Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	214,389	T→C	P33P (CCT→CCC)	yaeQ →	uncharacterized protein YaeQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	214,389	0	T	C	100.0%	292.4 / NA	81	P33P (CCT→CCC)	yaeQ	uncharacterized protein YaeQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/41);  total (40/41)

GTTACAATGGCCTCCTGATTCGAAAGGAGTTTTCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCTTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAACGATCACC  >  NC_000913/214255‑214516                                                                                                                                       |                                                                                                                                gTTACAATGGCCTCCTGATTCGAAAGGAGTTTTCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGa                                                                                                                            >  2:1608454/1‑140 (MQ=255) gTTACAATGGCCTCCTGATTCGAAAGGAGTTTTCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGa                                                                                                                            >  1:1642712/1‑140 (MQ=255)  ttACAATGGCCTCCTGATTCGAAAGGAGTTTTCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGaa                                                                                                                           >  2:1515834/1‑140 (MQ=255)       aTGGCCTCCTGATTCGAAAGGAGTTTTCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCa                                                                                                                      <  1:441348/140‑1 (MQ=255)        tGGCCTCCTGATTCGAAAGGAGTTTTCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAg                                                                                                                     >  2:1053910/1‑140 (MQ=255)        tGGCCTCCTGATTCGAAAGGAGTTTTCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAg                                                                                                                     >  2:469699/1‑140 (MQ=255)            ctcctGATTCGAAAGGAGTTTTCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGc                                                                                                                 <  2:1583486/140‑1 (MQ=255)               ctGATTCGAAAGGAGTTTTCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAg                                                                                                                     <  1:100797/133‑1 (MQ=255)               ctGATTCGAAAGGAGTTTTCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAg                                                                                                                     >  2:100797/1‑133 (MQ=255)               ctGATTCGAAAGGAGTTTTCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTa                                                                                                              >  2:1628336/1‑140 (MQ=255)               ctGATTCGAAAGGAGTTTTCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTa                                                                                                              <  2:688281/140‑1 (MQ=255)               ctGATTCGAAAGGAGTTTTCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTa                                                                                                              >  1:688281/1‑140 (MQ=255)                    tCGAAAGGAGTTTTCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTatgatg                                                                                                         <  1:1053910/140‑1 (MQ=255)                             gTTTTCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTg                                                                                                >  1:549678/1‑140 (MQ=255)                             gTTTTCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTg                                                                                                <  2:370977/140‑1 (MQ=255)                                ttCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTa                                                                                                              <  2:1247102/123‑1 (MQ=255)                                ttCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTa                                                                                                              >  1:1247102/1‑123 (MQ=255)                                  cTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGc                                                                                           <  1:1628336/140‑1 (MQ=255)                                      tGGCGCTTAAAGCGACAATTTATAAAGCGACAGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTgg                                                                                       <  2:233969/140‑1 (MQ=255)                                         cgcTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTgg                                                                                       >  2:511304/1‑137 (MQ=255)                                         cgcTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTgg                                                                                       <  1:511304/137‑1 (MQ=255)                                         cgcTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTg                                                                                    <  2:1537116/140‑1 (MQ=255)                                           cTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCg                                                                                          <  1:1378388/132‑1 (MQ=255)                                           cTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCg                                                                                          >  2:1378388/1‑132 (MQ=255)                                           cTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGaa                                                                                  >  1:82614/1‑140 (MQ=255)                                           cTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGaa                                                                                  <  2:1642712/140‑1 (MQ=255)                                           cTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGaa                                                                                  <  1:469699/140‑1 (MQ=255)                                           cTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGaa                                                                                  <  1:774067/140‑1 (MQ=255)                                             tAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGa                                                                                   >  1:220318/1‑137 (MQ=255)                                             tAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGa                                                                                   <  2:220318/137‑1 (MQ=255)                                             tAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAat                                                                                <  2:181614/140‑1 (MQ=255)                                             tAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAat                                                                                >  1:181614/1‑140 (MQ=255)                                                    aCAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCtga                                                                         >  1:660586/1‑140 (MQ=255)                                                    aCAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCtga                                                                         >  1:1174326/1‑140 (MQ=255)                                                      aaTTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCtgatg                                                                       >  2:1137888/1‑140 (MQ=255)                                                        tttATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGaa                                                                     >  2:61182/1‑140 (MQ=255)                                                         ttATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAAc                                                                    <  2:1583517/140‑1 (MQ=255)                                                         ttATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAAc                                                                    <  1:367312/140‑1 (MQ=255)                                                           ataAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGt                                                                  >  1:1595935/1‑140 (MQ=255)                                                             aaaGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCt                                                                <  1:399131/140‑1 (MQ=255)                                                                 cGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCaa                                                            <  2:686421/140‑1 (MQ=255)                                                                   aCGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGTTGATGAACGTCTGCAAtt                                                          >  2:232106/1‑140 (MQ=255)                                                                   aCGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAAtt                                                          >  1:587047/1‑140 (MQ=255)                                                                   aCGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAAtt                                                          >  2:787174/1‑140 (MQ=255)                                                                   aCGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAAtt                                                          >  2:1359153/1‑140 (MQ=255)                                                                   aCGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAAtt                                                          >  1:320223/1‑140 (MQ=255)                                                                   aCGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCAATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAAtt                                                          <  2:1482405/140‑1 (MQ=255)                                                                     ggTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTa                                                        <  2:320223/140‑1 (MQ=255)                                                                       ttAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTAcc                                                      <  1:1608454/140‑1 (MQ=255)                                                                                  gATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTtgtg                                           <  2:188461/140‑1 (MQ=255)                                                                                  gATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTtgtg                                           <  2:980862/140‑1 (MQ=255)                                                                                     ctcGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGc                                        <  1:528521/140‑1 (MQ=255)                                                                                         aCCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGt                                                >  1:1277476/1‑128 (MQ=255)                                                                                         aCCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGt                                                <  2:1277476/128‑1 (MQ=255)                                                                                         aCCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGt                                                >  1:1490406/1‑128 (MQ=255)                                                                                         aCCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGAGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGt                                                <  2:1490406/128‑1 (MQ=255)                                                                                            gCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCgatgat                                 <  1:892714/140‑1 (MQ=255)                                                                                             cAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGatgatg                                <  1:1137888/140‑1 (MQ=255)                                                                                              aaCCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGAtgatga                               >  2:1519957/1‑140 (MQ=255)                                                                                                    tttCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGa                         >  2:1393304/1‑140 (MQ=255)                                                                                                    tttCTCGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGa                         <  1:61182/140‑1 (MQ=255)                                                                                                        tcGATGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCg                     <  2:587047/140‑1 (MQ=255)                                                                                                            tGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGc                 >  1:455296/1‑140 (MQ=255)                                                                                                            tGCCTCTCTGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGc                 >  2:514212/1‑140 (MQ=255)                                                                                                                tctctGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCg             <  1:514212/140‑1 (MQ=255)                                                                                                                tctctGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCg             <  2:455296/140‑1 (MQ=255)                                                                                                                tctctGACGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCg             <  2:660586/140‑1 (MQ=255)                                                                                                                    tGACGCTGGCGCGCCATCCCTGAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAAc         >  2:1180651/1‑140 (MQ=255)                                                                                                                      aCGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAACGa       >  2:107849/1‑140 (MQ=255)                                                                                                                      aCGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAACGa       >  2:810494/1‑140 (MQ=255)                                                                                                                      aCGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAACGa       >  1:924881/1‑140 (MQ=255)                                                                                                                       cGCTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAACGAt      <  1:1532622/140‑1 (MQ=255)                                                                                                                         cTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTATCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAACGATCa    >  1:617359/1‑140 (MQ=255)                                                                                                                         cTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCg                     <  1:354097/123‑1 (MQ=255)                                                                                                                         cTGGCGCGCCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCg                     >  2:354097/1‑123 (MQ=255)                                                                                                                            gcgcgcCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACTTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCg             <  2:83237/128‑1 (MQ=255)                                                                                                                            gcgcgcCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACTTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCg             >  1:83237/1‑128 (MQ=255)                                                                                                                            gcgcgcCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCg             >  1:1342546/1‑128 (MQ=255)                                                                                                                            gcgcgcCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCg             <  2:1342546/128‑1 (MQ=255)                                                                                                                            gcgcgcCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAACGATCAcc  >  1:364891/1‑139 (MQ=255)                                                                                                                            gcgcgcCATCCCTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAACGATCAcc  <  2:364891/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                GTTACAATGGCCTCCTGATTCGAAAGGAGTTTTCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCTTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAACGATCACC  >  NC_000913/214255‑214516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑