Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,434,765	G→A	L184L (TTG→TTA)	fmt →	10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,434,765	0	G	A	100.0%	197.8 / NA	57	L184L (TTG→TTA)	fmt	10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/27);  total (30/27)

TATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCG  >  NC_000913/3434630‑3434901                                                                                                                                        |                                                                                                                                         tATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAaac                                                                                                                                      >  2:426878/1‑140 (MQ=255)  aTGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAaaca                                                                                                                                     >  1:588124/1‑140 (MQ=255)         tGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAaac                                                                                                                                      >  2:412784/1‑132 (MQ=255)         tGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAaac                                                                                                                                      <  1:412784/132‑1 (MQ=255)           gATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGa                                                                                                                            >  1:83649/1‑140 (MQ=255)           gATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGa                                                                                                                            >  1:650153/1‑140 (MQ=255)             tGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGacg                                                                                                                          >  2:764159/1‑140 (MQ=255)             tGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGacg                                                                                                                          >  1:66565/1‑140 (MQ=255)             tGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGacg                                                                                                                          >  1:461037/1‑140 (MQ=255)                    ttAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCGGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGacggcacggc                                                                                                                   >  1:417136/1‑140 (MQ=255)                    ttAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGacggcacggc                                                                                                                   <  2:600888/140‑1 (MQ=255)                    ttAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGacggcacggc                                                                                                                   <  2:588124/140‑1 (MQ=255)                           ccGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCa                                                                                                            <  2:262764/140‑1 (MQ=255)                            cGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAg                                                                                                           <  2:461037/140‑1 (MQ=255)                               tGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAg                                                                                                        >  2:107085/1‑140 (MQ=255)                                 atatGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGtt                                                                                                      >  1:172751/1‑140 (MQ=255)                                  tatGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTc                                                                                                     >  1:728915/1‑140 (MQ=255)                                         tataAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGa                                                                                              <  1:426878/140‑1 (MQ=255)                                           taAGCTCTCCTGCCCGATTACTGCAGAAGATACCGGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGaaa                                                                                            <  2:417136/140‑1 (MQ=255)                                           taAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGaaa                                                                                            >  2:244586/1‑140 (MQ=255)                                            aaGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAAc                                                                                           <  1:737549/140‑1 (MQ=255)                                                tcgccTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACAACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCtt                                                                                       <  1:685049/137‑1 (MQ=255)                                                   ccTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCTAAACCAGAAGTTCAGGACGAAACTCTTGTc                                                                                    >  1:498798/1‑140 (MQ=255)                                                   ccTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTc                                                                                    >  1:622132/1‑140 (MQ=255)                                                   ccTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTc                                                                                    >  2:557066/1‑140 (MQ=255)                                                   ccTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTc                                                                                    >  1:802598/1‑140 (MQ=255)                                                              aCTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCga                                                                         >  2:568301/1‑140 (MQ=255)                                                               cTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCgag                                                                        <  2:622132/140‑1 (MQ=255)                                                               cTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCgag                                                                        <  2:631147/140‑1 (MQ=255)                                                               cTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCgag                                                                        <  1:107085/140‑1 (MQ=255)                                                                  cAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAg                                                                     <  2:525824/140‑1 (MQ=255)                                                                         tACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTa                                                              <  1:244586/140‑1 (MQ=255)                                                                            cAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAaag                                                           >  2:294400/1‑140 (MQ=255)                                                                                  tACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAaagaagaag                                                     <  2:802598/140‑1 (MQ=255)                                                                                  tACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAaagaagaag                                                     <  2:675337/140‑1 (MQ=255)                                                                                  tACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAaagaagaag                                                     >  1:675337/1‑140 (MQ=255)                                                                                      cTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAgc                                                    <  2:742287/137‑1 (MQ=255)                                                                                      cTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAgc                                                    >  1:742287/1‑137 (MQ=255)                                                                                         tACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTAt                                              >  2:470555/1‑140 (MQ=255)                                                                                            gACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGa                                           <  2:268927/140‑1 (MQ=255)                                                                                            gACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGa                                           <  2:66565/140‑1 (MQ=255)                                                                                                 gCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAaagaagaag                                                     >  2:336948/1‑125 (MQ=255)                                                                                                 gCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAaagaagaag                                                     <  1:336948/125‑1 (MQ=255)                                                                                                 gCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGt                                      >  1:864733/1‑140 (MQ=255)                                                                                                   tGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCa                                    >  1:217049/1‑140 (MQ=255)                                                                                                     gCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACt                                  <  1:529944/140‑1 (MQ=255)                                                                                                           cTTGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGgc                            >  1:221385/1‑140 (MQ=255)                                                                                                            ttGGCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGgca                           >  1:548343/1‑140 (MQ=255)                                                                                                              ggCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGgcagc                         <  2:172751/140‑1 (MQ=255)                                                                                                               gCCCACAAGGGCTTATCACCACGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGgcagca                        <  1:764159/140‑1 (MQ=255)                                                                                                                            tATCACCACGTTAAAACAACTGGCAGACGGCACGGCTAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCt           <  2:498798/140‑1 (MQ=255)                                                                                                                                  cacGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTc     >  1:573240/1‑140 (MQ=255)                                                                                                                                   acGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAATGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTcg    >  1:632268/1‑140 (MQ=255)                                                                                                                                   acGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTcg    <  2:573240/140‑1 (MQ=255)                                                                                                                                   acGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTcg    >  1:557191/1‑140 (MQ=255)                                                                                                                                    cGTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTcgc   <  2:221385/140‑1 (MQ=255)                                                                                                                                     gTTAAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTcgcg  <  2:557191/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCAGTGGTACGCTGTACGACAAGCTGGCAGAGCTTGGCCCACAAGGGCTTATCACCACGTTGAAACAACTGGCAGACGGCACGGCGAAACCAGAAGTTCAGGACGAAACTCTTGTCACTTACGCCGAGAAGTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCG  >  NC_000913/3434630‑3434901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑