| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,302,575 | T→C | Q60R (CAG→CGG) | napA ← | periplasmic nitrate reductase subunit NapA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,302,575 | 0 | T | C | 95.9% | 160.2 / ‑2.4 | 49 | Q60R (CAG→CGG) | napA | periplasmic nitrate reductase subunit NapA |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (24/23); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTCATACGCAGCAGCGGCTGCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCTGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGC > NC_000913/2302439‑2302708 | tttCATACGCAGCAGCGGCTGCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCcgcg > 2:1611550/1‑140 (MQ=255)tttCATACGCAGCAGCGGCTGCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCcgcg > 2:288619/1‑140 (MQ=255) tCATACGCAGCAGCGGCTGCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGtt < 1:676171/140‑1 (MQ=255) cGCAGCAGCGGCTGCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGAc < 2:814983/140‑1 (MQ=255) cagcGGCTGCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGaa > 1:831730/1‑140 (MQ=255) agcGGCTGCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAAc > 1:900807/1‑140 (MQ=255) ggCTGCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAAcgcc < 1:1611550/140‑1 (MQ=255) ggCTGCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAAcgcc < 2:659352/140‑1 (MQ=255) gCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCTGCGTTCCGACCAGAACGCCGCaa > 1:1548021/1‑140 (MQ=255) gCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTcc > 1:77858/1‑124 (MQ=255) gCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTcc < 2:77858/124‑1 (MQ=255) gCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCaa > 1:601196/1‑140 (MQ=255) tCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCa > 2:1393133/1‑140 (MQ=255) cAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAg < 1:1393133/140‑1 (MQ=255) aCGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTAc > 1:305821/1‑140 (MQ=255) tCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAAcgcc > 2:1392725/1‑125 (MQ=255) tCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAAcgcc < 1:1392725/125‑1 (MQ=255) tCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCa > 1:1412068/1‑140 (MQ=255) cTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAg > 1:372307/1‑140 (MQ=255) tttACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGa > 2:830861/1‑140 (MQ=255) aCATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAacggcac > 1:548727/1‑140 (MQ=255) cATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAacggcacg < 2:1412068/140‑1 (MQ=255) cATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAacggcacg < 2:900807/140‑1 (MQ=255) aTGATTTTGGGCAGGAGATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAacggcacg > 1:1641496/1‑139 (MQ=255) aTGATTTTGGGCAGGAGATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAacggcacg < 2:1641496/139‑1 (MQ=255) ttttGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCt < 2:548727/140‑1 (MQ=255) ttttGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCt < 2:372307/140‑1 (MQ=255) tttGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCtt < 2:1651906/140‑1 (MQ=255) aaaTAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAacggcacgg > 2:680293/1‑125 (MQ=255) aaaTAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAacggcacgg < 1:680293/125‑1 (MQ=255) aTAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGa < 1:830861/140‑1 (MQ=255) aTGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCctg < 1:144652/140‑1 (MQ=255) aTGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCctg < 2:601196/140‑1 (MQ=255) aGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTAT‑CCATTTGATGGCTTCCTGCTGAc < 2:305821/140‑1 (MQ=255) gTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGAc > 2:200438/1‑140 (MQ=255) ttCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGAcc > 1:752396/1‑140 (MQ=255) tCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGACca > 1:1565464/1‑140 (MQ=255) cAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGACcaa > 1:81271/1‑140 (MQ=255) gCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCctgc < 2:354473/130‑1 (MQ=255) gCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCctgc > 1:354473/1‑130 (MQ=255) gCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGACcaacaa > 2:734693/1‑140 (MQ=255) cGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACAGGTCCCTGCTGCGTTCCGACCAGAACGCCGCAATCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGACCAACAACGgc < 2:1548021/140‑1 (MQ=255) ggTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGACCAACAACGgcg > 1:212357/1‑140 (MQ=255) ggTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGACCAACAACGgcg > 2:635170/1‑140 (MQ=255) ggTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGACCAACAACGgcg > 1:550096/1‑140 (MQ=255) aCCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGACCAACAACGGCGCGGGc < 2:752396/140‑1 (MQ=255) tGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGACCAACAACGGCGCGGGCAACGc < 2:212357/140‑1 (MQ=255) cGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGACCAACAACGGCGCGGGCAACGccc > 1:1032088/1‑140 (MQ=255) cGCCCTGACAGGCCACCACACGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCACGCTg < 1:200438/140‑1 (MQ=255) cacGTCCCTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCa < 1:726784/140‑1 (MQ=255) cccTGCCGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGc > 1:1095638/1‑140 (MQ=255) | TTTCATACGCAGCAGCGGCTGCGTCAAACGGTCTTTACCGTACATGATTTTGGGCAGGAAATAGCCCTTAATGCAGTTCAGGCCACGGTTAACCGGTGCGTCCGGGTCGCCCTGACAGGCCACCACACGTCCCTGCTGCGTTCCGACCAGAACGCCGCAACCAGTACCGCAGAAACGGCACGGCGCTTTATCCCATTTGATGGCTTCCTGCTGACCAACAACGGCGCGGGCAACGCCCGGCACGCTGAGACCGGCAGCCGCCGCAGCGGC > NC_000913/2302439‑2302708 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |