Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	310	(T)9→8	intergenic (+55/‑27)	thrL → / → thrA	thr operon leader peptide/fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	302	0	T	.	100.0%	195.9 / NA	47	intergenic (+47/‑35)	thrL/thrA	thr operon leader peptide/fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (27/20);  total (27/20)

ATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGG  >  NC_000913/169‑436                                                                                                                                      |                                                                                                                                       aTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCttttttt                                                                                                                                  >  2:673940/1‑140 (MQ=255)        agTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCa                                                                                                                          <  2:693548/140‑1 (MQ=255)               aacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑‑TTTTTTTCGACCAAAGGTAAc                                                                                                                  <  2:198148/140‑1 (MQ=255)                acaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAAc                                                                                                                  <  1:283286/140‑1 (MQ=255)                  aTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGa                                                                                                                >  1:102807/1‑140 (MQ=255)                  aTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGa                                                                                                                >  2:82126/1‑140 (MQ=255)                    ccATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAgg                                                                                                              <  2:218122/140‑1 (MQ=255)                    ccATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAgg                                                                                                              >  2:90054/1‑140 (MQ=255)                    ccATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAgg                                                                                                              >  2:582742/1‑140 (MQ=255)                       tGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTaa                                                                                                           >  1:470364/1‑140 (MQ=255)                           aCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTaacaac                                                                                                       >  1:336540/1‑140 (MQ=255)                           aCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTaacaac                                                                                                       <  1:27934/140‑1 (MQ=255)                                   gCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAGACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAg                                                                                               >  2:591947/1‑140 (MQ=255)                                           aTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCACACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAg                                                                                       >  2:753134/1‑140 (MQ=255)                                             tACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGtt                                                                                     <  1:709060/140‑1 (MQ=255)                                             tACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGtt                                                                                     <  1:591947/140‑1 (MQ=255)                                              tcccccaccaTCCCCATTACCACAGGTAACGGTGCGGGCTGACGCGTTCAGGAAAACCAAAAAAAAGGCCGGCCCTGGCAGTGCGGGC‑GTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTc                                                                                    <  2:830272/136‑1 (MQ=255)                                                caccgccaGCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCGGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcgg                                                                                  <  1:814411/140‑1 (MQ=255)                                                caccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcgg                                                                                  <  2:102807/140‑1 (MQ=255)                                                   caccatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTTACAACCATGCGAGTGTTGAAGTTcggcgg                                                                               >  2:487191/1‑140 (MQ=255)                                                   caccatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggcgg                                                                               >  1:366385/1‑140 (MQ=255)                                                   caccatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggcgg                                                                               >  1:324604/1‑140 (MQ=255)                                                       atcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACa                                                                           >  1:145509/1‑140 (MQ=255)                                                       atcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACa                                                                           >  1:285263/1‑140 (MQ=255)                                                          accatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCa                                                                        <  1:582742/140‑1 (MQ=255)                                                               tACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGc                                                                   <  2:336540/140‑1 (MQ=255)                                                               tACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGc                                                                   <  2:406113/140‑1 (MQ=255)                                                                aCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCa                                                                  >  1:326627/1‑140 (MQ=255)                                                                    caGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATg                                                              >  1:376239/1‑140 (MQ=255)                                                                    caGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATg                                                              >  2:639228/1‑140 (MQ=255)                                                                      ggTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCa                                                            >  1:738325/1‑140 (MQ=255)                                                                              tGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGttt                                                    >  2:7305/1‑140 (MQ=255)                                                                                cGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTc                                                  >  2:503043/1‑140 (MQ=255)                                                                                 gggCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCt                                                 >  1:179582/1‑140 (MQ=255)                                                                                        cgcgTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGtt                                          >  1:453453/1‑140 (MQ=255)                                                                                          cgTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGc                                        <  1:82126/140‑1 (MQ=255)                                                                                            tACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCg                                      >  2:496578/1‑140 (MQ=255)                                                                                                         gAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAg                         <  2:285187/140‑1 (MQ=255)                                                                                                         gAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAg                         <  2:179582/140‑1 (MQ=255)                                                                                                          aaaaaaGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGc                        <  1:496578/140‑1 (MQ=255)                                                                                                          aaaaaaGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGc                        <  1:488590/140‑1 (MQ=255)                                                                                                               aGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGc                   <  1:639228/140‑1 (MQ=255)                                                                                                               aGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGc                   >  2:817653/1‑140 (MQ=255)                                                                                                                   cGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcagg               <  2:453453/140‑1 (MQ=255)                                                                                                                    gCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggc              >  2:432870/1‑140 (MQ=255)                                                                                                                            cAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggcag      <  1:7305/140‑1 (MQ=255)                                                                                                                               tGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTg   >  2:424888/1‑140 (MQ=255)                                                                                                                               tGCGGGC‑TTTTTTTTCGACAAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTg   >  1:674872/1‑140 (MQ=255)                                                                                                                                gCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTgg  >  1:781407/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       ATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGG  >  NC_000913/169‑436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑