Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,398,571	T→C	Q102R (CAG→CGG)	mreD ←	cell shape determining protein MreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,398,571	0	T	C	100.0%	339.4 / NA	95	Q102R (CAG→CGG)	mreD	cell shape determining protein MreD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (56/39);  total (56/39)

CCACGGCCAGAGCACCCCATTGACTACACTACTCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCTGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCAGTATGGCACCCATCACAAAACCT  >  NC_000913/3398435‑3398702                                                                                                                                         |                                                                                                                                    ccACGGCCAGAGCACCCCATTGACTACACTACTCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTgccgcc                                                                                                                                  <  2:853339/140‑1 (MQ=255)  cACGGCCAGAGCACCCCATTGACTACACTACTCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCa                                                                                                                                 >  1:437556/1‑140 (MQ=255)  cACGGCCAGAGCACCCCATTGACTACACTACTCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCa                                                                                                                                 <  1:366197/140‑1 (MQ=255)           agCACCCCATTGACTACACTACTCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAg                                                                                                                        >  2:353629/1‑140 (MQ=255)                cccATTGACTACACTACTCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGc                                                                                                                   >  1:573225/1‑140 (MQ=255)                 ccATTGACTACACTACTCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCg                                                                                                                  >  1:175002/1‑140 (MQ=255)                     tGACTACACTACTCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGaaa                                                                                                              >  1:506991/1‑140 (MQ=255)                     tGACTACACTACTCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGaaa                                                                                                              >  1:742803/1‑140 (MQ=255)                       actacactacTCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAg                                                                                                            >  2:321938/1‑140 (MQ=255)                        ctacactacTCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGc                                                                                                           <  1:297693/140‑1 (MQ=255)                         tacactacTCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCt                                                                                                          >  2:813680/1‑140 (MQ=255)                             ctactCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTa                                                                                                      >  2:709055/1‑140 (MQ=255)                              tactCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTAt                                                                                                     >  2:692107/1‑140 (MQ=255)                              tactCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAACGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTAt                                                                                                     >  1:406091/1‑140 (MQ=255)                               actCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTAtt                                                                                                    <  1:685909/140‑1 (MQ=255)                                ctCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTAttt                                                                                                   >  1:70944/1‑140 (MQ=255)                                ctCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTAttt                                                                                                   >  1:146356/1‑140 (MQ=255)                                ctCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTAttt                                                                                                   >  2:388570/1‑140 (MQ=255)                                 tCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTc                                                                                                  >  1:314307/1‑140 (MQ=255)                                    aGAACACTCCCGTTCTGAAAAAGACGTAAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAgc                                                                                               <  2:506991/140‑1 (MQ=255)                                     gAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAgcg                                                                                              >  1:104996/1‑140 (MQ=255)                                     aaaCACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAgcg                                                                                              <  2:588757/139‑1 (MQ=255)                                      aaCACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAgcgc                                                                                             >  2:7775/1‑140 (MQ=255)                                      aaCACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAgcgc                                                                                             >  1:56193/1‑140 (MQ=255)                                       acacTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGcc                                                                                            >  1:861640/1‑140 (MQ=255)                                       acacTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGcc                                                                                            <  1:252488/140‑1 (MQ=255)                                         agttCCGGTCTGAGAGCGACGTTAGTCATTAAAAACGCTGCCCAGAAAACAATCATATCCCCCACCAGCGAAAGCAGCAGGGCGCCCTGCGCCCGCTGCCATAATGCGGGGTTGCGGAAAAGCTGGTATTTGAGCGccac                                                                                          <  2:811230/138‑1 (MQ=255)                                          cTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGccacc                                                                                         >  2:291478/1‑140 (MQ=255)                                           ttCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGccacca                                                                                        >  2:102875/1‑140 (MQ=255)                                            tCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAg                                                                                       <  2:573225/140‑1 (MQ=255)                                             ccGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGccac                                                                                          >  2:828583/1‑136 (MQ=255)                                             ccGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGccac                                                                                          <  1:828583/136‑1 (MQ=255)                                             ccGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAgg                                                                                      >  2:338507/1‑140 (MQ=255)                                               ggTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTa                                                                                    <  2:233188/140‑1 (MQ=255)                                                 tCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAg                                                                                  <  1:399708/140‑1 (MQ=255)                                                 tCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAg                                                                                  <  2:423925/140‑1 (MQ=255)                                                 tCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAg                                                                                  <  1:388570/140‑1 (MQ=255)                                                     aaaGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAat                                                                              >  2:586644/1‑140 (MQ=255)                                                     aaaGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAat                                                                              >  2:454531/1‑140 (MQ=255)                                                     aaaGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAat                                                                              >  2:346093/1‑140 (MQ=255)                                                     aaaGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAat                                                                              >  1:243618/1‑140 (MQ=255)                                                     aaaGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAat                                                                              >  2:65667/1‑140 (MQ=255)                                                     aaaGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAat                                                                              >  2:676952/1‑140 (MQ=255)                                                       agagACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAatga                                                                            <  1:338507/140‑1 (MQ=255)                                                           aCGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCt                                                                        >  1:704519/1‑140 (MQ=255)                                                           aCGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACAGGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCt                                                                        <  2:56193/140‑1 (MQ=255)                                                               tAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATc                                                                    >  2:190780/1‑140 (MQ=255)                                                                aaTCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCg                                                                   >  1:417549/1‑140 (MQ=255)                                                                    aCTAAAACCTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCaa                                                               <  1:773391/140‑1 (MQ=255)                                                                       aaaaaCTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATAc                                                            >  1:236033/1‑140 (MQ=255)                                                                        aaaaCTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATAcg                                                           >  1:313194/1‑140 (MQ=255)                                                                            ctctGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTa                                                       >  2:271344/1‑140 (MQ=255)                                                                               tGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACACGTACGc                                                    >  1:7736/1‑140 (MQ=255)                                                                                gCCCAGAAAACAATAATATCCACCATCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGcc                                                   >  1:700895/1‑140 (MQ=255)                                                                                gCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAAt                                                              <  1:513819/129‑1 (MQ=255)                                                                                gCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAAt                                                              >  2:513819/1‑129 (MQ=255)                                                                                gCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAAt                                                              >  2:339032/1‑129 (MQ=255)                                                                                gCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAAt                                                              <  1:339032/129‑1 (MQ=255)                                                                                gCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGcc                                                   >  1:249389/1‑140 (MQ=255)                                                                                              aataTCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGc                                     <  1:65667/140‑1 (MQ=255)                                                                                              aataTCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGc                                     <  1:586644/140‑1 (MQ=255)                                                                                               atatCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCt                                    <  1:190780/140‑1 (MQ=255)                                                                                                tatCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTg                                   <  1:51885/140‑1 (MQ=255)                                                                                                tatCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTg                                   >  1:660927/1‑140 (MQ=255)                                                                                                  tCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGAt                                 <  1:346093/140‑1 (MQ=255)                                                                                                  tCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGAt                                 <  1:361850/140‑1 (MQ=255)                                                                                                  tCCACCACCAGCGAAAGCAACAAGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGAt                                 <  1:169240/140‑1 (MQ=255)                                                                                                  ccccccaccaGCGAAAGCAACATGACGCCCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGAt                                 <  2:417549/136‑1 (MQ=255)                                                                                                     accaccaGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTTGAGCCGCTGATCAg                              >  1:804973/1‑140 (MQ=255)                                                                                                     accaccaGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAg                              >  1:577051/1‑140 (MQ=255)                                                                                                      ccaccaGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGAt                                 <  1:702856/136‑1 (MQ=255)                                                                                                      ccaccaGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGAt                                 >  2:702856/1‑136 (MQ=255)                                                                                                       caccaGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGAt                            >  2:691572/1‑140 (MQ=255)                                                                                                        accaGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATc                           <  1:692107/140‑1 (MQ=255)                                                                                                        accaGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATc                           <  1:353629/140‑1 (MQ=255)                                                                                                         ccaGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATcc                          <  1:813680/140‑1 (MQ=255)                                                                                                          caGCGATAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCa                         >  1:837183/1‑140 (MQ=255)                                                                                                          caGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTTGAGCCGCTGATCAGATCCa                         <  2:804973/140‑1 (MQ=255)                                                                                                          caGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGAt                                 >  2:92307/1‑132 (MQ=255)                                                                                                          caGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGAt                                 <  1:92307/132‑1 (MQ=255)                                                                                                          caGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCa                         >  1:232455/1‑140 (MQ=255)                                                                                                           aGCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCAg                        >  2:283133/1‑140 (MQ=255)                                                                                                            gCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGc                                     >  2:800516/1‑126 (MQ=255)                                                                                                            gCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGc                                     <  1:800516/126‑1 (MQ=255)                                                                                                            gCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCa                               >  1:762219/1‑132 (MQ=255)                                                                                                            gCGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCa                               <  2:762219/132‑1 (MQ=255)                                                                                                             caaaaCCAACATCACGACCACCCCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCAGTa                      <  2:313194/138‑1 (MQ=255)                                                                                                             cGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCAGTa                      >  1:164655/1‑140 (MQ=255)                                                                                                             cGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCAGTa                      >  2:265665/1‑140 (MQ=255)                                                                                                             cGAAAGCAACATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCAGTa                      >  2:324692/1‑140 (MQ=255)                                                                                                                     aCATGACGACCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCAGTATGGCAccc              <  1:691572/140‑1 (MQ=255)                                                                                                                           cgacCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCAGTATGGCACCCATcaca        <  1:709055/140‑1 (MQ=255)                                                                                                                             acCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCAGTATGACAcc               >  2:701790/1‑131 (MQ=255)                                                                                                                             acCAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCAGTATGACAcc               <  1:701790/131‑1 (MQ=255)                                                                                                                              ccAGCGCCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCAGTATGGCACCCATCACaaaa     >  1:32639/1‑140 (MQ=255)                                                                                                                                 gcgcCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCAGTATGGCACCCATCACAAAACCt  <  2:236033/140‑1 (MQ=255)                                                                                                                                 gcgcCTGCCGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCAGTATGGCACCCATCACAAAACCt  <  2:577051/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    CCACGGCCAGAGCACCCCATTGACTACACTACTCCAGAACACTTCCGGTCTGAAAGAGACGTTAATCACTAAAAACTCTGCCCAGAAAACAATAATATCCACCACCAGCGAAAGCAACATGACGACCAGCGCCTGCTGCCATAATGCGAGGTTGCGGAAAAGCTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCAGTATGGCACCCATCACAAAACCT  >  NC_000913/3398435‑3398702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑