         <  1:1405317/140‑1 (MQ=255)                                                 aGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgctg                                                                                        <  2:1507350/140‑1 (MQ=255)                                                 aGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgctg                                                                                        <  2:1288625/140‑1 (MQ=255)                                                      atcggTGCTACGTCCGAGTTTGTCATCAAGCGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTtgtg                                                                                   <  2:1019095/137‑1 (MQ=255)                                                       ttGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGa                                                                                  <  1:1415683/140‑1 (MQ=255)                                                           tGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGa                                                                              >  1:1158746/1‑140 (MQ=255)                                                            gCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGAt                                                                             <  2:1154510/140‑1 (MQ=255)                                                                cGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGgc                                                                         >  1:836830/1‑140 (MQ=255)                                                                 gTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGgcg                                                                        <  2:836830/140‑1 (MQ=255)                                                                     gAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgttg                                                                                     <  1:1062540/123‑1 (MQ=255)                                                                     gAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgttg                                                                                     >  2:1062540/1‑123 (MQ=255)                                                                          tGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAgc                                                               >  2:1013850/1‑140 (MQ=255)                                                                          tGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGCACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCGGACCGGCGTTGCAAACGGCGGCGTCCGCTGTTGTGAAAGATAGGCGCGATGCAgc                                                               <  2:1505656/140‑1 (MQ=255)                                                                            tcatcaAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAATGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAgcgc                                                             >  2:1512889/1‑140 (MQ=255)                                                                             catcaAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGcgcg                                                            <  1:1512889/140‑1 (MQ=255)                                                                               tcaAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAg                                                          <  2:1542967/140‑1 (MQ=255)                                                                                       cAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTttgttg                                                  <  2:1155405/140‑1 (MQ=255)                                                                                        aGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGc                                                 >  2:763951/1‑140 (MQ=255)                                                                                        aGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGc                                                 >  1:777934/1‑140 (MQ=255)                                                                                          cgcCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAgc                                                               >  2:325275/1‑124 (MQ=255)                                                                                          cgcCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAgc                                                               <  1:325275/124‑1 (MQ=255)                                                                                          cgcCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCgg                                               >  1:23786/1‑140 (MQ=255)                                                                                            ccccTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGAc                                             <  2:233901/140‑1 (MQ=255)                                                                                                tGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATg                                         <  2:1408907/140‑1 (MQ=255)                                                                                                            cAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGtt                             <  2:922041/140‑1 (MQ=255)                                                                                                            cAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGtt                             <  2:1238970/140‑1 (MQ=255)                                                                                                               tagtTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGcc                          >  1:995400/1‑140 (MQ=255)                                                                                                                  ttCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCcg                       >  2:947365/1‑140 (MQ=255)                                                                                                                  ttCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCcg                       <  1:947365/140‑1 (MQ=255)                                                                                                                  ttCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCcg                       <  2:1312078/140‑1 (MQ=255)                                                                                                                    cGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCcgcg                     >  1:495915/1‑140 (MQ=255)                                                                                                                    cGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCcgcg                     >  1:487018/1‑140 (MQ=255)                                                                                                                    cGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCcgcg                     >  1:543375/1‑140 (MQ=255)                                                                                                                    cGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCcgcg                     >  2:833380/1‑140 (MQ=255)                                                                                                                    cGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCcgcg                     >  1:1221780/1‑140 (MQ=255)                                                                                                                       tACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAAc                  <  1:290152/140‑1 (MQ=255)                                                                                                                       tACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAAc                  <  2:543375/140‑1 (MQ=255)                                                                                                                        aCGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTCTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACt                 <  2:1158746/140‑1 (MQ=255)                                                                                                                         cgcgTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTg                >  2:113899/1‑140 (MQ=255)                                                                                                                           cgTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTGaa              <  1:763951/140‑1 (MQ=255)                                                                                                                           cgTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTGaa              <  1:1013850/140‑1 (MQ=255)                                                                                                                             ttttATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTGAATg            <  2:1221780/140‑1 (MQ=255)                                                                                                                             ttttATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTGAATg            <  2:495915/140‑1 (MQ=255)                                                                                                                               ttATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTGAATGCt          <  2:1114774/140‑1 (MQ=255)                                                                                                                               ttATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTGAATGCt          <  2:1421763/140‑1 (MQ=255)                                                                                                                                      gctgctGCAGTGTCAGGT‑GTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTGAATGCTTCCCTTAc  >  2:350005/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         TGCAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTGAATGCTTCCCTTAC  >  NC_000913/1793131‑1793404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑