Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,546,044	G→A	A141T (GCC→ACC)	nfuA →	iron‑sulfur cluster carrier protein NfuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,546,044	0	G	A	100.0%	237.0 / NA	68	A141T (GCC→ACC)	nfuA	iron‑sulfur cluster carrier protein NfuA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/36);  total (32/36)

CGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCG  >  NC_000913/3545908‑3546179                                                                                                                                         |                                                                                                                                        cGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCa                                                                                                                                      <  2:26039/140‑1 (MQ=255) cGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCa                                                                                                                                      <  1:560786/140‑1 (MQ=255)    cAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTc                                                                                                                                   <  1:628422/140‑1 (MQ=255)               aaGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTggc                                                                                                                        <  1:708614/140‑1 (MQ=255)                aGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTggcg                                                                                                                       >  2:680903/1‑140 (MQ=255)                 gTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTggcgg                                                                                                                      <  2:68027/140‑1 (MQ=255)                  tGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTggcggc                                                                                                                     <  1:824372/140‑1 (MQ=255)                   ggCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTggcggcg                                                                                                                    <  1:437882/140‑1 (MQ=255)                          gATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCCGTaa                                                                                                             >  2:88728/1‑140 (MQ=255)                          gATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGATCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTaa                                                                                                             <  2:404207/140‑1 (MQ=255)                              cACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGgt                                                                                                         >  2:510825/1‑140 (MQ=255)                               aCCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGgtt                                                                                                        <  1:680903/140‑1 (MQ=255)                                    tGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTcc                                                                                                   <  1:261132/140‑1 (MQ=255)                                         gAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGt                                                                                              <  2:865695/140‑1 (MQ=255)                                         gAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGt                                                                                              <  1:230341/140‑1 (MQ=255)                                              cgTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGAtg                                                                                         >  2:457680/1‑140 (MQ=255)                                                       tatGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGa                                                                                <  1:668576/140‑1 (MQ=255)                                                             gcAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAaagaag                                                                          >  1:23556/1‑140 (MQ=255)                                                             gcAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAaagaag                                                                          >  1:636901/1‑140 (MQ=255)                                                             gcAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAaagaag                                                                          >  2:811738/1‑140 (MQ=255)                                                             gcAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAaagaa                                                                           >  2:574941/1‑139 (MQ=255)                                                             gcAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAaagaa                                                                           <  1:574941/139‑1 (MQ=255)                                                              cAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAgg                                                                         <  2:23556/140‑1 (MQ=255)                                                              cAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAgg                                                                         >  2:623940/1‑140 (MQ=255)                                                               aGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAggg                                                                        >  1:466423/1‑140 (MQ=255)                                                                 tCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAaagaag                                                                          >  2:100527/1‑136 (MQ=255)                                                                 tCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAaagaag                                                                          <  1:100527/136‑1 (MQ=255)                                                                 tCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGAt                                                                      <  2:617405/140‑1 (MQ=255)                                                                 tCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGAt                                                                      >  2:231214/1‑140 (MQ=255)                                                                   gCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCg                                                                    >  2:36846/1‑140 (MQ=255)                                                                           aCCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGAt                                                                      >  2:99841/1‑130 (MQ=255)                                                                           aCCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGAt                                                                      <  1:99841/130‑1 (MQ=255)                                                                           aCCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAagcag                                                            <  1:36846/140‑1 (MQ=255)                                                                           aCCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAagcag                                                            >  1:583179/1‑140 (MQ=255)                                                                                 aGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGctgctg                                                      <  1:510825/140‑1 (MQ=255)                                                                                 aGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGctgctg                                                      >  2:670813/1‑140 (MQ=255)                                                                                   cTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGaa                                                    >  1:534335/1‑140 (MQ=255)                                                                                      gCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGa                                                 >  2:658071/1‑140 (MQ=255)                                                                                      gCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGa                                                 >  1:142453/1‑140 (MQ=255)                                                                                      gCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGa                                                 >  2:576201/1‑140 (MQ=255)                                                                                           tCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTcc                                            <  1:627098/140‑1 (MQ=255)                                                                                            cACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTccc                                           >  2:437739/1‑140 (MQ=255)                                                                                             aCGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTcc                                            <  2:264146/138‑1 (MQ=255)                                                                                             aCGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTcc                                            >  1:264146/1‑138 (MQ=255)                                                                                             aCGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCg                                          <  1:623940/140‑1 (MQ=255)                                                                                             aCGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCg                                          >  1:450001/1‑140 (MQ=255)                                                                                             aCGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCg                                          <  2:583179/140‑1 (MQ=255)                                                                                             aCGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCg                                          <  1:231214/140‑1 (MQ=255)                                                                                             aCGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCCGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCg                                          <  1:88728/140‑1 (MQ=255)                                                                                               ggtggtCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTcc                                            <  2:645586/136‑1 (MQ=255)                                                                                               ggtggtCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTcc                                            >  1:645586/1‑136 (MQ=255)                                                                                                 tggtCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGc                                      <  1:505493/140‑1 (MQ=255)                                                                                                    tCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGa                                   >  2:849792/1‑140 (MQ=255)                                                                                                     cgcgTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGaa                                  <  1:849792/140‑1 (MQ=255)                                                                                                       cgTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAg                                >  1:111994/1‑140 (MQ=255)                                                                                                        gTTTCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAgg                               <  1:811738/140‑1 (MQ=255)                                                                                                          ttCGCTGATGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGgtg                             <  1:588444/140‑1 (MQ=255)                                                                                                                 aTGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGa                      <  2:503371/140‑1 (MQ=255)                                                                                                                 aTGGAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGa                      <  1:437739/140‑1 (MQ=255)                                                                                                                   ggAAATCACCGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGAtc                    <  2:142453/140‑1 (MQ=255)                                                                                                                           ccGAAGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGaa            >  2:353764/1‑140 (MQ=255)                                                                                                                              aaGACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAacac         <  2:636901/140‑1 (MQ=255)                                                                                                                                gACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCa       >  1:296803/1‑140 (MQ=255)                                                                                                                                gACGGTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCa       <  1:670813/140‑1 (MQ=255)                                                                                                                                   ggTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAgcg    >  1:595139/1‑140 (MQ=255)                                                                                                                                    gTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAgcgc   >  1:626971/1‑140 (MQ=255)                                                                                                                                    gTTACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAgcgc   >  1:100286/1‑140 (MQ=255)                                                                                                                                     ttACACCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGcgcg  >  1:30856/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCG  >  NC_000913/3545908‑3546179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑