Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,527,503	T→C	G186G (GGT→GGC)	yfeH →	putative solute:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,527,503	0	T	C	100.0%	342.4 / NA	95	G186G (GGT→GGC)	yfeH	putative solute:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/50);  total (45/50)

TTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTA  >  NC_000913/2527368‑2527639                                                                                                                                        |                                                                                                                                         tttCCTTTCACCATTGCTGGTTGGTCTGGTGATGGATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACt                                                                                                                                      <  2:1014508/140‑1 (MQ=255)        tCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTc                                                                                                                               >  1:1530647/1‑140 (MQ=255)          aCCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTcgc                                                                                                                             >  2:374981/1‑140 (MQ=255)             aTTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCa                                                                                                                          <  1:498076/140‑1 (MQ=255)                    gttggtCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATaaaaa                                                                                                                   >  2:501149/1‑140 (MQ=255)                      tggtCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAAt                                                                                                                 >  1:103858/1‑140 (MQ=255)                      tggtCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAAt                                                                                                                 >  1:1232581/1‑140 (MQ=255)                        gtCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATgg                                                                                                               >  2:828390/1‑140 (MQ=255)                        gtCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATgg                                                                                                               <  1:732058/140‑1 (MQ=255)                         tCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGa                                                                                                              >  1:1061833/1‑140 (MQ=255)                                  gaATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGaaaa                                                                                                     >  2:99458/1‑140 (MQ=255)                                   aaTGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAAc                                                                                                    >  2:432470/1‑140 (MQ=255)                                   aaTGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAAc                                                                                                    >  2:1258264/1‑140 (MQ=255)                                   aaTGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAAc                                                                                                    >  1:933120/1‑140 (MQ=255)                                       ttCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGc                                                                                                          <  2:1126584/130‑1 (MQ=255)                                       ttCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGc                                                                                                          >  1:1126584/1‑130 (MQ=255)                                       ttCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGAc                                                                                                >  1:1436641/1‑140 (MQ=255)                                       ttCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGAc                                                                                                <  1:569680/140‑1 (MQ=255)                                        tCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACt                                                                                                   <  1:1316364/136‑1 (MQ=255)                                        tCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACt                                                                                                   >  2:1316364/1‑136 (MQ=255)                                         cACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCa                                                                                              >  1:1093246/1‑140 (MQ=255)                                           cGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGa                                                                                            <  2:933120/140‑1 (MQ=255)                                            ggTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGTTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGAc                                                                                           >  2:112159/1‑140 (MQ=255)                                            ggTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGAc                                                                                           <  1:432470/140‑1 (MQ=255)                                               gCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTc                                                                                        >  2:813977/1‑140 (MQ=255)                                                cAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCTCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTcc                                                                                       >  2:619823/1‑140 (MQ=255)                                                cAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTcc                                                                                       >  2:934162/1‑140 (MQ=255)                                                   ggggCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCAtt                                                                                    <  2:1093246/140‑1 (MQ=255)                                                   ggggCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCAtt                                                                                    <  2:1061833/140‑1 (MQ=255)                                                    gggCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTc                                                                                   <  2:1530647/140‑1 (MQ=255)                                                        aGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGa                                                                                            >  1:350258/1‑127 (MQ=255)                                                        aGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGa                                                                                            <  2:350258/127‑1 (MQ=255)                                                         gCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGTTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtg                                                                              <  1:112159/140‑1 (MQ=255)                                                         gCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtg                                                                              <  2:103858/140‑1 (MQ=255)                                                         gCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtg                                                                              <  1:99458/140‑1 (MQ=255)                                                         gCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtg                                                                              <  1:813977/140‑1 (MQ=255)                                                          ccTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtgg                                                                             >  1:585969/1‑140 (MQ=255)                                                          ccTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtgg                                                                             >  1:1357743/1‑140 (MQ=255)                                                          ccTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtgg                                                                             >  1:895825/1‑140 (MQ=255)                                                           cTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtggt                                                                            <  2:229773/140‑1 (MQ=255)                                                             tGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtggtgg                                                                          <  2:1232581/140‑1 (MQ=255)                                                             tGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtggtgg                                                                          >  1:774165/1‑140 (MQ=255)                                                              gAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTggtggt                                                                         >  2:270325/1‑140 (MQ=255)                                                              gAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTggtggt                                                                         <  2:1357743/140‑1 (MQ=255)                                                               aGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtg                                                                              <  1:788090/134‑1 (MQ=255)                                                               aGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtg                                                                              >  1:912177/1‑134 (MQ=255)                                                               aGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtg                                                                              >  2:369047/1‑134 (MQ=255)                                                               aGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtg                                                                              >  2:788090/1‑134 (MQ=255)                                                               aGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtg                                                                              <  2:912177/134‑1 (MQ=255)                                                               aGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtg                                                                              <  1:369047/134‑1 (MQ=255)                                                                   ggTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTtata                                                                    <  1:270325/140‑1 (MQ=255)                                                                    gTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtggt                                                                            <  1:1271767/131‑1 (MQ=255)                                                                    gTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtggt                                                                            >  2:1271767/1‑131 (MQ=255)                                                                    gTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtg                                                                              <  1:1263993/129‑1 (MQ=255)                                                                    gTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTtg                                                                              >  2:1263993/1‑129 (MQ=255)                                                                    gTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATAc                                                                   >  2:899227/1‑140 (MQ=255)                                                                    gTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATAc                                                                   >  2:1368723/1‑140 (MQ=255)                                                                    gTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATAc                                                                   >  1:898091/1‑140 (MQ=255)                                                                        gTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCg                                                               >  1:337982/1‑140 (MQ=255)                                                                               tATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCg                                                        <  1:934162/140‑1 (MQ=255)                                                                                   ctgcAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGc                                                    <  1:1258264/140‑1 (MQ=255)                                                                                   ctgcAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGc                                                    <  2:774165/140‑1 (MQ=255)                                                                                    tgcAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGcc                                                   <  2:787764/140‑1 (MQ=255)                                                                                    tgcAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGcc                                                   <  1:899227/140‑1 (MQ=255)                                                                                      cAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGtt                                                             <  2:1199962/128‑1 (MQ=255)                                                                                      cAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGtt                                                             >  1:1199962/1‑128 (MQ=255)                                                                                      cAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCcgt                                                 <  2:407911/140‑1 (MQ=255)                                                                                      cAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCcgt                                                 >  1:407911/1‑140 (MQ=255)                                                                                      cAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCcgt                                                 <  1:1134745/140‑1 (MQ=255)                                                                                        aCTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGcc                                                   >  2:1104016/1‑136 (MQ=255)                                                                                        aCTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGcc                                                   <  1:1104016/136‑1 (MQ=255)                                                                                        aCTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCcgtcg                                               >  2:691041/1‑140 (MQ=255)                                                                                            ctgctgcCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTaa                                           <  2:337982/140‑1 (MQ=255)                                                                                                   cGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATc                                    <  2:895825/140‑1 (MQ=255)                                                                                                       tgtgTTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGc                                <  1:116669/140‑1 (MQ=255)                                                                                                         tgtTGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCAt                              <  1:501149/140‑1 (MQ=255)                                                                                                           ttGGGGCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATaa                            >  2:913865/1‑140 (MQ=255)                                                                                                             ggggCATCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAg                          >  2:133196/1‑140 (MQ=255)                                                                                                                   tCTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCt                    >  2:716850/1‑140 (MQ=255)                                                                                                                    cTTTCTCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTg                   <  1:619823/140‑1 (MQ=255)                                                                                                                    cTTTCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTg                   <  1:1495046/140‑1 (MQ=255)                                                                                                                      ttCCCGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTggg                 <  1:473566/140‑1 (MQ=255)                                                                                                                        cccGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGa               >  1:1222145/1‑140 (MQ=255)                                                                                                                         ccGGCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGAt              <  2:585969/140‑1 (MQ=255)                                                                                                                            gCCGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCAt           >  2:58449/1‑140 (MQ=255)                                                                                                                             ccGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCAtt          <  1:58449/140‑1 (MQ=255)                                                                                                                              cGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTg                   <  2:286973/130‑1 (MQ=255)                                                                                                                              cGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTg                   >  1:286973/1‑130 (MQ=255)                                                                                                                              cGTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTg         <  2:119425/140‑1 (MQ=255)                                                                                                                               gTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCt                    >  1:269900/1‑128 (MQ=255)                                                                                                                               gTGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCt                    <  2:269900/128‑1 (MQ=255)                                                                                                                                tGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCTGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCt       <  2:898091/140‑1 (MQ=255)                                                                                                                                tGGATTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCt       <  1:133196/140‑1 (MQ=255)                                                                                                                                   aTTGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGtt    <  2:1436641/140‑1 (MQ=255)                                                                                                                                     tGGCGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATCGCTGTTTa  <  1:691041/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGCCTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTTTCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTA  >  NC_000913/2527368‑2527639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑