Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,437,231	(T)9→8	intergenic (+2/+29)	micC → / ← ydbK	small regulatory RNA MicC/putative pyruvate‑flavodoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,437,223	0	T	.	100.0%	131.3 / NA	32	noncoding (103/109 nt)	micC	small regulatory RNA MicC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (12/20);  total (12/20)

AATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTG  >  NC_000913/1437095‑1437357                                                                                                                                 |                                                                                                                                       aaTTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCttttttttagaa                                                                                                                             <  2:189612/140‑5 (MQ=255) aaTTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCttttttttagaa                                                                                                                             <  1:646836/140‑5 (MQ=255)       cctttcGTTATTCTTGGCGGGTTAAATTGCTTTATTGGCCCAGAATTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGAt                                                                                                                      <  2:89898/135‑1 (MQ=255)       cTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGAt                                                                                                                      <  2:396436/140‑1 (MQ=255)       cTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGAt                                                                                                                      <  2:642638/140‑1 (MQ=255)            cGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATcc                                                                                                                 <  1:389518/140‑1 (MQ=255)                 ttCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATc                                                                                                            <  2:328789/140‑1 (MQ=255)                    tGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGAt                                                                                                                      <  2:712402/127‑1 (MQ=255)                    tGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGAt                                                                                                                      >  1:712402/1‑127 (MQ=255)                      cgcgGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGAt                                                                                                                      <  1:428133/125‑1 (MQ=255)                      cgcgGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGAt                                                                                                                      >  2:428133/1‑125 (MQ=255)                      cgcgGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCagag                                                                                                       >  2:285582/1‑140 (MQ=255)                      cgcgGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCagag                                                                                                       >  2:544442/1‑140 (MQ=255)                      cgcgGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCagag                                                                                                       >  1:345714/1‑140 (MQ=255)                       gcgGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCa                                                                                                          <  2:188794/136‑1 (MQ=255)                       gcgGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCa                                                                                                          >  1:188794/1‑136 (MQ=255)                           gTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGTC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCAttt                                                                                                  >  2:816355/1‑140 (MQ=255)                              atatGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAAt                                                                                               <  1:544442/140‑1 (MQ=255)                                     tttATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGtt                                                                                        >  1:1577/1‑140 (MQ=255)                                       tATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGc                                                                                      >  1:71121/1‑140 (MQ=255)                                           gTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCtttt                                                                                  <  1:117912/140‑1 (MQ=255)                                                             tGTTGAGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCAt                                                                <  1:687055/140‑1 (MQ=255)                                                                   gCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGc                                                          <  2:308515/140‑1 (MQ=255)                                                                     cattgcattgCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCa                                                        <  2:345714/140‑1 (MQ=255)                                                                             tgcCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCa                                                >  1:249323/1‑140 (MQ=255)                                                                                  cTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTAGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCg                                           >  2:782716/1‑140 (MQ=255)                                                                                             aCATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAAt                                >  2:464598/1‑140 (MQ=255)                                                                                                   aaaaaGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCTGGAAGTCATAGCGTTTTTGCAAATCAGCTg                          <  2:791177/140‑1 (MQ=255)                                                                                                         aCAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCAt                    <  2:395378/140‑1 (MQ=255)                                                                                                           aaGCCCGAACAGTAGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCt                  <  1:782716/140‑1 (MQ=255)                                                                                                              cccGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTc               <  2:718515/140‑1 (MQ=255)                                                                                                                 gAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCAt            <  2:249323/140‑1 (MQ=255)                                                                                                                       tcgtcCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGt      <  1:285582/140‑1 (MQ=255)                                                                                                                           ccGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTg  <  1:464598/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                       AATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTG  >  NC_000913/1437095‑1437357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑