Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	550,467	(G)5→6	coding (29/894 nt)	ybcF →	putative carbamate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	550,462	1	.	G	100.0%	123.7 / NA	36	L8L (CTT→CTG)	ybcF	putative carbamate kinase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (17/19);  total (17/19)

GTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TT‑GGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTGGCACGCCTGGCCCGTTCTTATCGGTTGGCGATTGTTCACGGCAACGGGCC  >  NC_000913/550324‑550596                                                                                                                                             |                                                                                                                                       gTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATACGCTGCTGGGGTTCTGGCTTAGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TT‑g                                                                                                                                       <  2:563378/140‑1 (MQ=255)     cccGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TT‑ggggg                                                                                                                                   >  1:166149/1‑140 (MQ=255)         aaaCGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TT‑ggggggcaa                                                                                                                               <  2:371221/140‑5 (MQ=255)          aaCGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TT‑ggggggcaac                                                                                                                              >  2:714574/1‑135 (MQ=255)          aaCGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TT‑ggggggcaac                                                                                                                              >  1:119553/1‑135 (MQ=255)          aaCGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TT‑ggggggcaac                                                                                                                              >  1:367595/1‑135 (MQ=255)               aCCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCtt                                                                                                                          >  2:181731/1‑140 (MQ=255)                ccGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTa                                                                                                                         <  2:119553/140‑1 (MQ=255)                ccGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTa                                                                                                                         <  2:695376/140‑1 (MQ=255)                     gTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCa                                                                                                                    <  1:464191/140‑1 (MQ=255)                      ttGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTT‑GGGGTCAACGCCTTACTCCaa                                                                                                                   >  2:638473/1‑139 (MQ=255)                       tGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAgc                                                                                                                  <  1:181731/140‑1 (MQ=255)                        gCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAgcg                                                                                                                 >  1:502538/1‑140 (MQ=255)                                ttCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAgg                                                                                                         >  1:227558/1‑140 (MQ=255)                                               ggggCATACGTCAGGGGCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGaaa                                                                                          >  1:660801/1‑140 (MQ=255)                                                  gCATACGTCAGGGGCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATc                                                                                       <  2:579878/140‑1 (MQ=255)                                                          cAGGGGCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATcaatatcgc                                                                               <  2:367595/140‑1 (MQ=255)                                                             gggCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATcaatatcgcaat                                                                            <  2:18519/140‑1 (MQ=255)                                                                  gATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCaatatcgcaatatcgc                                                                       >  1:444727/1‑140 (MQ=255)                                                                  gATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCaatatcgcaatatcgc                                                                       <  1:638473/140‑1 (MQ=255)                                                                   aTACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATc                                                                                       <  1:488488/123‑1 (MQ=255)                                                                   aTACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATc                                                                                       >  2:488488/1‑123 (MQ=255)                                                                   aTACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGcc                                                                      <  2:502538/140‑1 (MQ=255)                                                                                  tCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCcgcg                                                       <  2:166149/140‑1 (MQ=255)                                                                                   cTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCgcgc                                                      >  2:186372/1‑140 (MQ=255)                                                                                     ggCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTg                                                    >  2:305055/1‑140 (MQ=255)                                                                                      gCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTgg                                                   <  2:660801/140‑1 (MQ=255)                                                                                      gCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTgg                                                   <  1:789642/140‑1 (MQ=255)                                                                                      gCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTgg                                                   >  1:638973/1‑140 (MQ=255)                                                                                        ttGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTGGCa                                                 >  2:811516/1‑140 (MQ=255)                                                                                           gCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTGGCACGc                                              >  1:198170/1‑140 (MQ=255)                                                                                               acaaTTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTGGCACGCCTgg                                          <  1:305055/140‑1 (MQ=255)                                                                                                caaTTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTGGCACGCCTGGc                                         >  2:88440/1‑140 (MQ=255)                                                                                                caaTTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTGGCACGCCTGGc                                         <  2:444727/140‑1 (MQ=255)                                                                                                caaTTATTACAAGGAAAACCATGAAAACACTGGTTGTGCCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTGGCACGCCTGGc                                         <  2:727002/140‑1 (MQ=255)                                                                                                             gAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTGGCACGCCTGGCCCGTTCTTATCgg                            >  2:501843/1‑140 (MQ=255)                                                                                                             gAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTGGCACGCCTGGCCCGTTCTTATCgg                            >  2:162274/1‑140 (MQ=255)                                                                                                             gAAAACCATGAAAACACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTGGCACGCCTGGCCCGTTCTTATCgg                            >  1:451664/1‑140 (MQ=255)                                                                                                                       aaaaCACTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTGGCACGCCTGGCCCGTTCTTATCGGTTGGCGATTg                  >  2:47635/1‑140 (MQ=255)                                                                                                                           cacTGGTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTGGCACGCCTGGCCCGTTCTTATCGGTTGGCGATTGTTCa              <  2:451664/140‑1 (MQ=255)                                                                                                                                gTTGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTGGCACGCCTGGCCCGTTCTTATCGGTTGGCGATTGTTCACGGCa         >  2:577737/1‑140 (MQ=255)                                                                                                                                 ttGTGGCTC‑TTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTGGCACGCCTGGCCCGTTCTTATCGGTTGGCGATTGTTCACGGCaa        <  1:88440/140‑1 (MQ=255)                                                                                                                                       ctc‑tTGGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTGGCACGCCTGGCCCGTTCTTATCGGTTGGCGATTGTTCACGGCAACAGGcc  <  1:714574/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                       GTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTC‑TT‑GGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTGGCACGCCTGGCCCGTTCTTATCGGTTGGCGATTGTTCACGGCAACGGGCC  >  NC_000913/550324‑550596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑