Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	376,203	C→T	P249S (CCA→TCA)	mhpT →	3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	376,203	0	C	T	100.0%	183.6 / NA	53	P249S (CCA→TCA)	mhpT	3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/19);  total (34/19)

CACCACTGCGTGCCTTATTTGCGCCAGAAACGGCAACCGCGACGCTGCTGCTGTGGTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGTTAGCTTCG  >  NC_000913/376066‑376337                                                                                                                                          |                                                                                                                                       caccacTGCGTGCCTTATTTGCGCCAGAAACGGCAACCGCGACGCTGCTGCTGTGGTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCa                                                                                                                                      >  1:70068/1‑140 (MQ=255)  accacTGCGTGCCTTATTTGCGCCAGAAACGGCAACCGCGACGCTGCTGCTGTGGTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCAt                                                                                                                                     >  1:130745/1‑140 (MQ=255)    cacTGCGTGCCTTATTTGCGCCAGAAACGGCAACCGCGACGCTGCTGCTGTGGTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCg                                                                                                                                   >  1:732083/1‑140 (MQ=255)           tGCCTTATTTGCGCCAGAAACGGCAACCGCGACGCTGCTGCTGTGGTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAggcgg                                                                                                                            <  2:221646/140‑1 (MQ=255)           tGCCTTATTTGCGCCAGAAACGGCAACCGCGACGCTGCTGCTGTGGTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAggcgg                                                                                                                            >  2:339031/1‑140 (MQ=255)            gCCTTATTTGCGCCAGAAACGGCAACCGCGACGCTGCTGCTGTGGTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAggcggc                                                                                                                           <  1:298142/140‑1 (MQ=255)             ccTTATTTGCGCCAGAAACGGCAACCGCGACGCTGCTGCTGTGGTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCa                                                                                                                          >  1:762575/1‑140 (MQ=255)                                   aaCCGCGACGCTGCTGCTGTGGTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCaaa                                                                                                    >  1:638814/1‑140 (MQ=255)                                        cgACGCTGCTGCTGTGGTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATgggg                                                                                               >  1:259715/1‑140 (MQ=255)                                                ctgctgTGGTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTCGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAg                                                                                       <  2:762575/140‑1 (MQ=255)                                                   ctgTGGTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCgg                                                                                    <  2:863184/140‑1 (MQ=255)                                                        gTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCgg                                                                                    <  2:50402/135‑1 (MQ=255)                                                        gTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCgg                                                                                    >  1:50402/1‑135 (MQ=255)                                                        gTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGt                                                                               >  1:207433/1‑140 (MQ=255)                                                        gTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGt                                                                               <  2:638814/140‑1 (MQ=255)                                                                aTTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTg                                                                       >  2:860914/1‑140 (MQ=255)                                                                      tcACTCTGCTGGTGGTCTACATGTTGATCATCTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGGCGTTAATGTTgg                                                                      >  2:772065/1‑135 (MQ=255)                                                                      tcACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTgg                                                                      <  1:772065/135‑1 (MQ=255)                                                                      tcACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCa                                                                 >  1:479198/1‑140 (MQ=255)                                                                      tcACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCa                                                                 <  2:479198/140‑1 (MQ=255)                                                                          tctGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGa                                                             >  1:853335/1‑140 (MQ=255)                                                                           ctgctgGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGAt                                                            >  1:110467/1‑140 (MQ=255)                                                                             gctgGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATgg                                                          >  1:424902/1‑140 (MQ=255)                                                                              ctgGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGa                                                         >  1:69837/1‑140 (MQ=255)                                                                                 gtggtCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATaa                                                      <  2:69837/140‑1 (MQ=255)                                                                                    gtCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCAtt                                                               <  2:483901/128‑1 (MQ=255)                                                                                    gtCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCAtt                                                               >  1:483901/1‑128 (MQ=255)                                                                                     tCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTg                                                  <  2:110467/140‑1 (MQ=255)                                                                                      cTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGc                                                 >  1:789273/1‑140 (MQ=255)                                                                                            gTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAg                                           <  2:324987/140‑1 (MQ=255)                                                                                               gATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTg                                                  <  2:246512/130‑1 (MQ=255)                                                                                               gATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTg                                                  >  1:246512/1‑130 (MQ=255)                                                                                               gATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTaa                                        >  1:760739/1‑140 (MQ=255)                                                                                               gATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTaa                                        >  1:666667/1‑140 (MQ=255)                                                                                               gATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTaa                                        >  1:127948/1‑140 (MQ=255)                                                                                               gATCAACTGGCTACCGCTACTTTTGGGGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGAGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTaa                                        >  2:538683/1‑140 (MQ=255)                                                                                                   aaCTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCAt                                    >  1:462945/1‑140 (MQ=255)                                                                                                   aaCTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCAt                                    >  2:208244/1‑140 (MQ=255)                                                                                                   aaCTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCAt                                    >  2:769266/1‑140 (MQ=255)                                                                                                    aCTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATg                                   >  2:364417/1‑140 (MQ=255)                                                                                                     cTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGt                                  <  2:717840/140‑1 (MQ=255)                                                                                                       ggCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCg                                >  1:125372/1‑140 (MQ=255)                                                                                                          tACCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTa                             <  1:201589/140‑1 (MQ=255)                                                                                                           aCCGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTAc                            >  2:290011/1‑140 (MQ=255)                                                                                                             cGCTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTg                          <  2:666667/140‑1 (MQ=255)                                                                                                               cTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTa                             <  1:794874/135‑1 (MQ=255)                                                                                                               cTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTa                             >  2:794874/1‑135 (MQ=255)                                                                                                               cTACTTTTGGTGGAGCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGAt                        >  1:592852/1‑140 (MQ=255)                                                                                                                             gCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGt          >  2:586297/1‑140 (MQ=255)                                                                                                                             gCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGt          <  2:125372/140‑1 (MQ=255)                                                                                                                             gCAAGGATTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGt          >  1:529804/1‑140 (MQ=255)                                                                                                                                   aTTCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGTTAGCtt    <  2:529804/140‑1 (MQ=255)                                                                                                                                     tCCAGTCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGTTAGCTTCg  >  1:22143/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       CACCACTGCGTGCCTTATTTGCGCCAGAAACGGCAACCGCGACGCTGCTGCTGTGGTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGTTAGCTTCG  >  NC_000913/376066‑376337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑