Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,501,803	A→T	D179V (GAT→GTT)	frlB →	fructoselysine 6‑phosphate deglycase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,501,803	0	A	T	100.0%	188.3 / NA	55	D179V (GAT→GTT)	frlB	fructoselysine 6‑phosphate deglycase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (29/26);  total (29/26)

CGGCGGAATTTAGCATTGATTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGATCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCGGGTCCGCTGCGTCCGCTGGG  >  NC_000913/3501674‑3501935                                                                                                                                  |                                                                                                                                     cggcggAATTTAGCATTGATTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAg                                                                                                                            >  1:694119/1‑140 (MQ=255)     ggAATTTAGCATTGATTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGc                                                                                                                        <  2:602516/140‑1 (MQ=255)     ggAATTTAGCATTGATTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGc                                                                                                                        <  2:257881/140‑1 (MQ=255)              cATTGATTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGa                                                                                                                     <  2:779633/134‑1 (MQ=255)              cATTGATTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGa                                                                                                                     >  1:779633/1‑134 (MQ=255)               aTTGATTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCt                                                                                                              <  2:583620/140‑1 (MQ=255)                 tGATTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCg                                                                                                            >  1:156964/1‑140 (MQ=255)                   aTTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCgg                                                                                                           >  2:23937/1‑139 (MQ=255)                   aTTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCgg                                                                                                           <  1:23937/139‑1 (MQ=255)                    ttATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTc                                                                                                         <  2:35592/140‑1 (MQ=255)                           gCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGt                                                                                                  >  1:365378/1‑140 (MQ=255)                            ccGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTa                                                                                                 <  1:41975/140‑1 (MQ=255)                               aCTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGc                                                                                              >  1:99656/1‑140 (MQ=255)                                    aTCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTg                                                                                         <  2:365378/140‑1 (MQ=255)                                             aTTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGaaaa                                                                                >  1:779908/1‑140 (MQ=255)                                             aTTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGaaaa                                                                                >  1:252697/1‑140 (MQ=255)                                                    tgctgcTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGgccgc                                                                         <  2:99656/140‑1 (MQ=255)                                                    tgctgcTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGgccgc                                                                         <  1:570831/140‑1 (MQ=255)                                                         ctctgctACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCt                                                                    >  1:258075/1‑140 (MQ=255)                                                         ctctgctACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCt                                                                    >  2:23448/1‑140 (MQ=255)                                                                 cAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTg                                                                  <  2:253515/134‑1 (MQ=255)                                                                 cAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTg                                                                  >  1:253515/1‑134 (MQ=255)                                                                   gCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTg                                                          >  2:261283/1‑140 (MQ=255)                                                                      tggtgCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGgcc                                                       >  2:449840/1‑140 (MQ=255)                                                                      tggtgCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGgcc                                                       >  2:584708/1‑140 (MQ=255)                                                                        gtgCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGgccag                                                     >  2:250238/1‑140 (MQ=255)                                                                           cTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGgccagcca                                                  <  2:779908/140‑1 (MQ=255)                                                                            tGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGccagccag                                                 >  2:760307/1‑140 (MQ=255)                                                                              gagaTGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTg                                               >  2:59476/1‑140 (MQ=255)                                                                                  tgatCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAGGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCg                                           <  2:694119/140‑1 (MQ=255)                                                                                   gatCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCga                                          <  2:156964/140‑1 (MQ=255)                                                                                   gatCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCga                                          >  2:221497/1‑140 (MQ=255)                                                                                   gatCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCga                                          >  1:272849/1‑140 (MQ=255)                                                                                   gatCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCga                                          >  2:428262/1‑140 (MQ=255)                                                                                   gatCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCg                                           <  2:642678/139‑1 (MQ=255)                                                                                   gatCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCg                                           >  1:642678/1‑139 (MQ=255)                                                                                        cccGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGAtt                                     <  1:760307/140‑1 (MQ=255)                                                                                          cGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTta                                   >  2:780384/1‑140 (MQ=255)                                                                                           gCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTtat                                  >  2:288957/1‑140 (MQ=255)                                                                                              tCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATAcc                               <  2:275571/140‑1 (MQ=255)                                                                                              tCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATAcc                               >  2:601902/1‑140 (MQ=255)                                                                                              tCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATAcc                               >  2:668013/1‑140 (MQ=255)                                                                                                 cgcCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGtt                            >  2:779741/1‑140 (MQ=255)                                                                                                   ccGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTtgc                          <  2:542971/140‑1 (MQ=255)                                                                                                   ccGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTtgc                          <  2:825337/140‑1 (MQ=255)                                                                                                       aCGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCg                      >  1:165037/1‑140 (MQ=255)                                                                                                       aCGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCg                      >  1:467607/1‑140 (MQ=255)                                                                                                        cgcgGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCgg                     <  1:59476/140‑1 (MQ=255)                                                                                                          cgGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCGGGt                   >  1:216721/1‑140 (MQ=255)                                                                                                                      aGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCGGGTCCGCTGCGTCCg       <  1:288957/140‑1 (MQ=255)                                                                                                                      aGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCGGGTCCGCTGCGTCCg       <  1:710715/140‑1 (MQ=255)                                                                                                                      aGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCGGGTCCGCTGCGTCCg       <  2:252697/140‑1 (MQ=255)                                                                                                                        aTCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCGGGTCCGCTGCGTCCGCt     <  1:23448/140‑1 (MQ=255)                                                                                                                        aTCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCGGGTCCGCTGCGTCCGCt     <  2:272849/140‑1 (MQ=255)                                                                                                                           aaaaaCGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCGGGTCCGCTGCGTCCGCTggg  <  1:668013/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                     CGGCGGAATTTAGCATTGATTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGATCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCGGGTCCGCTGCGTCCGCTGGG  >  NC_000913/3501674‑3501935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑