Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,177,327	(A)7→6	intergenic (‑123/+185)	gatY ← / ← fbaB	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,177,321	0	A	.	100.0%	187.7 / NA	45	intergenic (‑117/+191)	gatY/fbaB	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (26/19);  total (26/19)

GCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCATCAGGCATAGGTTGCC  >  NC_000913/2177183‑2177453                                                                                                                                           |                                                                                                                                     gCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaa                                                                                                                                     >  1:761719/1‑140 (MQ=255)      gTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaac                                                                                                                                >  1:113098/1‑139 (MQ=255)         gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaacggc                                                                                                                             >  2:150986/1‑136 (MQ=255)            aCCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTc                                                                                                                         >  1:846294/1‑140 (MQ=255)                 gTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGAt                                                                                                                    <  2:846294/140‑1 (MQ=255)                 gTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGAt                                                                                                                    >  2:435169/1‑140 (MQ=255)                      ttttCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGa                                                                                                               <  2:49341/140‑1 (MQ=255)                                  gTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                                                                                   >  1:784712/1‑140 (MQ=255)                                  gTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                                                                                   >  2:744642/1‑140 (MQ=255)                                  gTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                                                                                   >  2:66145/1‑140 (MQ=255)                                   tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAg                                                                                                  >  1:708202/1‑140 (MQ=255)                                   tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAg                                                                                                  >  2:215863/1‑140 (MQ=255)                                   tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAg                                                                                                  >  1:500899/1‑140 (MQ=255)                                    cGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGt                                                                                                 >  1:209508/1‑140 (MQ=255)                                        tgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                                                                                             >  1:802240/1‑140 (MQ=255)                                                           taATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc                                                                          <  2:29702/140‑1 (MQ=255)                                                           taATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc                                                                          <  1:150986/140‑1 (MQ=255)                                                            aaTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                                                         <  1:66145/140‑1 (MQ=255)                                                            aaTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                                                         <  1:744642/140‑1 (MQ=255)                                                            aaTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                                                         >  2:283314/1‑140 (MQ=255)                                                              tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg                                                                           >  1:109770/1‑136 (MQ=255)                                                              tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg                                                                           <  2:109770/136‑1 (MQ=255)                                                                   ttattTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt                                                                       <  2:827453/135‑1 (MQ=255)                                                                   ttattTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt                                                                       >  1:827453/1‑135 (MQ=255)                                                                                aaTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGt                                                     <  2:463535/140‑1 (MQ=255)                                                                                 atatCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTg                                                    <  2:293185/140‑1 (MQ=255)                                                                                    tCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTa                                                 >  1:332855/1‑140 (MQ=255)                                                                                      gagaTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATg                                               <  1:215863/140‑1 (MQ=255)                                                                                      gagaTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATg                                               <  2:855278/140‑1 (MQ=255)                                                                                          tAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAgg                                           <  1:283314/140‑1 (MQ=255)                                                                                          tAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAgg                                           <  2:500899/140‑1 (MQ=255)                                                                                                   caAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAg                                  >  2:362213/1‑140 (MQ=255)                                                                                                    aaaaaCGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGa                                 <  1:835584/140‑1 (MQ=255)                                                                                                            cAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTCATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAg                         >  2:69746/1‑140 (MQ=255)                                                                                                              atatGAAAATTATTCGAGGAGTGAAAGGC‑GAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCg                       <  2:113098/140‑1 (MQ=255)                                                                                                                  gAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGc                   >  1:188996/1‑140 (MQ=255)                                                                                                                  gAAAATTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAACAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGc                   >  1:239529/1‑140 (MQ=255)                                                                                                                    aaaTTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCAt                 <  2:470771/140‑1 (MQ=255)                                                                                                                    aaaTTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCAt                 >  1:472667/1‑140 (MQ=255)                                                                                                                    aaaTTATTCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCAt                 >  1:32414/1‑140 (MQ=255)                                                                                                                       ttattCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCATCAg              >  1:447497/1‑140 (MQ=255)                                                                                                                         attCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCATCAGGc            >  1:697259/1‑140 (MQ=255)                                                                                                                         attCGAGGAGTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCATCAGGc            >  2:762097/1‑140 (MQ=255)                                                                                                                                  gTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCATCAGGCa           >  2:477684/1‑132 (MQ=255)                                                                                                                                  gTGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCATCAGGCa           <  1:477684/132‑1 (MQ=255)                                                                                                                                   tGAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCATCAGGCATAGGTTGcc  >  2:243482/1‑140 (MQ=255)                                                                                                                                    gAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCAt                 >  2:817682/1‑124 (MQ=255)                                                                                                                                    gAAAGGC‑AAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCAt                 <  1:817682/124‑1 (MQ=255)                                                                                                                                           |                                                                                                                                     GCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCATCAGGCATAGGTTGCC  >  NC_000913/2177183‑2177453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑