Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,241,995	G→C	H325D (CAC→GAC)	yeiB ←	DUF418 domain‑containing protein YeiB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,241,995	0	G	C	100.0%	227.9 / NA	64	H325D (CAC→GAC)	yeiB	DUF418 domain‑containing protein YeiB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (31/33);  total (31/33)

CGCCAGAGCCATTCCACCGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCA  >  NC_000913/2241862‑2242131                                                                                                                                      |                                                                                                                                         cGCCAGAGCCATTCCACCGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTaaaa                                                                                                                                    >  1:945225/1‑140 (MQ=255)  gCCAGAGCCATTCCACCGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTaaaaa                                                                                                                                   >  1:1155570/1‑140 (MQ=255)    cAGAGCCATTCCACCGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAg                                                                                                                                 <  2:658150/140‑1 (MQ=255)    cAGAGCCATTCCACCGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAg                                                                                                                                 <  1:1005357/140‑1 (MQ=255)    cAGAGCCATTCCACCGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAg                                                                                                                                 <  2:1266504/140‑1 (MQ=255)      gagCCATTCCACCGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCg                                                                                                                               <  1:1172192/140‑1 (MQ=255)      gagCCATTCCACCGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCg                                                                                                                               <  2:118610/140‑1 (MQ=255)      gagCCATTCCACCGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCg                                                                                                                               <  1:552170/140‑1 (MQ=255)      gagCCATTCCACCGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCg                                                                                                                               <  2:426675/140‑1 (MQ=255)            ttCCACCGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGt                                                                                                                           >  2:799223/1‑138 (MQ=255)            ttCCACCGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGt                                                                                                                           <  1:799223/138‑1 (MQ=255)             tCCACCGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACa                                                                                                                        <  1:879420/140‑1 (MQ=255)                  cGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCa                                                                                                                   <  2:261020/140‑1 (MQ=255)                    gCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGc                                                                                                                 <  2:564794/140‑1 (MQ=255)                      cccTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGt                                                                                                               <  1:492893/140‑1 (MQ=255)                        cTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGttt                                                                                                             <  1:1031132/140‑1 (MQ=255)                         tGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTg                                                                                                            <  2:556700/140‑1 (MQ=255)                                aGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAataga                                                                                                     <  1:1406899/140‑1 (MQ=255)                                aGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAAGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGc                                                                                                                 >  2:498617/1‑128 (MQ=255)                                aGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAAGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGc                                                                                                                 <  1:498617/128‑1 (MQ=255)                                   aaCGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAatagatag                                                                                                  <  2:613423/140‑1 (MQ=255)                                    aCGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGt                                                                                                 >  2:1440210/1‑140 (MQ=255)                                            aGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAata                                                                                                       >  2:1356009/1‑126 (MQ=255)                                            aGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAata                                                                                                       <  1:1356009/126‑1 (MQ=255)                                                aaCAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCa                                                                                     <  1:197408/140‑1 (MQ=255)                                                    gagaAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCAtccg                                                                                 <  2:1155570/140‑1 (MQ=255)                                                      gaagaGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCAtccgtc                                                                               <  1:1057133/140‑1 (MQ=255)                                                       aagaGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCGGCGTTTGCAATAGATAGTTGGTCAGCGCCAtccgtcc                                                                              >  1:1161756/1‑140 (MQ=255)                                                       aagaGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGt                                                                                             <  1:582876/125‑1 (MQ=255)                                                       aagaGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGt                                                                                             >  2:582876/1‑125 (MQ=255)                                                       aagaGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCAtccgtcc                                                                              >  2:1092410/1‑140 (MQ=255)                                                        agagGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCAtccgtccg                                                                             >  1:1319074/1‑140 (MQ=255)                                                        agagGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCAtccgtccg                                                                             >  1:393423/1‑140 (MQ=255)                                                                 tCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCg                                                                    >  1:980059/1‑140 (MQ=255)                                                                    ccagccaTACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATc                                                                 >  1:609635/1‑140 (MQ=255)                                                                      agccaTACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc                                                               >  1:589779/1‑140 (MQ=255)                                                                           tACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCa                                                          >  2:541936/1‑140 (MQ=255)                                                                           tACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCa                                                          <  2:498272/140‑1 (MQ=255)                                                                            aCCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCAc                                                         <  2:393423/140‑1 (MQ=255)                                                                                     aCAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTaaa                                                >  1:446899/1‑140 (MQ=255)                                                                                      cAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCGGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAg                                               <  2:1161756/140‑1 (MQ=255)                                                                                      cAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCa                                                              >  2:251413/1‑125 (MQ=255)                                                                                      cAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCa                                                              <  1:251413/125‑1 (MQ=255)                                                                                      cAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAg                                               >  1:483624/1‑140 (MQ=255)                                                                                          cGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc                                           >  1:478652/1‑140 (MQ=255)                                                                                           gCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCt                                          >  2:1451056/1‑140 (MQ=255)                                                                                               gcagcTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAAt                                      >  2:1613656/1‑140 (MQ=255)                                                                                               gcagcTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAAt                                      >  1:670312/1‑140 (MQ=255)                                                                                                        ggCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAg                             >  1:257186/1‑140 (MQ=255)                                                                                                         gCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGa                            <  2:478652/140‑1 (MQ=255)                                                                                                         gCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGa                            <  2:483624/140‑1 (MQ=255)                                                                                                         gCGGTCAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGa                            <  1:1451056/140‑1 (MQ=255)                                                                                                              cAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCa                       <  2:609635/140‑1 (MQ=255)                                                                                                              cAAAATGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCa                       >  1:1126761/1‑140 (MQ=255)                                                                                                                aaaTGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATa                     <  2:945225/140‑1 (MQ=255)                                                                                                                   tGCATAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATaaaa                  >  2:992532/1‑140 (MQ=255)                                                                                                                       tAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGc              >  2:264566/1‑140 (MQ=255)                                                                                                                       tAAACAAACCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGc              >  1:1285529/1‑140 (MQ=255)                                                                                                                        aaacaaacCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCTGCCAGAAGCCATAAAACAGCg             <  1:1092410/140‑1 (MQ=255)                                                                                                                           caaacCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACg          <  1:1338630/140‑1 (MQ=255)                                                                                                                              acCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCAt       >  2:363421/1‑140 (MQ=255)                                                                                                                              acCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCAt       >  2:1182895/1‑140 (MQ=255)                                                                                                                              acCGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCAt       >  2:1103647/1‑140 (MQ=255)                                                                                                                                cGAGGTCGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACACATAg     >  2:891620/1‑140 (MQ=255)                                                                                                                                   ggtcgtAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCa  <  2:446899/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         CGCCAGAGCCATTCCACCGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCA  >  NC_000913/2241862‑2242131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑