Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,557,322	T→C	N253S (AAC→AGC)	rtcB ←	RNA‑splicing ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,557,322	0	T	C	100.0%	248.4 / NA	71	N253S (AAC→AGC)	rtcB	RNA‑splicing ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/35);  total (36/35)

TACGCGTCACGTAGATCTCTTCACCAAAGTGCTGTTCTTTTTGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGC  >  NC_000913/3557185‑3557450                                                                                                                                          |                                                                                                                                 tACGCGTCACGTAGATCTCTTCACCAAAGTGCTGTTCTTTTTGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGctt                                                                                                                                >  1:456697/1‑140 (MQ=255)               aTCTCTTCACCAAAGTGCTGTTCTTTTTGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATcatcgcatc                                                                                                                  <  2:636589/140‑1 (MQ=255)                    ttCACCAAAGTGCTGTTCTTTTTGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGt                                                                                                             >  1:526951/1‑140 (MQ=255)                    ttCACCAAAGTGCTGTTCTTTTTGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGt                                                                                                             <  2:675020/140‑1 (MQ=255)                    ttCACCAAAGTGCTGTTCTTTTTGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGt                                                                                                             >  2:630095/1‑140 (MQ=255)                     tCACCAAAGTGCTGTTCTTTTTGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATcatcgcatc                                                                                                                  >  1:715686/1‑134 (MQ=255)                     tCACCAAAGTGCTGTTCTTTTTGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATcatcgcatc                                                                                                                  <  2:715686/134‑1 (MQ=255)                     tCACCAAAGTGCTGTTCTTTTTGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGTGGtt                                                                                                            >  2:797982/1‑140 (MQ=255)                     tCACCAAAGTGCTGTTCTTTTTGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGtt                                                                                                            >  2:365045/1‑140 (MQ=255)                     tCACCAAAGTGCTGTTCTTTTTGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGtt                                                                                                            >  2:142985/1‑140 (MQ=255)                            aGTGCTGTTCTTTTTGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTg                                                                                                     >  2:43232/1‑140 (MQ=255)                            aGTGCTGTTCTTTTTGCACATAGTTGTGGTGACAGTTGATCTCTTCCAGCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCTATGCCGTTACTACGCTTTCCATCATCGCATCGCGGTTAAGGCTg                                                                                                     >  1:395518/1‑140 (MQ=255)                              tGCTGTTCTTTTTGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGc                                                                                                   >  2:578912/1‑140 (MQ=255)                                cTGTTCTTTTTGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGTTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCaa                                                                                                 >  1:642395/1‑140 (MQ=255)                                cTGTTCTTTTTGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCaa                                                                                                 >  2:688911/1‑140 (MQ=255)                                 tGTTCTTTTTGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCaaa                                                                                                <  1:578912/140‑1 (MQ=255)                                      tttttGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCt                                                                                           <  1:365045/140‑1 (MQ=255)                                      tttttGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCt                                                                                           <  1:688911/140‑1 (MQ=255)                                      tttttGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTCTTCTGCGTAATGCTCTGCAATGCTGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCt                                                                                           <  2:535469/140‑1 (MQ=255)                                       ttttGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTg                                                                                          >  1:18145/1‑140 (MQ=255)                                          tGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTgcgc                                                                                       >  1:707865/1‑140 (MQ=255)                                           gCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGcc                                                                                      <  1:142985/140‑1 (MQ=255)                                           gCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGcc                                                                                      <  1:797982/140‑1 (MQ=255)                                             acaTAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGcc                                                                                      >  2:26503/1‑138 (MQ=255)                                             acaTAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGcc                                                                                      <  1:26503/138‑1 (MQ=255)                                             acaTAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCa                                                                                    >  1:466708/1‑140 (MQ=255)                                             acaTAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCa                                                                                    >  1:420521/1‑140 (MQ=255)                                                tAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGc                                                                                 >  1:161543/1‑140 (MQ=255)                                                tAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGc                                                                                 >  1:751078/1‑140 (MQ=255)                                                  gTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCa                                                                               <  2:479505/140‑1 (MQ=255)                                                    tgtgGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACg                                                                             <  1:630095/140‑1 (MQ=255)                                                     gtggtgACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACgg                                                                            <  1:157527/140‑1 (MQ=255)                                                      tggtgACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGcc                                                                                      <  1:59937/129‑1 (MQ=255)                                                      tggtgACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGcc                                                                                      >  2:59937/1‑129 (MQ=255)                                                       ggtgACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCt                                                                          <  2:526951/140‑1 (MQ=255)                                                         tgACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACg                                                                             <  1:735194/135‑1 (MQ=255)                                                         tgACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACg                                                                             >  2:735194/1‑135 (MQ=255)                                                         tgACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCttt                                                                        <  2:707865/140‑1 (MQ=255)                                                           aCAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCa                                                                      >  2:72825/1‑140 (MQ=255)                                                             aGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAgg                                                                    >  1:167986/1‑140 (MQ=255)                                                              gTTGATCTCTTCCATCGCCAGCGTTTGTGGTTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGt                                                                   <  2:642395/140‑1 (MQ=255)                                                                   tctcTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAatca                                                              <  2:56868/140‑1 (MQ=255)                                                                       ttCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCttt                                                                        >  2:21360/1‑126 (MQ=255)                                                                       ttCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCttt                                                                        <  1:21360/126‑1 (MQ=255)                                                                       ttCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCaa                                                          >  2:368786/1‑140 (MQ=255)                                                                        tCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCaaa                                                         <  2:751078/140‑1 (MQ=255)                                                                        tCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCaaa                                                         <  2:456697/140‑1 (MQ=255)                                                                            tCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTAt                                                     >  2:33470/1‑140 (MQ=255)                                                                               ccAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTcc                                                  <  1:72825/140‑1 (MQ=255)                                                                                 aGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGt                                                >  2:88764/1‑140 (MQ=255)                                                                                   cGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTAc                                              <  2:428934/140‑1 (MQ=255)                                                                                   cGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTAc                                              <  1:88764/140‑1 (MQ=255)                                                                                   cGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTAc                                              <  1:748409/140‑1 (MQ=255)                                                                                    gTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTAcc                                             <  1:791798/140‑1 (MQ=255)                                                                                       tgtgGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTc                                          >  2:179126/1‑140 (MQ=255)                                                                                              gTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAg                                   >  2:796023/1‑140 (MQ=255)                                                                                                cTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTa                                 <  1:179126/140‑1 (MQ=255)                                                                                                         ttCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATaaa                                    >  2:560565/1‑128 (MQ=255)                                                                                                         ttCTGCGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATaaa                                    <  1:560565/128‑1 (MQ=255)                                                                                                              cGTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCAcgcg                   >  1:547997/1‑140 (MQ=255)                                                                                                               gTAATGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGa                  >  1:406345/1‑140 (MQ=255)                                                                                                                   tGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGc              <  1:52845/140‑1 (MQ=255)                                                                                                                   tGCTCTGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGc              <  1:796023/140‑1 (MQ=255)                                                                                                                        tGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGt         >  1:496092/1‑140 (MQ=255)                                                                                                                        tGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGt         >  1:831395/1‑140 (MQ=255)                                                                                                                        tGCAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGt         <  1:368786/140‑1 (MQ=255)                                                                                                                          cAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGtct       <  2:496092/140‑1 (MQ=255)                                                                                                                          cAATGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGtct       <  2:406345/140‑1 (MQ=255)                                                                                                                             tGCCGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCaa    >  2:168014/1‑140 (MQ=255)                                                                                                                               ccGTTACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGc  >  1:854536/1‑140 (MQ=255)                                                                                                                                  ttACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGc  >  1:752084/1‑137 (MQ=255)                                                                                                                                  ttACCACGCTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGc  <  2:752084/137‑1 (MQ=255)                                                                                                                                          |                                                                                                                                 TACGCGTCACGTAGATCTCTTCACCAAAGTGCTGTTCTTTTTGCACATAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGC  >  NC_000913/3557185‑3557450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑