Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,527,823	T→C	V293A (GTG→GCG)	yfeH →	putative solute:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,527,823	0	T	C	100.0%	267.7 / NA	75	V293A (GTG→GCG)	yfeH	putative solute:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/41);  total (34/41)

CGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGAC  >  NC_000913/2527686‑2527951                                                                                                                                          |                                                                                                                                 cGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACCTTCTGTTCCCCACATCGGTGATCGGTATGATGgcgc                                                                                                                                >  1:1493521/1‑140 (MQ=255)  gTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGcgct                                                                                                                               <  2:371810/140‑1 (MQ=255)     tttATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGcc                                                                                                                            >  2:242270/1‑140 (MQ=255)     tttATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGcc                                                                                                                            >  2:154484/1‑140 (MQ=255)      ttATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGccc                                                                                                                           <  2:503816/140‑1 (MQ=255)      ttATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGccc                                                                                                                           <  2:1403476/140‑1 (MQ=255)      ttATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGccc                                                                                                                           <  2:170192/140‑1 (MQ=255)       tATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGcccc                                                                                                                          >  1:1624025/1‑140 (MQ=255)       tATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGcccc                                                                                                                          >  2:1119614/1‑140 (MQ=255)        aTGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCt                                                                                                                         <  1:20430/140‑1 (MQ=255)          ggCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCtga                                                                                                                       <  1:1119614/140‑1 (MQ=255)              cGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTgatgat                                                                                                                   >  1:679062/1‑140 (MQ=255)              cGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTgatgat                                                                                                                   >  1:1421893/1‑140 (MQ=255)              cGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTgatgat                                                                                                                   >  2:482082/1‑140 (MQ=255)                ccGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGcccc                                                                                                                          <  1:844573/131‑1 (MQ=255)                ccGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGcccc                                                                                                                          >  2:844573/1‑131 (MQ=255)                 cGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGACTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCGCATCGGTGATCGGGATGATGGCGCTGCCCCTGATGAtttt                                                                                                                >  2:1473065/1‑140 (MQ=255)                     tGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCac                                                                                                            <  1:1079448/140‑2 (MQ=255)                      gAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATc                                                                                                           <  1:1163243/140‑1 (MQ=255)                      gAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATc                                                                                                           <  2:772927/140‑1 (MQ=255)                            cAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATcc                                                                                                     <  1:1473065/140‑1 (MQ=255)                            cAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATcc                                                                                                     <  1:1352721/140‑1 (MQ=255)                                 aGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTg                                                                                                <  1:832133/140‑1 (MQ=255)                                   gCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGAt                                                                                              >  1:788900/1‑140 (MQ=255)                                   gCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGAt                                                                                              >  1:532915/1‑140 (MQ=255)                                      gATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGt                                                                                           <  1:450424/140‑1 (MQ=255)                                       aTGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATcc                                                                                                     <  1:425302/129‑1 (MQ=255)                                       aTGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATcc                                                                                                     >  2:425302/1‑129 (MQ=255)                                       aTGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGAt                                                                                              <  1:1142152/136‑1 (MQ=255)                                       aTGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGAt                                                                                              >  2:1142152/1‑136 (MQ=255)                                       aTGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTc                                                                                          >  1:790175/1‑140 (MQ=255)                                           aaTTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCtgtg                                                                                      <  1:482082/140‑1 (MQ=255)                                           aaTTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCtgtg                                                                                      <  2:1624025/140‑1 (MQ=255)                                           aaTTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCtgtg                                                                                      <  2:679062/140‑1 (MQ=255)                                           aaTTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCtgtg                                                                                      <  1:266607/140‑1 (MQ=255)                                            aTTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGc                                                                                     >  2:1095294/1‑140 (MQ=255)                                                  aTCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCt                                                                               >  2:314281/1‑140 (MQ=255)                                                    cGTCTTTTGTGGTTCGAAAAAGAGTCTGTCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTgg                                                                             <  1:478746/140‑1 (MQ=255)                                                     gTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGgc                                                                            >  1:331694/1‑140 (MQ=255)                                                      tCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGgcg                                                                           >  1:267923/1‑140 (MQ=255)                                                       cTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGgcgc                                                                          <  1:242270/140‑1 (MQ=255)                                                        ttttGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGcgcg                                                                         >  1:1658926/1‑140 (MQ=255)                                                           tgtgGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCg                                                                      >  2:830177/1‑140 (MQ=255)                                                           tgtgGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCg                                                                      <  2:131852/140‑1 (MQ=255)                                                             tgGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGAt                                                                    >  2:62278/1‑140 (MQ=255)                                                               gTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATAc                                                                  >  1:102938/1‑140 (MQ=255)                                                               gTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATAc                                                                  <  1:656863/140‑1 (MQ=255)                                                                ttCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACa                                                                 <  2:102938/140‑1 (MQ=255)                                                                         gagTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGa                                                        <  2:1421893/140‑1 (MQ=255)                                                                         gagTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGa                                                        <  2:331694/140‑1 (MQ=255)                                                                         gagTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTATGCGGTGCTGGCGCGTCGATACAAACGCCAGa                                                        <  2:842367/140‑1 (MQ=255)                                                                            tCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACa                                                                 >  2:1428425/1‑128 (MQ=255)                                                                            tCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACa                                                                 <  1:1428425/128‑1 (MQ=255)                                                                             cTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGa                                                    <  1:343642/140‑1 (MQ=255)                                                                                    aTGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGaa                                                   <  1:1625901/134‑1 (MQ=255)                                                                                    aTGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGaa                                                   >  2:1625901/1‑134 (MQ=255)                                                                                    aTGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTa                                             >  2:1507003/1‑140 (MQ=255)                                                                                        cATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAgg                                         <  2:551820/140‑1 (MQ=255)                                                                                        cATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAgg                                         <  2:1545582/140‑1 (MQ=255)                                                                                          tCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGgcg                                       >  2:927340/1‑140 (MQ=255)                                                                                          tCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGgcg                                       >  1:564819/1‑140 (MQ=255)                                                                                           cccGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGgcgc                                      <  2:788900/140‑1 (MQ=255)                                                                                                    aaaCATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGaaa                             <  1:314281/140‑1 (MQ=255)                                                                                                        aTTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAagcag                         <  2:1658926/140‑1 (MQ=255)                                                                                                            tGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGcagcag                                 <  1:560881/128‑1 (MQ=255)                                                                                                            tGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGcagcag                                 >  2:560881/1‑128 (MQ=255)                                                                                                             gTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCg                    >  1:1359181/1‑140 (MQ=255)                                                                                                              ttCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGa                   >  1:1209781/1‑140 (MQ=255)                                                                                                               tCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGAt                  <  1:927340/140‑1 (MQ=255)                                                                                                                        cGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAagc                           >  1:1078428/1‑122 (MQ=255)                                                                                                                        cGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAagc                           <  2:1078428/122‑1 (MQ=255)                                                                                                                          gTGATCGGTATGATGGCGCTGCCCCCGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAg       >  2:902716/1‑140 (MQ=255)                                                                                                                           tGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGc      >  1:1096666/1‑140 (MQ=255)                                                                                                                              tCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGa   <  1:1312971/140‑1 (MQ=255)                                                                                                                               cGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGAc  <  2:1493521/140‑1 (MQ=255)                                                                                                                                  tATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGa   >  1:1581914/1‑136 (MQ=255)                                                                                                                                  tATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGa   <  2:1581914/136‑1 (MQ=255)                                                                                                                                          |                                                                                                                                 CGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGAC  >  NC_000913/2527686‑2527951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑