Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,750,728	(T)7→8	intergenic (‑20/‑103)	grpE ← / → nadK	nucleotide exchange factor GrpE/NAD kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,750,721	1	.	T	100.0%	155.6 / NA	45	intergenic (‑13/‑110)	grpE/nadK	nucleotide exchange factor GrpE/NAD kinase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (23/22);  total (23/22)

TTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCG‑‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTG  >  NC_000913/2750582‑2750855                                                                                                                                             |                                                                                                                                        tttCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCcgcg                                                                                                                                          >  2:10210/1‑140 (MQ=255)      tCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCG‑‑ttttt                                                                                                                                   >  2:709019/1‑140 (MQ=255)                   ccTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCAtc                                                                                                                       >  2:298938/1‑140 (MQ=255)                   ccTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCAtc                                                                                                                       >  1:855864/1‑140 (MQ=255)                        tCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCt                                                                                                                  >  2:742950/1‑140 (MQ=255)                        tCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCt                                                                                                                  >  1:632468/1‑140 (MQ=255)                           gcagAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAAc                                                                                                               <  1:10210/140‑1 (MQ=255)                              gAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTc                                                                                                            >  1:708717/1‑140 (MQ=255)                                 gCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCt                                                                                                         <  2:632468/140‑1 (MQ=255)                                      tGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCttatta                                                                                                    >  1:242326/1‑140 (MQ=255)                                           cAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATgggg                                                                                               <  1:756520/140‑1 (MQ=255)                                             aCTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCttatt                                                                                                     >  2:75989/1‑132 (MQ=255)                                             aCTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCttatt                                                                                                     <  1:75989/132‑1 (MQ=255)                                             aCTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGAt                                                                                             <  1:453506/140‑1 (MQ=255)                                             aCTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGAt                                                                                             <  2:679614/140‑1 (MQ=255)                                                              gTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCaa                                                                            >  1:147411/1‑140 (MQ=255)                                                                 cTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAggg                                                                         <  1:334208/140‑1 (MQ=255)                                                                 cTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAggg                                                                         >  1:130692/1‑140 (MQ=255)                                                                    aTCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGa                                                                        <  1:760680/138‑1 (MQ=255)                                                                    aTCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGa                                                                        >  2:760680/1‑138 (MQ=255)                                                                    aTCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAg                                                                      <  1:298938/140‑1 (MQ=255)                                                                        aTGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACt                                                                  <  2:855864/140‑1 (MQ=255)                                                                         tGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTc                                                                 <  2:792682/140‑1 (MQ=255)                                                                         tGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTc                                                                 <  2:147411/140‑1 (MQ=255)                                                                             aaTTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACAt                                                             <  1:709019/140‑1 (MQ=255)                                                                              aTTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAg                                                                      <  1:102450/130‑1 (MQ=255)                                                                              aTTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAg                                                                      >  2:102450/1‑130 (MQ=255)                                                                                 tcttcCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGt                                                          >  1:255759/1‑140 (MQ=255)                                                                                    tcCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGtcatc                                                      >  2:791988/1‑140 (MQ=255)                                                                                            ttGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCg                                              <  2:1006/140‑1 (MQ=255)                                                                                                       gCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGAcc                                   <  1:88770/140‑1 (MQ=255)                                                                                                       gCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGAcc                                   >  1:814992/1‑140 (MQ=255)                                                                                                          ttttCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCg                                <  2:242326/140‑1 (MQ=255)                                                                                                          ttttCTGTTCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCg                                >  1:433625/1‑140 (MQ=255)                                                                                                                 ttCTTTACTACTCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAAt                         >  2:701635/1‑140 (MQ=255)                                                                                                                        ctactCATGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGaataat                  >  1:12965/1‑140 (MQ=255)                                                                                                                         tactCATGAATTTCTCCGCGTTTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGaataat                  <  2:255759/140‑1 (MQ=255)                                                                                                                              aTGAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTc            <  2:465162/140‑1 (MQ=255)                                                                                                                                gAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCaa          >  1:118019/1‑140 (MQ=255)                                                                                                                                gAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCaa          <  2:814992/140‑1 (MQ=255)                                                                                                                                gAATTTCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCaa          <  2:708717/140‑1 (MQ=255)                                                                                                                                   tttCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAgtg       >  2:1680/1‑140 (MQ=255)                                                                                                                                   tttCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAg         <  1:81188/138‑1 (MQ=255)                                                                                                                                   tttCTCCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAg         >  2:81188/1‑138 (MQ=255)                                                                                                                                     tctcCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTa     >  2:540697/1‑140 (MQ=255)                                                                                                                                      ctcCGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTAt    >  2:283650/1‑140 (MQ=255)                                                                                                                                        ccGCGT‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTg  >  1:823891/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                        TTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCG‑‑TTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTG  >  NC_000913/2750582‑2750855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑