BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,100,008	A→G	F421L (TTC→CTC)	gnd ←	6‑phosphogluconate dehydrogenase, decarboxylating

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,100,008	0	A	G	100.0%	466.6 / NA	130	F421L (TTC→CTC)	gnd	6‑phosphogluconate dehydrogenase, decarboxylating
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (70/60); total (70/60)

Only 100 of 132 total aligned reads displayed.

CCATTCGGTATGGAACACACCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAA  >  NC_000913/2099871‑2100138

ccATTCGGTATGGAACACACCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAggg                                                                                                                                  >  1:1590255/1‑140 (MQ=255)

    tCGGTATGGAACTCACCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCgg                                                                                                                              <  1:746703/140‑1 (MQ=255)

     cGGTATGGAACACACCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGa                                                                                                                             >  1:855996/1‑140 (MQ=255)

       gTATGGAACACACCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAAc                                                                                                                           <  2:1141673/140‑1 (MQ=255)

             aaCACACCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAAt                                                                                                                     >  1:1180931/1‑140 (MQ=255)

              acacacCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATa                                                                                                                    <  1:368282/140‑1 (MQ=255)

               cacacCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATAc                                                                                                                   >  2:330732/1‑140 (MQ=255)

                  acCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATAcc                                                                                                                  <  1:41097/138‑1 (MQ=255)

                  acCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATAcc                                                                                                                  >  2:41097/1‑138 (MQ=255)

                  acCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGt                                                                                                                >  2:1373761/1‑140 (MQ=255)

                  acCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGt                                                                                                                >  2:1349740/1‑140 (MQ=255)

                  acCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGt                                                                                                                >  2:214670/1‑140 (MQ=255)

                  acCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGt                                                                                                                >  1:1318835/1‑140 (MQ=255)

                  acCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGt                                                                                                                >  1:1237152/1‑140 (MQ=255)

                  acCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGt                                                                                                                >  1:317873/1‑140 (MQ=255)

                   ccTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGtt                                                                                                               <  1:1434319/140‑1 (MQ=255)

                   ccTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGtt                                                                                                               <  1:1662847/140‑1 (MQ=255)

                    cttcttTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTc                                                                                                              >  2:549719/1‑140 (MQ=255)

                     ttcttTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCt                                                                                                             <  1:214670/140‑1 (MQ=255)

                      tcttTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTg                                                                                                            >  1:630117/1‑140 (MQ=255)

                      tcttTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTg                                                                                                            <  1:646131/140‑1 (MQ=255)

                      tcttTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTg                                                                                                            >  2:711743/1‑140 (MQ=255)

                       cttTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGt                                                                                                           >  1:1594514/1‑140 (MQ=255)

                       cttTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGt                                                                                                           <  2:1594514/140‑1 (MQ=255)

                       cttTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGt                                                                                                           <  1:1373761/140‑1 (MQ=255)

                       cttTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGt                                                                                                           >  1:707626/1‑140 (MQ=255)

                        tttATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTa                                                                                                          <  2:317873/140‑1 (MQ=255)

                          tATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACt                                                                                                        >  2:1066527/1‑140 (MQ=255)

                            tCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGc                                                                                                      >  1:608211/1‑140 (MQ=255)

                            tCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGc                                                                                                      >  2:492491/1‑140 (MQ=255)

                             cAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCa                                                                                                     <  1:1066527/140‑1 (MQ=255)

                              aaTACGCTTATAAGTATGCGCACCAAAATAGTCGCGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATa                                                                                                                    >  1:809666/1‑124 (MQ=255)

                              aaTACGCTTATAAGTATGCGCACCAAAATAGTCGCGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATa                                                                                                                    <  2:809666/124‑1 (MQ=255)

                              aaTACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCAt                                                                                                    >  2:120550/1‑140 (MQ=255)

                                tACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATaa                                                                                                  >  2:552698/1‑140 (MQ=255)

                                  cGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGc                                                                                                >  1:774711/1‑140 (MQ=255)

                                    cTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCaa                                                                                              <  1:738425/140‑1 (MQ=255)

                                      tataAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACg                                                                                            >  2:587306/1‑140 (MQ=255)

                                      tataAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACg                                                                                            >  2:1236110/1‑140 (MQ=255)

                                        taAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGAc                                                                                          >  2:521118/1‑140 (MQ=255)

                                           gTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATc                                                                                       <  2:774711/140‑1 (MQ=255)

                                             aTGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCAc                                                                                     >  2:157131/1‑140 (MQ=255)

                                             aTGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCAc                                                                                     >  1:1081866/1‑140 (MQ=255)

                                              tGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACg                                                                                    >  1:1534614/1‑140 (MQ=255)

                                              tGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACg                                                                                    <  2:392095/140‑1 (MQ=255)

                                              tGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACg                                                                                    >  1:410149/1‑140 (MQ=255)

                                               gcgcACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGc                                                                                   >  1:588815/1‑140 (MQ=255)

                                                 gcACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAg                                                                                 <  1:711743/140‑1 (MQ=255)

                                                  cACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAgc                                                                                >  1:1535897/1‑140 (MQ=255)

                                                   aCCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAgcg                                                                               <  1:157131/140‑1 (MQ=255)

                                                    ccAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAgcgc                                                                              <  1:120550/140‑1 (MQ=255)

                                                      aaaaTAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCct                                                                            <  1:330732/140‑1 (MQ=255)

                                                        aaTAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCctgc                                                                          <  1:1085740/140‑1 (MQ=255)

                                                           aGTCAAGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTgg                                                                       <  1:341084/140‑1 (MQ=255)

                                                             tCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTa                                                                     >  2:1443974/1‑140 (MQ=255)

                                                               aCGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGt                                                                   <  2:588815/140‑1 (MQ=255)

                                                                  cTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCAt                                                                >  1:1191524/1‑140 (MQ=255)

                                                                  cTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCAt                                                                >  1:453257/1‑140 (MQ=255)

                                                                     tgCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGt                                                                      <  1:745867/131‑1 (MQ=255)

                                                                     tgCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGt                                                                      >  2:745867/1‑131 (MQ=255)

                                                                     tgCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCgg                                                             >  2:1629587/1‑140 (MQ=255)

                                                                       ccTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCa                                                           >  2:1931/1‑140 (MQ=255)

                                                                        cTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCaa                                                          <  1:587306/140‑1 (MQ=255)

                                                                          ggATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAAtt                                                        >  1:699020/1‑140 (MQ=255)

                                                                           gATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAAttt                                                       >  2:398057/1‑140 (MQ=255)

                                                                           gATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAAttt                                                       >  2:829448/1‑140 (MQ=255)

                                                                             tCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGc                                                     <  1:1443974/140‑1 (MQ=255)

                                                                             tCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGc                                                     <  2:1081866/140‑1 (MQ=255)

                                                                             tCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGc                                                     <  1:552698/140‑1 (MQ=255)

                                                                               aGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCtt                                                   <  2:1535897/140‑1 (MQ=255)

                                                                                ggTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTg                                                  <  1:507514/140‑1 (MQ=255)

                                                                                 gTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGa                                                 >  2:1109666/1‑140 (MQ=255)

                                                                                     gcaggcagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTa                                             >  2:841000/1‑140 (MQ=255)

                                                                                     gcaggcagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTa                                             <  1:1931/140‑1 (MQ=255)

                                                                                      caggcagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTAc                                            >  1:2610/1‑140 (MQ=255)

                                                                                          cagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAg                                        <  1:398057/140‑1 (MQ=255)

                                                                                          cagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAg                                        <  1:829448/140‑1 (MQ=255)

                                                                                          cagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGCCAATTTGCTTGAAGTACGGAg                                        <  2:1180931/140‑1 (MQ=255)

                                                                                           agAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGc                                       >  1:477622/1‑140 (MQ=255)

                                                                                                 gcagcaCGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCaa                                 <  1:976486/140‑1 (MQ=255)

                                                                                                 gcagcaCGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCaa                                 <  2:453257/140‑1 (MQ=255)

                                                                                                 gcagcaCGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCaa                                 <  1:1629587/140‑1 (MQ=255)

                                                                                                   agcaCGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACa                               <  2:590250/140‑1 (MQ=255)

                                                                                                   agcaCGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACa                               >  1:46441/1‑140 (MQ=255)

                                                                                                       cGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGtt                           <  2:1237152/140‑1 (MQ=255)

                                                                                                         gTAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAg                         <  1:521118/140‑1 (MQ=255)

                                                                                                          tAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGc                        >  1:1003912/1‑140 (MQ=255)

                                                                                                          tAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGc                        <  2:71926/140‑1 (MQ=255)

                                                                                                          tAGCTGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGc                        <  1:151152/140‑1 (MQ=255)

                                                                                                              tGTCGTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATc                    <  2:775474/140‑1 (MQ=255)

                                                                                                                  gTAATAGGCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCtgtg                >  1:539238/1‑140 (MQ=255)

                                                                                                                        ggCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGAtttt          <  2:1562430/140‑1 (MQ=255)

                                                                                                                        ggCAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGAtttt          <  2:46441/140‑1 (MQ=255)

                                                                                                                          cAACCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCg        >  1:371644/1‑140 (MQ=255)

                                                                                                                            aCCGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGc      >  1:36756/1‑140 (MQ=255)

                                                                                                                             ccGCTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCa     >  2:764914/1‑140 (MQ=255)

                                                                                                                                cTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATaa  >  1:1246401/1‑140 (MQ=255)

                                                                                                                                cTGCGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATaa  >  1:206204/1‑140 (MQ=255)

                                                                                                                                   cGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCg        <  2:234267/131‑1 (MQ=255)

                                                                                                                                   cGGAGAGGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCg        >  1:234267/1‑131 (MQ=255)

CCATTCGGTATGGAACACACCTTCTTTATCAATACGCTTATAAGTATGCGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAA  >  NC_000913/2099871‑2100138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`