Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,654,466	A→G	D156D (GAT→GAC)	sseB ←	protein SseB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,654,466	0	A	G	100.0%	267.9 / NA	75	D156D (GAT→GAC)	sseB	protein SseB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/37);  total (38/37)

GCCTGAATAATTTCTTCGATATCACCATCGGCTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGATTTCACGCGGCATAAATTCTTTA  >  NC_000913/2654329‑2654601                                                                                                                                          |                                                                                                                                        gCCTGAATAATTTCTTCGATATCACCATCGGCTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGagtc                                                                                                                                       >  2:504261/1‑140 (MQ=255)  ccTGAATAATTTCTTCGATATCACCATCGGCTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGagtca                                                                                                                                      <  2:263402/140‑1 (MQ=255)  ccTGAATAATTTCTTCGATATCACCATCGGCTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGagtca                                                                                                                                      <  1:504261/140‑1 (MQ=255)       ataatTTCTTCGATATCACCATCGGCTTTAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCAt                                                                                                                                 <  1:1053443/140‑1 (MQ=255)                      tCACCATCGGCTTCAATGCCAATAAGTAAATTAGGCTGTACCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGctc                                                                                                                  >  1:1206460/1‑140 (MQ=255)                       cACCATCGGCTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAAACATTTGTGCTGGCGGctct                                                                                                                 >  1:923071/1‑140 (MQ=255)                        aCCATCGGCTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTg                                                                                                                <  2:1549617/140‑1 (MQ=255)                         ccATCGGCTTCAATGCCAATAAGTAAATTAGGCTGTACCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGc                                                                                                               <  2:1206460/140‑1 (MQ=255)                           aTCGGCTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGa                                                                                                             <  2:1140181/140‑1 (MQ=255)                            tCGGCTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGAc                                                                                                            <  2:564432/140‑1 (MQ=255)                            tCGGCTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAAACATTTGTGCTGGCGGCTCTGCGAc                                                                                                            <  2:923071/140‑1 (MQ=255)                              ggCTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACtt                                                                                                          >  1:294167/1‑140 (MQ=255)                                cTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTcc                                                                                                        >  2:1389376/1‑140 (MQ=255)                                    aaTGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGata                                                                                                    >  2:1077754/1‑140 (MQ=255)                                      tGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGataat                                                                                                  <  2:468026/140‑1 (MQ=255)                                            tAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGc                                                                                            >  2:1615365/1‑140 (MQ=255)                                             aaGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCg                                                                                                       <  2:449551/128‑1 (MQ=255)                                             aaGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCg                                                                                                       >  1:449551/1‑128 (MQ=255)                                                   aTTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGTACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCAc                                                                                     >  1:741148/1‑140 (MQ=255)                                                   aTTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATAACAGCGATTCAc                                                                                     <  1:761847/140‑1 (MQ=255)                                                     tAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGata                                                                                                    >  2:1277521/1‑123 (MQ=255)                                                     tAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGata                                                                                                    <  1:1277521/123‑1 (MQ=255)                                                          tgtgCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTc                                                                              >  1:534348/1‑140 (MQ=255)                                                            tgCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCAc                                                                                     <  1:1600246/131‑1 (MQ=255)                                                            tgCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCAc                                                                                     >  2:1600246/1‑131 (MQ=255)                                                            tgCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCa                                                                            >  2:1636525/1‑140 (MQ=255)                                                            tgCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCa                                                                            >  1:1051948/1‑140 (MQ=255)                                                             gCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAg                                                                           >  2:728211/1‑140 (MQ=255)                                                              ccTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAgg                                                                          >  2:1595167/1‑140 (MQ=255)                                                               ctctTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGa                                                                         <  2:534348/140‑1 (MQ=255)                                                                tctTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGAt                                                                        <  2:294167/140‑1 (MQ=255)                                                                 ctTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCg                                                                                   >  2:1234159/1‑128 (MQ=255)                                                                 ctTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCg                                                                                   <  1:1234159/128‑1 (MQ=255)                                                                  ttCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGAttt                                                                      >  1:450535/1‑140 (MQ=255)                                                                  ttCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGAttt                                                                      >  2:761552/1‑140 (MQ=255)                                                                    cGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTcc                                                                    >  1:51774/1‑140 (MQ=255)                                                                      ttCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTg                                                                  >  2:1128808/1‑140 (MQ=255)                                                                      ttCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTg                                                                  >  1:455837/1‑140 (MQ=255)                                                                       tctcTTTAATTGAACAAATAAAAGTACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTgg                                                                 <  2:741148/140‑1 (MQ=255)                                                                           tttAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGgctgc                                                             >  2:926293/1‑140 (MQ=255)                                                                           tttAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGgctgc                                                             >  1:191098/1‑140 (MQ=255)                                                                            ttAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGgctgct                                                            >  1:684816/1‑140 (MQ=255)                                                                             tAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTc                                                           <  2:191098/140‑1 (MQ=255)                                                                                 tGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCg                                                       <  1:602167/140‑1 (MQ=255)                                                                                   aaCAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGa                                                     >  1:291615/1‑140 (MQ=255)                                                                                   aaCAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGa                                                     >  1:349368/1‑140 (MQ=255)                                                                                   aaCAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGa                                                     >  2:1596879/1‑140 (MQ=255)                                                                                     cAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGTGGAtt                                                   <  1:1077754/140‑1 (MQ=255)                                                                                     cAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGAtt                                                   >  2:1526923/1‑140 (MQ=255)                                                                                     cAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGAtt                                                   >  2:1664444/1‑140 (MQ=255)                                                                                      aaaTAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGAttt                                                  <  1:1084913/140‑1 (MQ=255)                                                                                      aaaTAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGAttt                                                  <  2:684816/140‑1 (MQ=255)                                                                                      aaaTAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGAttt                                                  <  1:1615365/140‑1 (MQ=255)                                                                                        aTAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTcc                                                <  2:1051948/140‑1 (MQ=255)                                                                                             aGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCtt                                           <  1:728211/140‑1 (MQ=255)                                                                                             aGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCtt                                           <  1:1389376/140‑1 (MQ=255)                                                                                                 cGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGAtt                                                   >  1:1460730/1‑128 (MQ=255)                                                                                                 cGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGAtt                                                   <  2:1460730/128‑1 (MQ=255)                                                                                                 cGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCAcc                                       >  2:317855/1‑140 (MQ=255)                                                                                                  gCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGAttt                                                  >  1:1122230/1‑128 (MQ=255)                                                                                                  gCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGAttt                                                  <  2:1122230/128‑1 (MQ=255)                                                                                                  gCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCa                                      >  2:331014/1‑140 (MQ=255)                                                                                                      cACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATca                                  <  1:1664444/140‑1 (MQ=255)                                                                                                      cACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATca                                  <  2:1290756/140‑1 (MQ=255)                                                                                                         cGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATcaaca                               <  1:1128808/140‑1 (MQ=255)                                                                                                           gCTTAATGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACaaa                             <  2:166950/140‑1 (MQ=255)                                                                                                                aTGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGAt                        >  2:498126/1‑140 (MQ=255)                                                                                                                aTGGTTTTAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGAt                        >  2:656126/1‑140 (MQ=255)                                                                                                                      ttAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGATTTCAcg                  >  2:216480/1‑140 (MQ=255)                                                                                                                      ttAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGATTTCAcg                  >  2:216118/1‑140 (MQ=255)                                                                                                                      ttAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGATTTCAcg                  >  2:1081391/1‑140 (MQ=255)                                                                                                                       tAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGATTTCAcgc                 <  2:455837/140‑1 (MQ=255)                                                                                                                       tAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGATTTCAcgc                 <  2:51774/140‑1 (MQ=255)                                                                                                                       tAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGATTTCAcgc                 <  1:331014/140‑1 (MQ=255)                                                                                                                       tAAATAAGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGATTTCAcgc                 <  1:498126/140‑1 (MQ=255)                                                                                                                            aaGGTGGTGAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGATTTCACGCGGCAt            <  1:216480/140‑1 (MQ=255)                                                                                                                               gtggtgAGTGAGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGATTTCACGCGGCATaaa         <  1:1526923/140‑1 (MQ=255)                                                                                                                                   tgagtgagTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGATTTCACGCGGCATAAATTCt     >  2:344906/1‑140 (MQ=255)                                                                                                                                   tgagtgagTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGATTTCACGCGGCATAAATTCt     >  2:819972/1‑140 (MQ=255)                                                                                                                                    gagtgagtCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGATTTCACGCGGCATAAATTCtt    <  1:1595167/140‑1 (MQ=255)                                                                                                                                      gtgaGTCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGATTTCACGCGGCATAAATTCTTTa  <  2:334085/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        GCCTGAATAATTTCTTCGATATCACCATCGGCTTCAATGCCAATAAGTAAATTAGGCTGTGCCTCTTCGTTCTCTTTAATTGAACAAATAAAAGCACGCTTCACCGGCTTAATGGTTTTAAATAAGGTGGTGAGTGAATCAATCATTTGTGCTGGCGGCTCTGCGACTTCCGATAATATCAGCGATTCACCGCCTTCCAGGATTTCCTGGCTGCTCAGCGGATTTCCCTCTTCACCAATCAACAAACTGATTTCACGCGGCATAAATTCTTTA  >  NC_000913/2654329‑2654601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑